FastQCFastQC Report
Tue 5 Sept 2023
SRR15324721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19110405
Total Bases571.3 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC14721417.703347992886597Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC8397984.394454225329081Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5565252.9121570160339356No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4532812.3719068224875404No Hit
AGTTCTACAGTCCGACGATC4225002.2108374992576034No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3349401.7526577798848322No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2753041.4405974127706869No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2635671.3791806086788845No Hit
GAGTTCTACAGTCCGACGATC2614351.3680243825287848Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2256211.1806186211124254No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1941041.0156979927950245No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1680840.8795417993496214No Hit
GTGTTATCGCAAGGCATTTACGTAT1531940.801626129849158No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1493540.7815323641754323No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC1318640.6900115408333838No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1223320.6401329537495412No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1157240.6055549319860045No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1102480.5769003848950349No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1056770.5529814778912325No Hit
CAGAGTTCTACAGTCCGACGAT1046300.5475027870942557Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAGAGTTCTACAGTCCGACGATC1040190.5443055759414832Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1027860.5378535933696853No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1024140.5359070098200431No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC981450.5135683937624556No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG976100.5107688717219756No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC976030.5107322424616328No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG969010.5070588509244048No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT891810.4666620095178517No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC851830.4457414691106756No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG838890.43897028869874816No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG816910.4274687009511311No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG764710.40015373823840994No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC743690.389154494632636No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG720830.37719242475499604No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG695330.36384890848728746No Hit
CTACAGTCCGACGATCCAGAGTT677080.3542991370407901No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC676590.354042732218391No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC641720.33579612781623414No Hit
CCCCCGCGGGGGCGCGCCGGC630310.32982555838036925No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC625260.3271830188842152No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG590440.308962578239446No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT583920.3055508242760946No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC576820.30183557072704637No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA570530.2985441700476782No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC522600.2734635922158635No Hit
TCTACAGTCCGACGATCCAGAGT511700.2677598931053528No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC499780.2615224533441337No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC487260.25497104849426266No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC473910.2479853252717564No Hit
AAGAGTTCTACAGTCCGACGATC465530.24360027953358393Illumina DpnII expression Sequencing Primer (95% over 23bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCT437800.2290898596863855No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC409070.21405616469143382No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC396330.20738963930905704No Hit
AGAGTTCTACAGTCCGACGAT390540.20435987620356552Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTC384350.20112080303897276No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT377600.19758869579163812No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC375620.19655261099908664No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT373110.1952391903782259No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG363550.19023667996570454No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC357790.18722261511464566No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT351910.18414575724585638No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC341410.17865136819444694No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT336670.17617104399409642No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG294900.15431384107244195No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT294890.1543086083209644No Hit
CCAGAGTTCTACAGTCCGACGATC292970.15330392003727814Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATC291200.1523777230257548Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA287330.1503526482039496No Hit
AAGTTCTACAGTCCGACGATC275760.1442983547444442Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC267310.139876679745929Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCCAGAGTT266480.13944236137329377Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTT264280.13829115604823655No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG264120.138207432024596No Hit
CAGAGTTCTACAGTCCGACGATCA260480.1363027104867741Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCC257090.13452880773589046Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCAGAGTTCTACAGTCCGACGATC245610.12852160903968282Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGAGTTCTACAGTCCGACGATCTACAGTCCGACGATC244750.128071592412615Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCACAGTCCGACGATC241250.12624012939547855Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCCTACAGTCCGACGATC238780.12494763978052795Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC238570.12483775199949976No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG235150.12304815099418354No Hit
AACGATGCCGACCGGCGATGCGGCGGCG232960.12190217842060384No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT228970.11981431058106827No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG226540.11854275197202781No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA225340.11791482179472387No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC219940.11508913599685618No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG214980.1124936912639999No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT212960.11143667546553827No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT211400.11062036623504318No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG211250.11054187496288016No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC210060.10991917753705377No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT205210.10738129307045037No Hit
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC204940.10724000878055698Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC203570.10652312182813499Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC203220.10633997552642134No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC201800.10559692481661169Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCCAGAGT198140.10368173777583467Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG197320.103252652154677No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT195520.10231075688872109No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC193380.10119094807252908No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG193260.10112815505479869No Hit
CAGAGTTCTACAGTCCGACGA191790.10035894058760136Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGT191220.10006067375338198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)