FastQCFastQC Report
Tue 5 Sept 2023
SRR15324719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15873934
Total Bases491.1 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC11995437.556683806295276Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC7394564.658303354417374Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6033453.8008536510231172No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5128743.2309193171648563No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4946443.116076959876487No Hit
AGTTCTACAGTCCGACGATC4231052.6654073275093624No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3227382.033131799590448No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2932011.8470594623865768No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2830481.783099261972489No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2696001.6983817622021107No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2654491.672231974758116No Hit
GAGTTCTACAGTCCGACGATC2499501.574593922338344Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2099961.322898280917635No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1917071.2076842451278933No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1663441.0479065869871955No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1602391.0094473115486053No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1564020.9852756096881844No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1509260.9507788050523581No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1278170.8052005255911987No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1272470.801609733289807No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1175490.7405158670812163No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1139990.718152160642724No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1110340.6994737410398708No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC994940.62677594602573No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG896000.5644473512363097No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG860100.5418316593731585No Hit
ACAGAGTTCTACAGTCCGACGATC832930.5247155494031914Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC751650.47351211111246905No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC723380.45570304122468946No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT694090.4372514085040293No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT691590.43567649959991017No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC691140.4353930159971687No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC667980.42080305990940875No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC615800.38793156126263345No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG582670.36706086846524627No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC582420.3669033775748343No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT573650.3613785971391843No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA573190.36108881390082637No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG553570.3487289288212991No Hit
CAGAGTTCTACAGTCCGACGAT530720.33433426143764994Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG483970.3048834649306215No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC483900.30483936748130613No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC468690.2952576217086451No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC458960.2891280762538133No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC458390.28876899702367415No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG428970.2702354690399998No Hit
AAGAGTTCTACAGTCCGACGATC425380.26797389985368464Illumina DpnII expression Sequencing Primer (95% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT419910.26452799917147196No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG407740.25686134262621985No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC401860.2531571568837315No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT357230.22504188312739612No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC355480.2239394468945127No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA354250.22316459171368608No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC343160.2161782958150135No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG333700.21021884052182652No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT333610.21016214380127823No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT325210.20487044988343783No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC321910.20279157013000054No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC314340.1980227459683277No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG311580.19628404653818013No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC297340.18731336542031735No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT290840.1832186022696075No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT288840.1819586751463122No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC286120.18024517425863051No Hit
AAGTTCTACAGTCCGACGATC282970.1782607890394404Illumina DpnII expression Sequencing Primer (95% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCT279500.17607481548052298No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG279200.17588582641202866No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC276260.17403373354078455No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC272600.17172806690515408No Hit
AGAGTTCTACAGTCCGACGAT269080.16951059516815428Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG266320.16777189573800672No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT258640.16293377558455263No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT250530.15782477109959006No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA248470.15652704616259588No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG243970.15369221013518136No Hit
CCAGAGTTCTACAGTCCGACGATC241100.15188421471325256Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC230980.14550898346937816No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC230150.1449861137132106No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG224970.14172290246387567No Hit
TCAGAGTTCTACAGTCCGACGATC216400.13632411474055517Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC206850.13030796272681996No Hit
CAGAGTTCTACAGTCCGACGATCA206800.13027646454873756Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC205480.12944491264736266No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC187070.1178472834774291No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC183820.11579990190207419No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG181760.11450217696507999No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG170740.1075599785157227No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC169760.10694261422530799No Hit
CAGAGTTCTACAGTCCGACGATCC169750.10693631458969151Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA167250.10536140568557233No Hit
TCTACAGTCCGACGATCCAGAGT163880.1032384284828197No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC163160.10278485471843336No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT160910.10136743670472613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)