FastQCFastQC Report
Tue 5 Sept 2023
SRR15324726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26190794
Total Bases838.5 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC11181834.269374193084792No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC10042713.8344427435075086No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC9198833.5122379260437846No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6820532.604170763207866No Hit
CAGAGTTCTACAGTCCGACGATC6770992.585255720006045Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC5509542.103617019018209No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC5225831.9952926971209806No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG5180521.9779927252300944No Hit
AGAGTTCTACAGTCCGACGATC5160111.9701999107014472Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC4576951.747541521650699No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG4204271.6052472483270266No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC4198151.6029105494090787No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA4080751.5580856387935393No Hit
AGTTCTACAGTCCGACGATC3462021.32184614181609No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3020661.1533289139687783No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2960651.1304162829122324No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2870161.0958659748917883No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2670341.0195719915936874No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC2548200.9729372847573846No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC2395410.9145999926539073No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG2183760.8337891550748708No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC2141600.8176918958623399No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2110420.8057869494143629No Hit
GAGTTCTACAGTCCGACGATC2023470.7725882613562612Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT1907900.7284620695348144No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1862350.7110704623922436No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1680060.6414696706025789No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC1588880.6066559112335426No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1521750.5810247677103642No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1497790.5718765150838878No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1492230.569753631753203No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1457660.5565543373751861No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1425770.5443783033076431No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1233250.4708715589149378No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1192360.455259202909236No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1172830.44780238430343117No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1140020.43527508177109864No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT1095250.41818128919650166No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC1065510.40682615425863No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1017120.3883501966377957No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1002860.3829055354335573No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC947410.36173397415901176No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC908850.34701124372174436No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC902050.344414911590691No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGG877630.3350910247318199No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG841150.32116246647581587No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC833940.31840959078980197No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC801790.30613428519960106No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC754020.2878950519789511No Hit
CCCCCGCGGGGGCGCGCCGGC739770.2824542089101995No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT672200.2566550674256No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT657360.2509889543631247No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT655240.2501795096399139No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG641090.2447768479260308No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG626410.239171825031345No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG624620.23848837877920004No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC617960.23594550054496247No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG588860.22483472627824874No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG581910.22218112211489274No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA574040.21917624948674716No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG558650.21330013897249545No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC549720.20989054398274448No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT544360.20784402336179653No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA525990.20083010847246555No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA520320.19866522565142544No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC517780.1976954192377673No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG509040.19435836882226634No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG497320.1898835140316861No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT493750.1885204396628831No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT489930.18706191190690896No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC485650.18542774991854008No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC456060.17412988701297105No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG441790.1686814076732458No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC435780.16638670824565305No Hit
ACAGAGTTCTACAGTCCGACGATC420100.16039987180228288Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG413400.15784172102609795No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC407490.1555852029533736No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT395090.15085071494968805No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT383080.14626513422998935No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG376670.14381770938292288No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT376640.14380625497646232No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG368580.14072883777406672No Hit
CAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC366820.14005684592838233No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA366710.14001484643802706No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT363340.13872813477896087No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC358490.13687633906784194No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT350220.1337187410202226No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA344840.13166458412830095No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT342790.1308818663534981No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC340300.12993115061727414No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT339320.1295569733395635No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT335060.12793044762216832No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC332700.12702936764727332No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT330950.12636119393707576No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT330480.12618174156919412No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG329750.12590301767865456No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC329570.1258342912398914No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTAT329250.12571211090431242No Hit
TAATCCCAGGGTCGTGGGTTCGGGCCCCACGTTGGG325530.12429176450320673No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT322400.12309668809582482No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGG318390.12156561576560068No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC312460.11930146142190268No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC290510.11092065402828184No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC289110.11038611506012379No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC282490.10785850936783359No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC279990.1069039754961228No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT278640.10638852720539897No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG268710.10259711866696367No Hit
CGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGT266410.10171894750498973No Hit
GTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG264980.10117295413037115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)