FastQCFastQC Report
Tue 5 Sept 2023
SRR15324727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19272578
Total Bases598.6 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8446324.382558472457602No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6672723.4622871937526987No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC6266233.2513709375050914No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC6062233.1455210610640676No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5820773.0202342416255883No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5478902.8428474903565055No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC5173952.6846174912354743No Hit
CAGAGTTCTACAGTCCGACGATC4210962.184948998520073Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC3209961.6655581832383815Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2803401.4546056059547405No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC2748421.4260780265099977No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2718481.4105430005264474No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2172731.127368637449541No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2099931.0895947599745088No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2074931.07662296139105No Hit
AGTTCTACAGTCCGACGATC2054741.0661469368550487No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1946381.0099219730749045No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1819300.9439837265154667No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC1761460.9139721733127764No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1697150.8806035186366868No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1626280.8438310640122977No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1571220.815261974812088No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1515980.7865994886620773No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1464430.7598516399829851No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1361350.7063663200636677No Hit
GAGTTCTACAGTCCGACGATC1226150.6362148333243223Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1175750.6100636873800692No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1142150.5926295900839006No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG940410.48795236423482113No Hit
CCCCCGCGGGGGCGCGCCGGC934810.4850466813521263No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC889310.46143800793023126No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC830780.4310684330866374No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG829720.4305184288266988No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC810900.4207532588530709No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC794910.41245649647909066No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG726430.3769241457992802No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGG713420.37017362181644825No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT687300.3566206866564504No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC680840.3532687739024847No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG635580.3297846297469908No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT619790.32159164176167815No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT615710.3194746442328577No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT572020.296805129028405No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA568700.29508247417652167No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC565460.2934013290801054No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT545100.28283709631373655No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG530130.2750695833219614No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA529930.2749658089332937No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGG516060.26776905507919074No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC506210.262658166437308No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT500800.2598510692238475No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG482100.25014816388342026No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC464210.24086554481709713No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGG444160.23046216235316314No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT439630.2281116724498404No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC429240.2227205929585549No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT418160.21697149182636594No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG415320.21549789550728504No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC404960.21012238217429968No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG390360.20254685180155968No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG367750.19081515716267952No Hit
AACGATGCCGACCGGCGATGCGGCGGCG362200.18793541787715168No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGG351320.18229009113363037No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC350620.18192688077329355No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG340060.1764475930516405No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG339660.17624004427430517No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTC333970.17328766291670994No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCG325690.1689914032258684No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG325280.16877866572909966No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGG305450.1584894350927001No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC304470.1579809405882285No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC303460.1574568799254568No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG290660.15081531905072584No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT287340.1490926641988425No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT278560.14453696853633177No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC265620.1378227655895335No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT264350.13716379822149377No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC260310.1350675555704068No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG254000.1317934736079418No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG250520.12998779924512435No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT245270.127263721542598No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC244110.1266618300883255No Hit
TCTTGCGACCCGGGTTTCCCGGGCGGCG243590.12639201667778954No Hit
CGGTCGGCGGGCGGCGGGGCGGGG237240.12309717983759101No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC231180.11995281586096057No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT231130.11992687226379367No Hit
CGGGTCGGGGCGGCGGCGGCGG230530.11961554909779065No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCC223880.11616505067459061No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC220090.11419852600933825No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC218770.11351361504413163No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCG216360.11226313366068617No Hit
CGGGTCGGGGCGGCGGCGGCGGC214530.111313598004377No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT212950.1104937803339024No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT210300.10911876968405576No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC208840.10836121664678176No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG207270.10754658769574055No Hit
CCCCCGCGGGGGCGCGCCGG202140.1048847746264148No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTC198680.10308947770246409No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT193870.10059370365500661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)