FastQCFastQC Report
Tue 5 Sept 2023
SRR15324714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11290657
Total Bases322.9 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC147604913.073189629266038Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC7333606.495281895464541Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC3322612.942795977240297No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2994702.6523700082289277No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2503292.2171340427753674No Hit
GAGTTCTACAGTCCGACGATC2289912.0281459263176624Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTCC2011271.78135780761031No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1894981.6783611440857695No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1866421.6530658933310967No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1763301.5617337414465784No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1378291.2207349846868965No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1171531.03761012313101No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG787520.6974970544229623No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA741850.6570476811048286No Hit
ACAGAGTTCTACAGTCCGACGATC701860.621629015919977Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC697470.6177408453733029No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC683990.605801770437274No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT656250.5812327838849414No Hit
TTCAGAGTTCTACAGTCCGACGATC548270.48559618806948085Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCGGCCGAGGTGGGATCCCGAGGCCT528930.4684669811508755No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT521090.46152318682606336No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC517670.45849413368947445No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG509460.45122263478555763No Hit
CAGAGTTCTACAGTCCGACGAT499740.4426137469236733Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG497300.44045266807768585No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC495830.4391507066417836No Hit
TCAGAGTTCTACAGTCCGACGATC492510.43621022231035805Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC472680.4186470282464519No Hit
CCCCCGCGGGGGCGCGCCGGC451080.3995161663311533No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG441580.39110212984062837No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC408050.3614050094693338No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC375670.3327264303574185No Hit
CCAGAGTTCTACAGTCCGACGATC368160.3260749130896457Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG356800.3160134968230813No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC341840.30276360357063364No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC323270.28631637645178665No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG320270.28365931229688407No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG319400.2828887636919623No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC315480.2794168665295563No Hit
AAGAGTTCTACAGTCCGACGATC307310.2721807951477049Illumina DpnII expression Sequencing Primer (95% over 23bp)
CAGAGTTCTACAGTCCGACGATCA280220.24818750582893448Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC264670.23441505662602272No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC253420.224451066045138No Hit
GTTCTACAGTCCGACGATCA234850.20800383892629098No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT229880.20360196930966903No Hit
CAGAGTTCTACAGTCCGACGA222770.19730472726254988Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG221310.19601162270716396No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC218380.1934165567158758No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC214690.1901483678053456No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC209880.18588820827698513No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT204930.18150405242139583No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG204250.1809017845462846No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC203780.18048551116201653No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT201660.17860785249255204No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG200680.1777398782019505No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC187850.1663765004994838No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG184220.16316145287205164No Hit
AAGTTCTACAGTCCGACGATC181880.16108894283122763Illumina DpnII expression Sequencing Primer (95% over 21bp)
GATCCAGAGTTCTACAGTCCGACGATC166280.14727220922573417Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCG159490.14125838735513796No Hit
GCCGGCCGAGGTGGGATCCCGAGG157660.1396375782206474No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG147600.13072755642120737No Hit
AGAGTTCTACAGTCCGACGAT146510.1297621564449261Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGGCCGAGGTGGGATCCCGAGGCCT144770.1282210592350826No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG144230.12774278768720013No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT144170.1276896464041021No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA137240.1215518282062771No Hit
ATCCAGAGTTCTACAGTCCGACGATC135740.1202232961288258Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC135610.12010815668211336No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT134610.11922246863047917No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT134580.11919589798893013No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC127940.11331492932607909No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCT126540.1120749660537912No Hit
AGAGTTCTACAGTCCGACGATCA125870.1114815550591963Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG125040.11074643397633992No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC123800.10964818079231352Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC121680.10777052212284902Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC115840.1025981039013053Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC115670.10244753693252749Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGT115450.10225268556116796No Hit
CAGTTCTACAGTCCGACGATC114070.10103043604991278Illumina DpnII expression Sequencing Primer (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)