FastQCFastQC Report
Tue 5 Sept 2023
SRR15324715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37838340
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC32494528.587723457212974Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC17997754.756485088933606Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC9523872.5169893816694917No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8217102.171633322180624No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC7911032.090744467119858No Hit
AGTTCTACAGTCCGACGATC7708522.037224677403924No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC7078071.8706079600743584No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5650711.4933821092574358No Hit
GAGTTCTACAGTCCGACGATC5048221.3341547224323265Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3775330.9977525441126645No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC3418220.9033747252125754No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3385980.8948542668626583No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3350720.8855356762479538No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3210420.8484568826222292No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3172560.8384511582696281No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3056810.8078604928228881No Hit
ACAGAGTTCTACAGTCCGACGATC2984860.7888453880376358Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2641470.6980935210159853No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2456890.649312311269469No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC2360630.6238725060348842No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC2169330.5733153198581121No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2035140.5378512905164444No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1974420.5218040749144914No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1952230.5159396527437515No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1856690.4906901306981226No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1809500.47821865335530045No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1760840.46535868116835993No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1747530.4618410849947434No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1687460.44596565282726464No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1673360.4422392737102103No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC1561120.4125762388096307No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1446490.38228156943459995No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1429610.3778204857824101No Hit
AAGAGTTCTACAGTCCGACGATC1422380.37590972542664397Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1418890.3749873805246213No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1414710.3738826808998492No Hit
CAGAGTTCTACAGTCCGACGAT1413360.3735258999205568Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC1342630.35483321942770213No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1322960.349634788418308No Hit
CAGAGTTCTACAGTCCGACGATCA1159290.3063797196177211Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1114620.29457423343624484No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1091230.2883926726172448No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC1087010.2872774017041974No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT979800.25894370630424063No Hit
CCAGAGTTCTACAGTCCGACGATC936370.24746593005929965Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT910640.24066594887619278No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC899320.237674274294274No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC885760.23409060756893668No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG856250.226291639643811No Hit
CCCCCGCGGGGGCGCGCCGGC853540.22557543486315731No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC851160.22494644321077512No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG845090.2233422502149936No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA840890.2222322649460838No Hit
GTTCTACAGTCCGACGATCA832280.2199567951448187No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT831510.2197532978455186No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA815800.2156014243753822No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT788230.2083151639316101No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC767790.2029132356229158No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG745550.1970355993418316No Hit
AAGTTCTACAGTCCGACGATC745150.19692988645907827Illumina DpnII expression Sequencing Primer (95% over 21bp)
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG729080.19268287139446394No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC694090.18343563697561785No Hit
CAGAGTTCTACAGTCCGACGA687720.18175215931777133Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCT677600.1790776233841125No Hit
TCAGAGTTCTACAGTCCGACGATC661380.17479096598846566Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC657110.17366248096507406No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG646150.17076594797763325No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG640770.1693441097046012No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC639760.16907718467564908No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT635530.1679592709405328No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG632340.16711621070057514No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG606180.16020258816850846No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT591300.15627006893008522No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT582140.15384924391503432No Hit
AGAGTTCTACAGTCCGACGATCA552160.1459260633526735Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTC538390.1422868973638907No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC531930.1405796343074247No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT525490.13887765689509635No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC524050.13849709051718442No Hit
TTCAGAGTTCTACAGTCCGACGATC496750.1312821862692708Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGC488130.1290040736459369No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA487740.12890100358525242No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG480570.12700610016189928No Hit
AGAGTTCTACAGTCCGACGAT478030.12633482335641574Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT467980.12367878717723874No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC451130.11922563199125544No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG448680.1185781405843914No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC439470.11614410145899635No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG438610.11591681876107673No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG437650.11566310784246878No Hit
GATCCAGAGTTCTACAGTCCGACGATC437130.11552568109488948Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGT431570.11405627202461842No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC428820.11332949595568939No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT412360.10897941083039056No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC410360.10845084641662397No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG404050.10678322569119046No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT392820.1038153365078912No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC383750.10141829689145983No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC381740.10088708965562442No Hit
AGTTCTACAGTCCGACGATCA379410.10027131211358638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)