FastQCFastQC Report
Tue 5 Sept 2023
SRR15324723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31418299
Total Bases980.1 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC18603205.921135323080349No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC18592835.917834698816763No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC11745563.7384455472907683No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC10224793.254405975320306No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC9369922.9823129508061528No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC9205362.9299358313446566No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG7882122.5087672633072846No Hit
CAGAGTTCTACAGTCCGACGATC6088591.9379120429148629Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC5847401.8611446787746213No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5106711.6253935326034041No Hit
AGAGTTCTACAGTCCGACGATC4256501.35478372014984Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4183951.3316920817387345No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG4077031.2976609586661583No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3758421.19625190402574No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3268051.0401740718044603No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG3104310.9880579467398919No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC3095370.98521247124168No Hit
AGTTCTACAGTCCGACGATC2815270.8960606046813674No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG2777920.8841726281871594No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2589510.8242043912052655No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2541190.8088248189375242No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC2397160.7629821079747188No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC2371050.7546716644335201No Hit
CCCCCGCGGGGGCGCGCCGGC2367390.7535067382228428No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC2329400.741415058784691No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2184310.695234964820979No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2156650.6864311782124168No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2007110.638834712216597No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1957880.6231654998254361No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1776630.5654761895289111No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1721160.5478208734343002No Hit
GAGTTCTACAGTCCGACGATC1633550.519935850123522Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCT1625040.517227237540772No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG1608880.5120837382061963No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1580130.5029330200212303No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1522450.4845742921983141No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1452040.46216378550601994No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT1323790.4213436252548236No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1299490.4136092791019653No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1268050.40360237198073645No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1180220.37564732578297766No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC1084570.345203284238908No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1065120.3390126244581223No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1050220.3342701652944356No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1039880.3309790896063469No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG934180.29733627527066314No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC918720.29241557603102575No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC817120.2600777336799806No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT794610.25291311919846454No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC792930.25237839897061265No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC788460.2509556612215066No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC771930.24569439612246355No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT760850.2421677889054401No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC757030.2409519369587768No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC745410.23725345538280093No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG706140.22475437005676216No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC688720.2192098305512975No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC654840.20842630595628361No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC652780.20777063710546517No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC626830.19951111930025237No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC595230.18945328644303755No Hit
AACGATGCCGACCGGCGATGCGGCGGCG549400.1748662459415769No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT545640.17366949114590832No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC544370.17326526811652024No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT540370.1719921247168728No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG528120.1680931230554525No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT527410.1678671401020151No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCT490460.15610647794777177No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT480860.15305093378861792No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC478500.15229977918282592No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA456830.14540252481523586No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCC453220.14425351289705404No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT439220.13979751099828797No Hit
CCCCCGCGGGGGCGCGCCGG424570.1351346232970792No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT422810.13457444020123432No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT421910.13428798293631364No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG416860.13268063939425873No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC401580.12781723160760547No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG401010.12763580867315572No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG399650.12720293991727558No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG384940.12252095506507212No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC374210.11910574789551784No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA360390.1147070374497359No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG359250.11434419158083639No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG356590.11349755122007081No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG354600.1128641623787462No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC347720.11067435573135262No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG346860.1104006299004284No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT343040.10918477795376509No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC338520.10774612591216348No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT331130.10539399348131481No Hit
CGCCGGCCGAGGTGGGATCCCGAGGC330300.10512981622588798No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG330170.10508843906539944No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG318580.10139950606492096No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)