FastQCFastQC Report
Tue 5 Sept 2023
SRR15324729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30279239
Total Bases931.3 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC16714495.520115614530471Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC13495474.457004352057857Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC9102213.0060894198827124No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG8777922.898989634448871No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC8419772.780707269426421No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC8287752.7371064378467374No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC7501522.477446675591814No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6832222.2564041322174577No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5487341.8122450171221278No Hit
GAGTTCTACAGTCCGACGATC5249661.7337489888698987Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC4791121.5823118936377496No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG4459801.472890385389144No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3669381.2118468367055064No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3660201.2088150564153874No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG3510321.159315793900897No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA3251581.0738645049831008No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC3092291.0212575025415929No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2812810.928956635931306No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC2623640.8664814858788228No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG2463190.8134913826599142No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC2337360.7719348560906699No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2031710.670991103838508No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2003600.6617075151723596No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1861410.6147479466046025No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1841460.6081592737518932No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1617250.5341118381475836No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1549520.5117433763774578No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1368160.4518475513866118No Hit
CAGAGTTCTACAGTCCGACGA1334690.4407937729214397Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1196160.39504295335823997No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1180650.3899206317569606No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1150290.37989395968637124No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1111820.367188884766886No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1067970.3527070148625598No Hit
AGAGTTCTACAGTCCGACGA1040600.3436678180716497No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1025900.33881300649596907No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1018720.3364417447875754No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG987600.3261640756559304No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC968630.3198990569082664No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC943740.3116789031586956No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG891660.29447899929057003No Hit
ACAGAGTTCTACAGTCCGACGATC879290.29039369186259933Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT873110.28835268944506826No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT864700.28557520880891357No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT848790.2803207834912892No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC820980.27113627261239953No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG819910.27078289517117654No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC782240.25834202768438136No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG767610.2535103342590611No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC749240.2474434710859147No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA714690.2360330125866109No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG678190.22397854847012502No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT673330.22237348831653267No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT663190.21902465910718563No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG654430.21613158771922902No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC652540.21550739765949864No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGT648230.2140839801158807No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC624200.2061478493564518No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT603900.19944358575194046No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC591100.19521626682889884No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC581810.19214815801678503No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC571310.18868043546272745No Hit
CCCCCGCGGGGGCGCGCCGGC570490.18840962284422008No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC561580.1854670125626341No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA545630.18019937687337517No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG530320.17514310713026837No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG522230.17247130946718972No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG507730.1676825497496816No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT505470.16693616375233208No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG504190.16651343186002793No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC478590.15805879401394465No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG462300.15267887016579249No Hit
AAGAGTTCTACAGTCCGACGATC459570.1517772623017375Illumina DpnII expression Sequencing Primer (95% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC456710.1508327207298704No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC453310.14970983914093747No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG450280.14870915348962369No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG444420.14677383404516872No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG427850.14130143759557498No Hit
CAGAGTTCTACAGTCCGACGATCC420830.13898301737371935Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGG402680.13298881124456266No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA391810.12939889275288588No Hit
AAGTTCTACAGTCCGACGATC385570.12733807477790313Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGAGTTCTACAGTCCGACGATCC384000.12681956769124877Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCAGAGTTCTACAGTCCGACGATC380350.1256141212796002Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTTCTACAGTCCGACGATCC368330.1216444046034314No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC365080.12057106190812787No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT362840.11983128109659558No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT354640.11712315491152206No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT354270.11700095897390288No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG344740.11385358793198205No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT339820.11222871222093792No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC333920.11028018240484842No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC330970.10930591749680367No Hit
CAGAGTTCTACAGTCCGACGAT330870.10927289156771741Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT326330.10777351438720106No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG323180.1067331976209838No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC315010.10403497921463614No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT314890.10399534809973263No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC314770.1039557169848291No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGG311050.10272715242282014No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG308190.10178261085095303No Hit
AACGATGCCGACCGGCGATGCGGCGGCG303840.1003459829357006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)