FastQCFastQC Report
Tue 5 Sept 2023
SRR15324732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32060016
Total Bases939.2 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC26591818.294384506857389Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC19246696.003331377002432Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC9958623.1062429912698737No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC8344212.6026842906129555No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6983792.1783488816724232No Hit
GAGTTCTACAGTCCGACGATC6154521.9196871267936986Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5752831.7943939890734926No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC5085871.586359158398424No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG2821570.8800900161746644No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2716310.8472578429156118No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC2492230.777363928951252No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG2414660.7531686821366527No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT2382220.7430501594259966No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA2338510.7294163546268972No Hit
CAGAGTTCTACAGTCCGACGA2258600.7044912267043161Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2221810.6930158737288217No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2179760.6798998478353847No Hit
ACAGAGTTCTACAGTCCGACGATC2077870.6481188281378275Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC2032330.6339142188824859No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1898190.5920739403249207No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1713070.5343322348934574No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1632970.509347843120228No Hit
AGAGTTCTACAGTCCGACGA1595110.4975387410910837No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1526170.47603532075592225No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1458060.4547907898735921No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1433000.4469741998881099No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1397140.4357889278657877No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1349430.42090746305304405No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1284920.40078582618299385No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC1130310.35256064750560323No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1127210.35159371099502884No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1082130.3375325826412563No Hit
AAGAGTTCTACAGTCCGACGATC1017860.3174858053720248Illumina DpnII expression Sequencing Primer (95% over 23bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT1006470.3139330934831723No Hit
CCCCCGCGGGGGCGCGCCGGC945950.2950559974767324No Hit
CAGAGTTCTACAGTCCGACGAT910610.28403292125618407Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC855950.26698364716973316No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT851510.2655987445545879No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT834130.2601776617953029No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG776370.24216145119827764No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTC771490.24063930598163144No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA769010.2398657567731719No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC738570.23037106406933794No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC732950.22861810175016756No Hit
CAGAGTTCTACAGTCCGACGATCC718120.2239924022495809Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG716940.2236243425455558No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC702550.21913588564647005No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT698450.21785703413248456No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC686710.2141951519924382No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT681840.2126761259258261No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC647230.20188074765776784No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC631250.19689634590325844No Hit
AAGTTCTACAGTCCGACGATC619410.19320327226287098Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGAGTTCTACAGTCCGACGAT614130.19155636104486037Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC608230.18971606252473486No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC597570.1863910485883725No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT595210.18565492918032228No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC585070.18249211104573373No Hit
AGAGTTCTACAGTCCGACGATCC575320.17945093976247548Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTC556170.17347776744715285No Hit
CCAGAGTTCTACAGTCCGACGATC555320.17321263969425343Illumina DpnII expression Sequencing Primer (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC504820.15746093202199274No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC501550.15644096996083845No Hit
AACGATGCCGACCGGCGATGCGGCGGCG491890.15342787102788719No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG471300.1470055411076526No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG456190.14229250540611085No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT455920.14220828835518984No Hit
TCAGAGTTCTACAGTCCGACGATC445150.13884896376845227Illumina DpnII expression Sequencing Primer (100% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAT436880.13626942669024245No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG430570.1343012430187184No Hit
GCCGGCCGAGGTGGGATCCCGAGGCC428790.13374603431264662No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC425820.13281964675251567No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC423130.13198059539333978No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC411910.12848090905506723No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT409090.12760130874544792No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG403560.1258764187765845No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC383560.11963811870836247Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTCTACAGTCCGACGATCC370280.11549588746306302No Hit
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC368820.11504049155808282Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGT358150.11171235847168635No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT354960.11071734961080493No Hit
CAGTTCTACAGTCCGACGATC344700.10751710167580701Illumina DpnII expression Sequencing Primer (95% over 21bp)
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC340110.10608541181015005Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC335000.10449152614271932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)