FastQCFastQC Report
Tue 5 Sept 2023
SRR15324718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8723678
Total Bases253.4 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC102485311.747946221765636Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC6860857.864630033341441Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC3509084.022477675127395No Hit
GAGTTCTACAGTCCGACGATC2199082.5208174808836366Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1624781.8624942369491402No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1353261.5512493698185559No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1184421.3577071505848795No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1039541.1916304109344706No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1030251.1809812329157496No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC852310.977007633706792No Hit
CAGAGTTCTACAGTCCGACGAT661990.7588427725094852Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC642940.7370056528909023No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG637460.7307238987958978No Hit
ACAGAGTTCTACAGTCCGACGATC636080.7291419972172287Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC545410.6252064782767085No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA542550.6219280445701916No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC519130.5950815699524903No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG473170.542397369549862No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG463160.5309228515770527No Hit
AGAGTTCTACAGTCCGACGAT406170.46559490159999034Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG403530.462568655101667No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC399300.457719782871399No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG386890.44349413171829594No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC340820.3906838377115708No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG314630.36066209688161344No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC292660.33547776522700634No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC287290.3293221047360987No Hit
AAGAGTTCTACAGTCCGACGATC285730.32753386816890767Illumina DpnII expression Sequencing Primer (95% over 23bp)
CAGAGTTCTACAGTCCGACGATCC281300.32245573484028184Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG269680.3091356650256922No Hit
CCAGAGTTCTACAGTCCGACGATC267220.3063157535158909Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTCC258870.29674410265945167No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC250130.2867253926612147No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC249400.28588858965220865No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT229440.26300833203609764No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG220710.2530010850927785No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA220010.25219867124852613No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG216810.24853049367480093No Hit
AGAGTTCTACAGTCCGACGATCC213550.2447935377715684Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC205540.23561163078233746No Hit
CAGAGTTCTACAGTCCACGATC200910.2303042363553538No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGG200260.22955913778569087No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG198240.2272436006922768No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC197750.22668191100130014No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC194060.22245204373659827No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT192180.2202969894120347No Hit
AAGTTCTACAGTCCGACGATC191890.21996456081941584Illumina DpnII expression Sequencing Primer (95% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG188380.21594102854323602No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT186490.21377451116375454No Hit
TCAGAGTTCTACAGTCCGACGATC184950.2120092007063993Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG178600.20473016083353834No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC175720.20142880101718563No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA169180.19393196310088476No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT168300.19292321426811032No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC167370.19185715016074642No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG165570.18979380027552598No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC152190.17445623279538744No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG149000.17079951827658013No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC148770.17053586801346862No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC144170.16526286275123864No Hit
GTTCTACAGTCCGACGATCC141930.162695138449631No Hit
CAGAGTTCTACAGTCCGACGATCA140110.16060886245457479Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC140000.16048276885047796No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC139080.15942816779803198No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC138520.15878623672263006No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG136960.15699800015543902No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT132320.15167914267353746No Hit
GAGTTCTACAGTCCGACGAT126940.14551201912771197No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG125270.1435976889564241No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA123360.14140824546710687No Hit
CAGTTCTACAGTCCGACGATC122540.1404682749638398Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGA117590.1347940627794836Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC116870.13396872282539543No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC108280.12412195865092682No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA105940.12143960380014025No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC105780.12125619492145401No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG105770.12124473186653612No Hit
AGTTCTACAGTCCGACGATCC104170.1194106430796735No Hit
AGAGTTCTACAGTCCACGATC102650.11766825873215402No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGG102630.11764533262231824No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC102400.11738168235920675Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC102390.11737021930428886Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA101350.11617806159282815No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT101000.11577685467070196No Hit
TTCAGAGTTCTACAGTCCGACGATC100610.11532979552890421Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCTTGCGACCCGGGTTCGGTTCCCGGGCGG99290.11381667227974256No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG98710.11315181509450485No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT98480.11288816483139336No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC98150.11250988401910296No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG97940.11226915986582724No Hit
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC96070.11012556859618156Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC95130.10904804143389978Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCCAGAGTTCTACAGTCCGACGATC94910.10879585422570617Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC93270.10691591321917202No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC92340.10584984911180813No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC91620.10502450915771994Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC91310.10466915445526531No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG88950.10196387349464296No Hit
CCCCCGCGGGGGCGCGCCGGC87590.10040489802580976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)