FastQCFastQC Report
Tue 5 Sept 2023
SRR15324734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19781390
Total Bases598.2 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC19120479.66588798866005Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC13214376.680202958437198Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC6609683.3413627657105995No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6284503.176975935462574No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5906282.985776024839508No Hit
GAGTTCTACAGTCCGACGATC4141622.093695134669505Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3743971.8926728607039243No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG3320801.6787495721989205No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG3318891.6777840182110557No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3094911.5645563835503977No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC2836491.4339184455692953No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2698481.3641508508754947No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2192961.1085975252497424No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2182291.1032035665845525No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1808260.9141218084270115No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1753840.8866111026575989No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1597410.8075317255258605No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1447580.731788817671559No Hit
ACAGAGTTCTACAGTCCGACGATC1354780.684876037528202Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1332630.6736786444228642No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1138140.5753589611245722No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1137070.5748180486811089No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1063010.5373788191830806No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG926570.4684048997567916No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC913540.46181790056209393No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC906790.4584056024374425No Hit
CAGAGTTCTACAGTCCGACGAT873750.4417030350243335Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC753170.38074675237685524No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC724270.3661370611468658No Hit
AAGAGTTCTACAGTCCGACGATC706120.356961770633914Illumina DpnII expression Sequencing Primer (95% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC704920.3563551398561982No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA675470.3414674095197557No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC650200.32869277639235667No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC642600.3248507814668231No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG624660.31578165133997155No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT603340.30500384452255375No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC602600.30462975554296234No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT595180.30087875523408614No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC569330.28781091723079116No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG558280.28222485881932463No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT511110.25837921399861186No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC506860.25623072999420166No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC488250.24682289768312543No Hit
AGAGTTCTACAGTCCGACGAT486390.24588261997766586Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT484480.2449170659898015No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG470490.2378447621729312No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT469890.23754144678407332No Hit
CCAGAGTTCTACAGTCCGACGATC462770.2339421041696261Illumina DpnII expression Sequencing Primer (95% over 24bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC452990.22899806333124212No Hit
AAGTTCTACAGTCCGACGATC438360.22160222309959005Illumina DpnII expression Sequencing Primer (95% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC436920.2208742661663311No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG425640.21517193685580233No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG415330.20995996742392722No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG407320.20591070698267414No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC386760.1955170996578097No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC385130.19469309285141237No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG364130.18407705424138546No Hit
TCAGAGTTCTACAGTCCGACGATC344830.17432040923312264Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC340960.17236402497498912No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG333920.16880512441238962No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG331580.16762219439584378No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC311710.15757739976816595No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC308800.15610632013220507No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG304250.15380617843336591No Hit
CCCCCGCGGGGGCGCGCCGGC291810.14751743937104522No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA291240.1472292897516302No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC288430.145808762680479No Hit
CAGAGTTCTACAGTCCGACGATCC287920.14555094459994974Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCA280300.1416988391614543Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTT277560.1403136988856698No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT277210.14013676490883603No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC272290.13764957872020117Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCT264240.13358009725302417No Hit
CAGTTCTACAGTCCGACGATC262230.1325639907003502Illumina DpnII expression Sequencing Primer (95% over 21bp)
CAGAGTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC254200.12850461974613514Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT251230.12700320857128847No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC246940.1248345035409544Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTC239820.1212351609265072No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC239410.12102789541078762No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG234890.11874291948139135No Hit
ACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC222440.11244912516258967Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCC222110.11228230169871783Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC221830.1121407545172508Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATCAGAGTTCTACAGTCCGACGATC217550.10997710474339771Illumina Small RNA Adapter 1 (96% over 26bp)
GTTCTACAGTCCGACGATCGTTCTACAGTCCGACGATC215570.1089761639601666No Hit
TTCAGAGTTCTACAGTCCGACGATC214260.10831392536116016Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG207410.104851074671699No Hit
GTTCTACAGTCCGACGATCA204080.1031676742635376No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC202720.10248015938212633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)