FastQCFastQC Report
Tue 5 Sept 2023
SRR15324728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23651488
Total Bases728.5 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC11871075.019164121936007Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC8267523.4955601947750603Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6712582.838121643762963No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5265842.226430742962134No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4770502.016997831172398No Hit
AGTTCTACAGTCCGACGATC4279971.8095986180658064No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3936881.6645379774837No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3509641.4838981801060465No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3319221.4033873894107636No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2865241.2114417494577931No Hit
GAGTTCTACAGTCCGACGATC2662501.125721984172835Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC2509471.0610199239895604No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2355180.9957851277687052No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2263910.9571955895544499No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2242290.9480545156397772No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2201280.9307152260356727No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1909490.8073445526979106No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1547260.6541913980211309No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC1513930.6400992614079926No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1450310.6132003195739736No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1444430.6107142180652652No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1338600.5659686189723031No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1291310.5459741053078775No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1242370.5252819611180489No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1228660.5194852856615194No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1172630.4957954442443536No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1136470.4805067655785547No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC1102310.4660636996708199No Hit
CCCCCGCGGGGGCGCGCCGGC1083030.4579119926830819No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1060740.4484876384944575No Hit
ACAGAGTTCTACAGTCCGACGATC1042780.4408940359270419Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1029750.4353848688082543No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1012710.42818024810954813No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC958240.4051499846436723No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC958200.40513307238851104No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC951190.40216919967149634No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG910700.3850497693844886No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG891930.37711369365005704No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC873290.36923258274489956No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT829470.35070520721571513No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG746760.31573489160597423No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC681580.28817637182066513No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC680130.2875633025710687No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC675490.28560148097236No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT664070.28077303212381394No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC641700.27131485342486694No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT639580.27041850390131905No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC614780.25993290570132416No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG612530.258981591348502No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT602930.25492265010979437No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC596820.25233930313390857No Hit
AAGAGTTCTACAGTCCGACGATC556360.23523255703827178Illumina DpnII expression Sequencing Primer (95% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT523170.221199613318198No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA517550.2188234414680379No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC515080.2177791097118287No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA506030.21395271198158863No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG494910.20925110504675226No Hit
CAGAGTTCTACAGTCCGACGAT482820.20413937592425477Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG473890.20036371495949853No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC456390.19296460332643764No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG426210.18020430680725036No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC417940.1767076980526553No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC404690.17110551353048062No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC389940.16486911943975785No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC386310.1633343322838715No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG378000.15982081127411518No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT375660.1588314443471802No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT374620.15839172571298687No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG373720.158011199971858No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT370150.15650178119871358No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT365240.15442580187766622No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG364310.1540325919451664No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC358970.15177480588113526No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT354360.1498256684737975No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG350300.14810907457492736No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC338140.14296774900589762No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT337870.142853591283559No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG334180.14129343574493072No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC333110.14084103291936642No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT329620.13936543865654458No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT319750.1351923396954982No Hit
AAGTTCTACAGTCCGACGATC318240.13455390206315984Illumina DpnII expression Sequencing Primer (95% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC313550.1325709401454995No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT312350.13206357249066106No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC312220.1320086076613869No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC309400.1308162936725165No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA309380.13080783754493586No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT302780.12801731544332431No Hit
AGAGTTCTACAGTCCGACGAT301880.12763678970219547Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT294140.1243642683284874No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG289990.12260962185550439No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT280500.11859718931849024No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC276320.11682985865413627No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG269050.11375605627857327No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG264700.11191684852978384No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC263590.11144753344905825No Hit
CCAGAGTTCTACAGTCCGACGATC262480.11097821836833269Illumina DpnII expression Sequencing Primer (95% over 24bp)
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC261610.1106103768185748No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC258160.10915169481091422No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG252560.10678397908833474No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGG250190.10578192797002879No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC246110.10405687794357801No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC245720.10389198345575551No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG242440.10250517853253038No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC240490.1016807060934179No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG240450.1016637938382566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)