FastQCFastQC Report
Tue 5 Sept 2023
SRR15324730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12797899
Total Bases404.4 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4862703.7996080450392675No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4331753.384735260061046No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3827632.9908268536890312No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC3675372.87185420044337No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3641302.845232643264336No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2078591.624165028962957No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2017881.5767275550463402No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1970051.5393542330659118No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1899811.4844702243704222No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1805901.4110909923574173No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1645791.2859845198028208No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1601701.2515335524995157No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1590871.243071225987953No Hit
ACAGTCCGACGATCCAGAGTT1503021.1744271462057951No Hit
TACAGTCCGACGATCCAGAGTT1415191.1057986939887556No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1408371.1004696942834131No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1401041.0947421916675542No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1395821.0906633971716764No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1320841.032075655543148No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1319871.0313177186349103No Hit
CAGTCCGACGATCCAGAGTT1250440.9770666263267119No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1014120.7924113168888112No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1005120.7853789125855736No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC992500.7755179189959227No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG988530.7724158473199391No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC962180.7518265302765712No Hit
ACAGTCCGACGATCAGAGTT916340.7160081510254144No Hit
TACAGTCCGACGATCAGAGTT865940.6766266869272839No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG799430.6246572191263582No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC722080.5642176110313107No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG658330.5144047472167111No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT621420.4855640757908779No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA611650.4779300102305855No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC579880.4531056230401568No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC553880.43278978838635934No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC551180.43068006709538803No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC493960.3859696032919153No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC493360.38550077633836616No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC489770.3826956283996303No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG478970.3742567432357452No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT448920.3507763266454908No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG446790.3491119909603912No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC424150.33142158724646914No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG413440.32305302612561637No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT399300.3120043375869742No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT399110.31185587571835033No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC397810.3108400839856605No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT390450.30508914002212395No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC381810.2983380318910159No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT374430.29257146036236104No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT350610.2739590303064589No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA344300.2690285335116334No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG340830.2663171509636074No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG336940.26327758954809694No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC319030.24928310498465414No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT316330.24717338369368283No Hit
CCCCCGCGGGGGCGCGCCGGC314060.24539965505275516No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG309220.24161778429412514No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC289720.22638090830377705No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC287050.22429462836048322No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT275230.21505873737556452No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA271380.21205043109029068No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC266450.20819823628862832No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC266260.2080497744200044No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG251090.19619626627776948No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG242740.18967175784087686No Hit
TACAGTCCGACGATCGAGTT235110.1837098417482432No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC224720.17559132166928337No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT224410.17534909440994964No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC211900.1655740524284494No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA211150.16498801873651292No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG208520.16293299392345573No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT206680.16149525793257158No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA205880.16087015532783935No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG199450.15584589314230407No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC198910.1554239488841098No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT197290.15415811610952704No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC189040.1477117454982259No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGG186630.14582862390147008No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGC184780.14438307412802678No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG174190.13610827839788392No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT174120.13605358191996983No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA168100.1313496848193598No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC163280.12758344162584812No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGG161020.1258175267674796No Hit
CAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC155890.12180905631463414No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGG151200.11814439229439146No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG147300.11509701709632182No Hit
GCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCG147250.11505794818352606No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT142620.11144016685863828No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTG141790.11079162290622858No Hit
TAATCCCAGGGTCGTGGGTTCGTGCCCCACGTTGGG137390.10735355858020132No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG136210.10643153223822129No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTC135920.10620493254400586No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC135870.1061658636312101No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG133980.10468905872753019No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC133390.10422804555654018No Hit
CCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCC130840.10223553100395619No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGT129420.1011259738805565No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC129230.10097751201193259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)