FastQCFastQC Report
Tue 5 Sept 2023
SRR15324722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27031867
Total Bases850.4 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC17332396.41183607480756Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC11240914.158392019315573No Hit
AGAGTTCTACAGTCCGACGATC10861874.018172329717366Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC9017533.3358887123852745No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG7354592.7207110777809023No Hit
AGTTCTACAGTCCGACGATC6647332.4590717318933244No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC5598652.0711296041816127No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5065921.8740547961411618No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC4790291.7720899559027867No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3993831.4774525192802999No Hit
GAGTTCTACAGTCCGACGATC3926881.4526854545414862Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTA3893151.4402075890651578No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3330821.232182741946755No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3258131.2052922574678249No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3215591.1895552756307954No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3081261.1398620746395356No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG2772091.0254896563378326No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2416580.8939745079390928No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2223040.8223775294544029No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1903700.704242884888417No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1759490.6508947384211382No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1700460.629057549003182No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1436010.5312285681192497No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1278240.47286411996626054No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1251950.46313856160952555No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1232560.45596554614596174No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1185610.43859715645981834No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC1177690.4356672811389609No Hit
GTGTTATCGCAAGGCATTTACGTAT1173690.43418754612842686No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1149280.4251574632266429No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1146750.42422153083248004No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1121020.4147031353772198No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1120260.4144219857252184No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1033210.38221925255847106No Hit
CAGAGTTCTACAGTCCGACGAT1026940.37989976792945895Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT1024650.37905261963592823No Hit
ACAGAGTTCTACAGTCCGACGATC886300.32787228495908183Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT883620.326880862502024No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG869090.3215057250762591No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT852990.3155497916588595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC842400.31163219321847063No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG827700.306194167054758No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG766570.28358011675627137No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC724450.26799850709534784No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT713930.2641068040176433No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC683850.2529791967384273No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC672610.24882114135882663No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG638630.23625079244433989No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG633760.23444921506901467No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC611140.22608131358444458No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG592060.2190229775841972No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG572770.21188695549589676No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT555490.20549450025038968No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG550190.20353385136143207No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC547930.20269780108048033No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC498980.1845895438890699No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC492920.18234774534811082No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC491410.1817891453816342No Hit
CCCCCGCGGGGGCGCGCCGGC483750.1789554528364615No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG483530.17887406741088213No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT477360.17659157615713336No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC465730.17228924661400563No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC453130.16762808133082335No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG441690.16339603920069598No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC434680.16080280359473506No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGC434190.16062153605594465No Hit
AGAGTTCTACAGTCCGACGAT423460.15665214689018706Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC421420.15589748203481468Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGAGTTCTACAGTCCGACGATC415360.1536556834938556Illumina DpnII expression Sequencing Primer (95% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC410620.15190219750637277No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG409010.15130660416463282No Hit
CTACAGTCCGACGATCCAGAGTT406820.1504964492463654No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA403170.14914619104925309No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA399410.1477552401393511No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC393120.1454283568352863No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC382470.1414885623697394No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC379550.14040835581204952No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG377020.13947242341788674No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT367710.13602834018086876No Hit
CAGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATC358920.13277662249522018Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC352860.1305348239542611No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG346810.12829672475082834No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG338060.1250598044152851No Hit
TCTACAGTCCGACGATCCAGAGT320020.11838619951777656No Hit
TCAGAGTTCTACAGTCCGACGATC318240.11772771743808891Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC317870.11759084194961451No Hit
AAGTTCTACAGTCCGACGATC314630.11639225659108192Illumina DpnII expression Sequencing Primer (95% over 21bp)
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG314070.11618509368960717No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA310990.11504569773149595No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT297910.11020696424704961No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT295970.10948929276694058No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC295840.10944120137909824No Hit
CAGAGTTCTACAGTCCGACGATCC291550.10785418558030045Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG287900.10650392738318815No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC287770.10645583599534578No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA286370.10593792874165886No Hit
CAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC285650.10567157643976274No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC283550.10489471555923237No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC279220.10329290241032926No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC277120.10251604152979889Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCAGTTCTACAGTCCGACGATC273790.1012841621335293Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC273540.1011916786953709Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC271880.10057758866599928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)