FastQCFastQC Report
Tue 5 Sept 2023
SRR15324731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36562797
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length20-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC22964436.280818724016108Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC17377934.7528995114897805Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC15485844.2354090142501954No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC12082883.304692471968159No Hit
AGTTCTACAGTCCGACGATC9893802.705974600356751No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG8605562.353638317112337No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC7898012.1601219403428025No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC7272091.9889315360638302No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC7141421.9531930229517178No Hit
GAGTTCTACAGTCCGACGATC6057861.6568371396750639Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG5476641.4978722771127166No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC4941351.3514693637907407No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC4936051.3500198029160626No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT3651970.9988212882072451No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3453370.9445037807145882No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC3224690.8819593315030029No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3169430.8668456081190944No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG3087160.8443445943153638No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC3084560.843633488980616No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG2924870.7999579463245112No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2640030.722053621882374No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG2610520.7139825763329868No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC2207500.603755779406045No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC2190480.5991007744839653No Hit
CAGAGTTCTACAGTCCGACGA2094350.5728090222419253Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCCCCGCGGGGGCGCGCCGGC2036940.5571072694465907No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC2005990.548642380942574No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1964090.5371826449710617No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT1796090.4912343002642823No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1779740.48676254171692607No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC1746270.47760842804230763No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1725640.4719660807131358No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC1709280.4674915871452613No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1578880.4318269195871421No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1575890.43100914845218213No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1562190.42726217034216496No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT1498960.40996863560520275No Hit
AGAGTTCTACAGTCCGACGA1487710.4068917375221595No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC1487510.40683703711179425No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1314260.359452806632928No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC1300950.355812494323123No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1243000.3399630504198024No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1222750.3344246338703245No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC1173160.3208616671202698No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC1080430.29549982185443857No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC1067620.2919962605705466No Hit
ACAGAGTTCTACAGTCCGACGATC1009200.2760182707028677Illumina DpnII expression Sequencing Primer (95% over 24bp)
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC926420.25337777085270585No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA914300.2500629259845739No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT865760.23678713638893653No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT862030.2357669737356253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC812240.2221493065752054No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC729410.1994951316224522No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT725210.1983464230047827No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG701640.1918999796432423No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC681540.1864025884015383No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC667560.1825790297170099No Hit
CAGAGTTCTACAGTCCGACGAT665150.18191988977210904Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC658890.18020776692767787No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC645060.17642523355092338No Hit
CAGAGTTCTACAGTCCGACGATCC626970.1714775814333898Illumina DpnII expression Sequencing Primer (100% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT622480.17024955722069077No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG611480.167241034650604No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG604200.16524993971331023No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT545750.14926374478407656No Hit
AGAGTTCTACAGTCCGACGATCC539270.14749145148824364Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAT538180.14719333425175324No Hit
CCGCCGAGGGCGCACCACCGGCCCGTC497680.13611650115279747No Hit
CCGGCCGAGGTGGGATCCCGAGGCCT485660.13282900648984813No Hit
AAGAGTTCTACAGTCCGACGATC483120.13213431127820993Illumina DpnII expression Sequencing Primer (95% over 23bp)
CCGGCCGAGGTGGGATCCCGAGGCCTCT478750.13093910731173No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT465870.12741640088421025No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG463070.12665059513909727No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC461100.1261117960969999No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC460780.12602427544041556No Hit
AGAGTTCTACAGTCCGACGAT456850.12494941237673912Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGGCCGAGGTGGGATCCCGAGGCCTCTC449900.12304857311654795No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGG432800.11837168803032218No Hit
TCAGAGTTCTACAGTCCGACGATC424290.11604418556928235Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGGCCGAGGTGGGATCCCGAGGCCTCTC420410.11498299760819719No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG418620.11449342893542855No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG417980.11431838762225988No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA417770.11426095219137639No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG414320.11331737011257645No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC412970.11294814234261126No Hit
AACGATGCCGACCGGCGATGCGGCGGCG410630.11230814754133826No Hit
GTTCTACAGTCCGACGATCC397130.10861586984168635No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC392750.10741793085468816No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTC387490.10597931006208305No Hit
CCAGAGTTCTACAGTCCGACGATC373220.10207643578252507Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGT366220.10016192141974259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)