FastQCFastQC Report
Tue 5 Sept 2023
SRR15324733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22200778
Total Bases646.4 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC274176512.349859991393094Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC17952028.086212113827724Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC10163134.577826056366133No Hit
GAGTTCTACAGTCCGACGATC5990842.698482008153048Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4995782.2502724904505595No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4218891.9003343035996305No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4208331.895577713537787No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3220381.4505707863030746No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3178011.4314858695492563No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2072370.933467286596893No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC2030220.9144814654693632No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2010770.9057205112361377No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1818350.819047873006973No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1757870.7918055844709585No Hit
ACAGAGTTCTACAGTCCGACGATC1705650.7682838862674092Illumina DpnII expression Sequencing Primer (95% over 24bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTA1393670.627757279497142No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1336310.6019203471157633No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1272250.5730655024792374No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1243190.5599758711158681No Hit
CAGAGTTCTACAGTCCGACGAT1122730.505716511376313Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1053910.47471759773463795No Hit
CAGAGTTCTACAGTCCACGATC1039280.4681277385864585No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1032600.46511883502461043No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC995590.4484482480749098No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG984430.4434213972140976No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC804260.36226658363053765No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG798820.3598162190532242No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC787440.35469027256612357No Hit
AAGAGTTCTACAGTCCGACGATC742590.3344882778432359Illumina DpnII expression Sequencing Primer (95% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG674700.3039082684399619No Hit
AGAGTTCTACAGTCCGACGAT664410.2992732957376539Illumina DpnII expression Sequencing Primer (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTT663300.29877331325956236No Hit
CCAGAGTTCTACAGTCCGACGATC662390.29836341771446023Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG608100.27390931975446986No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC581530.2619412707068194No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC552900.2490453262493774No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG549850.24767150052128806No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG531860.23956818089888562No Hit
AGAGTTCTACAGTCCACGATC525110.23652774691049114No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT521380.23484762561023761No Hit
AAGTTCTACAGTCCGACGATC512480.23083875709220641Illumina DpnII expression Sequencing Primer (95% over 21bp)
TCAGAGTTCTACAGTCCGACGATC486930.21933015140280218Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGAGTTCTACAGTCCGACGATCC483270.21768156052909496Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTTA478410.215492448057451No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC472850.21298803132034383Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC469100.21129890132679133No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG445430.20063711280748808No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG435180.196020157491778No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC424150.19105186313740896No Hit
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC420590.1894483157301965Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC417830.18820511605494186Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC413180.1861105948629368No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT411520.18536287331912424No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC398440.17947118790161318Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC395860.17830906646604908No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC385260.1735344590176074No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC375220.16901209498153622Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCC370400.1668409998964901Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC367160.16538159158206078No Hit
CAGTTCTACAGTCCGACGATC357970.16124209701119482Illumina DpnII expression Sequencing Primer (95% over 21bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG349760.1575440284119773No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT348370.15691792422770048No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT343920.1549134899686849No Hit
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC340640.15343606426765766Illumina Small RNA Adapter 1 (96% over 26bp)
TACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC329560.14844524818004126Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC329060.1482200308475676Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC322910.14544985765814153Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT319600.1439589189171659No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT316200.14242744105634497No Hit
GTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC308090.13877441592362214Illumina Small RNA Adapter 1 (96% over 26bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT302310.1361709035602266No Hit
CAGAGTTCTACAGTCCCACGATC300200.1352204864171877Illumina DpnII expression Sequencing Primer (95% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTT300010.13513490383084772No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC298050.13425205188755096No Hit
CAGAGTTCTACAGTCCGACGATCTCTACAGTCCGACGATC289810.130540470248385Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC283840.12785137529864943Illumina DpnII expression Sequencing Primer (100% over 22bp)
TTCAGAGTTCTACAGTCCGACGATC280260.126238819198138Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTTCTACAGTCCGACGATCC279400.1258514453862833No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG274270.12354071555510351No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC272200.12260831579866255No Hit
CAGAGTTCTACAGTCCGACGATCAGTTCTACAGTCCGACGATC272180.12259930710536361Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC267570.12052280329995643Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC265620.11964445570330914No Hit
CAGAGTTCTACAGTCCGACGATCA265530.11960391658346389Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC259140.11672563907445044Illumina Small RNA Adapter 1 (96% over 26bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTC257810.11612656097007051No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC257440.11595990014403999No Hit
TCTACAGTCCGACGATCGTTCTACAGTCCGACGATC245190.11044207549843524No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC244610.11018082339276578No Hit
CTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC242200.10909527585024272Illumina Small RNA Adapter 1 (96% over 26bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC240020.10811332828065755No Hit
ACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC232810.10486569434638732Illumina Small RNA Adapter 1 (96% over 26bp)
GATCCAGAGTTCTACAGTCCGACGATC223490.10066764326907823Illumina DpnII expression Sequencing Primer (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)