FastQCFastQC Report
Tue 5 Sept 2023
SRR15324725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24002060
Total Bases717.8 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC250704210.445111794570966Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC17595597.3308666006167815Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC10318394.298960172585186No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC7480333.1165366639363454No Hit
GAGTTCTACAGTCCGACGATC6198482.5824783372760503Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5173162.1552983368927503No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4833422.013752152940206No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3953341.64708362532216No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3371021.4044711162291903No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3214301.3391767206648097No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2702901.1261116754145268No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2300120.9583010791573723No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2040020.8499353805465031No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2031500.8463856852286846No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1886190.7858450483000209No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1874590.7810121297921928No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1503820.6265378888312086No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1402440.5842998475964146No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1313700.5473280210115298No Hit
ACAGAGTTCTACAGTCCGACGATC1301590.5422826207417196Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1110230.4625561305987903No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1083030.45122376995974506No Hit
CAGAGTTCTACAGTCCGACGAT1031510.4297589456904949Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1025360.4271966656195343No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT954860.3978241867573033No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG941900.39242465021752304No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG919340.3830254569816091No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG853160.3554528236326382No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT850740.35444457684048786No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC770220.32089745630166744No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG722330.3009450022206427No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG697850.2907458776455021No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC678100.28251741725501894No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA642330.2676145297528629No Hit
AAGAGTTCTACAGTCCGACGATC641910.26743954477240706Illumina DpnII expression Sequencing Primer (95% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT632560.26354404580273527No Hit
AGAGTTCTACAGTCCGACGAT623950.2599568537033905Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC579580.24147094041094808No Hit
CCAGAGTTCTACAGTCCGACGATC559740.23320498323893868Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC557720.2323633888091272No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG543170.22630140912904975No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT541730.2257014606246297No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT531640.221497654784631No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC530690.2211018554240761No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG495750.20654477157377324No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG468390.19514574998979253No Hit
AAGTTCTACAGTCCGACGATC467470.1947624495564131Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGATCC460470.19184603321548235Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC458020.19082528749615657No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC455410.18973788083189527No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC451010.18790470484616736No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC442780.18447583249104452No Hit
TCAGAGTTCTACAGTCCGACGATC440150.18338009320866627Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC407670.16984792138674765Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC401780.16739396535130735No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC395820.16491084515245774Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG389590.16231523460902939No Hit
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC383070.15959880110290534Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC382730.15945714659491725No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC368440.1535034909503601Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCC361600.15065373555436493Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC337350.14055043608756915No Hit
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC327760.13655494570049403No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC320490.13352603901498453No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC317860.13243029973260628Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC315980.13164703362961344No Hit
TACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC310450.12934306472027818Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTCTACAGTCCGACGATC306710.12778486513240947Illumina DpnII expression Sequencing Primer (95% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG305550.12730157328162667No Hit
CAGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATC301740.12571420953034865Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC300080.12502260222664222No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG294510.12270196808107305No Hit
GTTCTACAGTCCGACGATCC290700.12111460432979503No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG287770.1198938757756626No Hit
AGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC287600.11982304852166856Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCAGAGTTCTACAGTCCGACGATC284580.11856482318600987Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTT283460.11809819657146094No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC280160.11672331458216503Illumina Small RNA Adapter 1 (96% over 26bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC277990.11581922551647651No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC275400.11474015147033213No Hit
GTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC271560.1131402887918787Illumina Small RNA Adapter 1 (96% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC262060.10918229518632984No Hit
CAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC260310.10845319110109716Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC258590.10773658594303989No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA249650.10401190564476549No Hit
CCCCCGCGGGGGCGCGCCGGC247940.1032994667957667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)