FastQCFastQC Report
Tue 5 Sept 2023
SRR15324720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13682529
Total Bases401 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC141019910.306566863479699Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC8465216.186875247989608Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC5216193.8122996121550337No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4521363.3044768258850397No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3936942.8773481861430734No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3706452.7088924861770804No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC3162092.311042059549079No Hit
GAGTTCTACAGTCCGACGATC3037782.220188972374917Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC2974662.174057149814921No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2471931.8066323849925698No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1842541.3466370142537245No Hit
GTGTTATCGCAAGGCATTTACGTAT1673841.2233410943254717No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1302410.9518781213619207No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC991390.7245663429618896No Hit
CCCCCGCGGGGGCGCGCCGGC971780.7102341972014091No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG965700.7057905742425249No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC918790.6715059767094227No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG855670.6253741541494267No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG714280.5220379945841884No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC674310.49282555878375994No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG617610.45138585125600683No Hit
CAGAGTTCTACAGTCCGACGAT574070.4195642486853125Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC568440.4154495122941088No Hit
ACAGAGTTCTACAGTCCGACGATC536960.39244206973725393Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC514810.3762535420169765No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT502360.3671543469778138No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC477480.3489705740802742No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG440670.32206765284400274No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG439660.32132948521431964No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG435990.3186472325401247No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC435000.3179236820912274No Hit
CTACAGTCCGACGATCCAGAGTT433680.3169589481593644No Hit
TCTACAGTCCGACGATCCAGAGT432740.31627194066243164No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC395450.2890182070873009No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA390740.28557586101224414No Hit
TCAGAGTTCTACAGTCCGACGATC372750.27242770689541385Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT350330.25604184723452805No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG345060.25219022009746883No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC329050.24048916687843305No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT324680.23729531287673497No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC323080.2361259384138707No Hit
TTCAGAGTTCTACAGTCCGACGATC314640.2299574881222616Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC314580.22991363657990418Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG308280.22530922463237607No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT308030.22512650987255353No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT289080.21127673107800465No Hit
CAGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATC278350.20343461358642104Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC274010.2002626853559017No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT269260.19679110491927335No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC267940.19582637098741032No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT248550.18165501421557373No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT246040.1798205580269554No Hit
CAGAGTTCTACAGTCCGACGATCCTACAGTCCGACGATC244710.17884851550469946Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGTTCTACAGTCCGACGATC241500.17650245798857797Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGAGTTCTACAGTCCGACGAT224720.1642386433092888Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT224710.1642313347188959No Hit
CAGAGTTCTACAGTCCGACGATCTACAGTCCGACGATC218240.15950267673468843Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG214490.15676195533735027No Hit
CAGAGTTCTACAGTCCGACGATCC214220.15656462339674193Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCACAGTCCGACGATC213640.15614072515395364Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC211030.15423318306140626Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC209290.15296148833304135Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG206490.15091508302302886No Hit
CCAGAGTTCTACAGTCCGACGATC204440.149416821992484Illumina DpnII expression Sequencing Primer (95% over 24bp)
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC202460.14796972109468945Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCAGTTCTACAGTCCGACGATC201630.1473631080920786Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC196750.14379651598034254No Hit
CAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC194990.1425102040711918Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC193510.14142853269304234Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC192540.14071959942493087No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG191790.14017145514546325No Hit
AAGTTCTACAGTCCGACGATC189850.13875358860924028Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGATCTTCTACAGTCCGACGATC188090.1374672767000896Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCCAGAGTT183130.1338422158652103Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCCAGTCCGACGATC174170.12729371887317031Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC172230.12587585233694737No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCT171230.1251449932976572No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC166730.12185612762085136No Hit
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC165420.12089870227938125No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT165390.12087677650820254No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC163750.11967816768376664No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC159510.1165793253571763No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC158080.11553419693099135No Hit
TACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC157190.11488373238602308Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCCCCGCGGGGGCGCGCCGG155000.1132831510899776No Hit
AGAGTTCTACAGTCCGACGATCC151780.11092978498346322Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC149060.10894184839659393No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC145790.10655193933811506No Hit
CAGAGTTCTACAGTCCGACGATCCAGAGT143050.10454938557045997Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTCTACAGTCCGACGATC141060.10309497608227251Illumina DpnII expression Sequencing Primer (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)