FastQCFastQC Report
Tue 5 Sept 2023
SRR15324717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11804120
Total Bases318.5 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC196941816.684157734757015Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC127551210.805650908326923Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC7521926.372283575565143No Hit
GAGTTCTACAGTCCGACGATC4540163.846250292270834Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1535091.300469666523214No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1406491.1915246541038214No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1270191.0760564955286798No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1224371.0372395400927812No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC957280.8109710846721314No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC940490.796747237405245No Hit
ACAGAGTTCTACAGTCCGACGATC812740.6885223125485Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGAGTTCTACAGTCCACGATC809170.68549794478538No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG564010.47780774848103885No Hit
CAGAGTTCTACAGTCCGACGAT533400.4518761246073405Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG492040.4168375109707458No Hit
TCAGAGTTCTACAGTCCGACGATC459430.38921156342022956Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCAGAGTTCTACAGTCCGACGATC427860.36246666418165857Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGAGTTCTACAGTCCACGATC417660.3538256134298872No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC401210.3398898011880598No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG385570.32664019003534356No Hit
AAGAGTTCTACAGTCCGACGATC376110.3186260390439948Illumina DpnII expression Sequencing Primer (95% over 23bp)
TTCAGAGTTCTACAGTCCGACGATC366660.3106203596710301Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC358670.30385153658214253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG331790.2810798263657096No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG329530.27916524061090536No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT310300.262874318458301No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA305470.2587825267787857No Hit
AGAGTTCTACAGTCCGACGAT302140.2559614778568839Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCT298420.25281003581800254No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT281420.23840828456505014No Hit
CAGAGTTCTACAGTCCGACGATCC281290.238298153526057Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGTTCTACAGTCCGACGATC278880.2362564934954914Illumina DpnII expression Sequencing Primer (95% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG256200.21704286300037612No Hit
CCCCCGCGGGGGCGCGCCGGC240600.20382713832119634No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG234970.19905761717095385No Hit
CAGTTCTACAGTCCGACGATC231830.19639752899834975Illumina DpnII expression Sequencing Primer (95% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG226970.19228032246368218No Hit
AGAGTTCTACAGTCCGACGATCC218980.18551149937479455Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGTTATCGCAAGGCATTTACGTATGAGTGTCAGC211000.17875114790429106No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC201920.17105891841153767No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC201680.1708555995703195No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG195420.1655523664618794No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC180520.1529296550695859No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC176490.14951559286079777No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT172860.14644039538737322No Hit
CAGAGTTCTACAGTCCCACGATC172670.14627943463807552Illumina DpnII expression Sequencing Primer (95% over 23bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG172460.14610153065200965No Hit
GTTCTACAGTCCGACGATCC171910.14563559164088472No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC165980.14061192193911956No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC158390.13418196358559553Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC156130.1322673778307913No Hit
AACGATGCCGACCGGCGATGCGGCGGCG154210.13064082710104608No Hit
ACAGTTCTACAGTCCGACGATC149480.12663375160537169Illumina DpnII expression Sequencing Primer (95% over 22bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC148600.1258882491875718Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG147430.12489706983663332No Hit
ATCCAGAGTTCTACAGTCCGACGATC141900.12021226487023175Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC141040.11948370568920005Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC140560.11907706800676375Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCCAGAGTTCTACAGTCCGACGATC137330.1163407352687028Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGC136310.11547663019352565No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG133240.11287584334961014No Hit
AGTTCTACAGTCCGACGATCC130770.11078335360874No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT130110.11022422679539008No Hit
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC127490.10800466277875861No Hit
TACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC121700.10309959573437072Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC120210.10183732459514135No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC119110.1009054465728915Illumina DpnII expression Sequencing Primer (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)