FastQCFastQC Report
Tue 5 Sept 2023
SRR15324724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58636936
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC20823883.551324714511004No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC17325442.954697360039413No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC16896802.8815966782438975No Hit
CAGAGTTCTACAGTCCGACGATC15612882.6626357147992863Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC14658952.4999515663642455No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG12490862.130203392619287No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG11249821.918555226009763No Hit
AGAGTTCTACAGTCCGACGATC10212331.7416206740406763Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTA8849931.5092756551945348No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC7458411.271964483273819No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC7331731.2503603530716545No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC6934681.182647060549003No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG6764821.153678971220461No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC6730841.1478839890269847No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG6548041.1167090995341231No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG6537771.1149576437622866No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC5453310.930012782386856No Hit
AGTTCTACAGTCCGACGATC5386830.9186752186369355No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA5042980.8600347057697558No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC5042770.8599988921658527No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT4906680.8367899714268835No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG4738510.8081100963392768No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC4705270.8024413144643164No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG4303760.7339674092111498No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG4275740.7291888512046399No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG4252200.7251743167480648No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT4113590.7015356327622575No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3817990.6511237217442604No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG3758890.6410447503600802No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3737460.6373900573522464No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC3535030.6028674485992924No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC3526650.6014383152625846No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC3519880.6002837528891346No Hit
GAGTTCTACAGTCCGACGATC3236610.5519746120431668Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCG3001530.5118838405881235No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC2881060.49133876981566704No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC2832070.4829839676479685No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2519970.42975813060900725No Hit
CCCCCGCGGGGGCGCGCCGGC2511420.428300005307235No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC2409370.41089629922000015No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT2253250.3842714428325518No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2244990.38286277441236016No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT2222860.37908870272484907No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC2194310.37421975800372653No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2109730.35979540267929416No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT2103110.35866642145148925No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2080950.3548872335348491No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2061060.3514951736223052No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2025500.3454307366947004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2014010.3434712209382837No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT1982610.33811623444990374No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC1954990.33340589283178096No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA1940340.3309074676071069No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1822270.3107716951649725No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1767260.30139023635204953No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT1747650.2980459279113766No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC1694170.2889253967840339No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA1614920.2754100248348584No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1545800.26362223292158377No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC1530770.26105900212794203No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT1404710.23956060732777718No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT1395830.2380462035055856No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC1391260.23726683126826406No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1386550.23646358329500708No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT1370040.23364795186433343No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1344000.22920706498033935No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC1341960.22885916139956564No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCG1337210.22804909178746996No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1326440.22621236553014978No Hit
ACAGAGTTCTACAGTCCGACGATC1324000.22579624556098907Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG1268970.21641137592864676No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1266000.21590486924487323No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1253870.21383620726703728No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1246400.21256226621390995No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG1234290.21049701505549334No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1159200.19769109354554268No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT1148390.19584754564938386No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT1129000.19254075622232378No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1123690.19163518366648624No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT1051330.1792948390072769No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC1047710.17867748069237452No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG1040280.17741036127808588No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1006010.17156592220302916No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1004630.171330575663094No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT984670.1679265778825824No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT979160.1669868971325514No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG969020.1652576116869408No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA952550.16244880189510585No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC896270.15285075604905413No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC875610.14932737958886527No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG874780.14918583058296225No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG836490.14265581680461611No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC816120.13918189722600785No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT809530.13805803222733193No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC792690.135186122276239No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC784220.13374164025214413No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT772150.13168321073256625No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA762880.1301022959316974No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTAT741970.12653628422876667No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGG740900.12635380538983143No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG726480.12389460458847987No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG688750.11746009375387555No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT664070.11325114259039729No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG639170.10900467241330618No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC637280.10868234997817759No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC635550.10838731409840377No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG632550.10787569118550125No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGG619790.10569958839595575No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG611850.1043454930864737No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC606770.10347914495395871No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT606480.10342968807237814No Hit
AAGAGTTCTACAGTCCGACGATC602700.10278504320212092Illumina DpnII expression Sequencing Primer (95% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATT597130.10183512999383187No Hit
CAGAGTTCTACAGTCCGACGAT595430.1015452103431871Illumina DpnII expression Sequencing Primer (100% over 22bp)
TAAGTGGGAGGCCCCCGGCGCCCCCCCGG593890.10128257724789713No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT587850.10025250978325335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)