FastQCFastQC Report
Tue 5 Sept 2023
SRR15324716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23785580
Total Bases739.4 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC18346147.713135437521388Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC11628924.889063037352884No Hit
AGAGTTCTACAGTCCGACGATC9550914.015420267237545Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC9258983.8926862409913907No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC6192412.60343031366063No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5856582.462239726758818No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG4908582.063678918067165No Hit
AGTTCTACAGTCCGACGATC4541701.9094342034123195No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC4446201.8692838265873695No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG4073571.7126216808671473No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3749521.5763836744784026No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3292311.3841621688434758No Hit
GAGTTCTACAGTCCGACGATC2943351.2374514306567255Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2762401.1613759260863095No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2604971.0951887656302683No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2530761.0639891900891212No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2404831.0110453476434043No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC2330310.9797154410361235No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2297920.9660979467391587No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2255730.9483603090612043No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC1581160.6647557049271029No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1531010.6436715018090793No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1518140.6382606604505755No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1490340.6265729067779722No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1354990.5696686816129772No Hit
ACAGAGTTCTACAGTCCGACGATC1269730.5338234341983672Illumina DpnII expression Sequencing Primer (95% over 24bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1247740.5245783369587792No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT1162830.4888802375220617No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1162040.4885481035148186No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1078840.45356892705580437No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC1065990.4481664941531802No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC979440.41177890133433787No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT926390.3894754721137765No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC925620.389151746562413No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA891300.3747228362730697No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG848890.35689270558043995No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG836390.35163742065570824No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT802690.3374691724986315No Hit
CAGAGTTCTACAGTCCGACGAT791020.33256283849290197Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCCCGCGGGGGCGCGCCGGC783240.329291949155749No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG743430.31255491772746347No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC722780.30387318703180666No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC698070.29348453979259703No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG683790.2874809022945835No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG623110.26196964715596593No Hit
AAGAGTTCTACAGTCCGACGATC598470.2516104295123348Illumina DpnII expression Sequencing Primer (95% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC592520.2491089138881625No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC577390.24274791701526724No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG571590.24030946481019172No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC571210.2401497041484799No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG533870.2244511170213213No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT529430.2225844398160566No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT527030.22157542511050815No Hit
CAGAGTTCTACAGTCCGACGATCA525630.2209868331989382Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG524750.22061686114023707No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC479420.20155909588919002No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC475060.1997260525074436No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG458950.19295304129644936No Hit
TCAGAGTTCTACAGTCCGACGATC451890.18998485637096088Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTTCTACAGTCCGACGATCA441050.18542747328423356No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC426930.17949110343325664No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC419070.17618658027258532No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC416310.17502621336120455No Hit
CAGAGTTCTACAGTCCGACGA414490.17426104387616362Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTCC406460.17088504884051597No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC402930.16940095637777175No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT398710.1676267721871823No Hit
CCAGAGTTCTACAGTCCGACGATC396440.16667241244485104Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC385580.16210662090224412No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT384870.16180812071851935No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG378530.15914264020469546No Hit
TTCAGAGTTCTACAGTCCGACGATC375710.15795704792567597Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG369460.15532940546331012No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC364000.15303389700818731No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG363190.1526933545450647No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG355520.1494687117152493No Hit
AAGTTCTACAGTCCGACGATC347900.14626509002513288Illumina DpnII expression Sequencing Primer (95% over 21bp)
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG328950.1382980780792396No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTAT321650.13522899168319627No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT314160.13208002495629706No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA314080.13204639113277877No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC300920.12651362716402123No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC299250.12581152109807708No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC295040.12404154113542744No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC279220.11739045253468698No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT275620.11587693047636426No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC265400.11158020952190362No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG257630.10831352441269039No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT254110.10683363617788592No Hit
AGAGTTCTACAGTCCGACGAT249140.1047441348918126Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT246400.10359217643631143No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG241180.10139756945174346No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG238880.10043059702559282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)