FastQCFastQC Report
Tue 5 Sept 2023
SRR15324736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19388632
Total Bases598 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC6129493.1613834333438278No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC4874862.514287753772417No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4618532.3820814176059453No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC4526032.3343730491145536No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC3802691.9612987651733242No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA3196421.648605223927093No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC3184661.6425398140518628No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG3026001.5607083573508436No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3005681.550227989267113No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2594661.338237787998658No Hit
ACAGTCCGACGATCCAGAGTT2185761.1273410109594115No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT2120181.0935170671143792No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1978751.020572261106405No Hit
CAGTCCGACGATCCAGAGTT1819350.9383591374574545No Hit
TACAGTCCGACGATCCAGAGTT1803200.9300295142019303No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC1780660.9184041452744062No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1764660.9101518869407599No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC1712080.8830329029918149No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC1649590.8508026765374679No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1646910.8494204232665823No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1561690.805466832316999No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT1550450.7996696208376125No Hit
ACAGTCCGACGATCAGAGTT1437110.741212685866646No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG1421130.7329707428559168No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1410200.7273334188817447No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA1264380.6521243994934764No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1213580.6259234792841496No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1203230.6205852996745722No Hit
TACAGTCCGACGATCAGAGTT1199000.6184036088776145No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCT1140040.5879940369181281No Hit
CCCCCGCGGGGGCGCGCCGGC1117710.5764769788812331No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1091230.5628194913390486No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG993420.5123724046131775No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG893950.46106914608519056No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC817290.421530513344108No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT785730.40525293378099086No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC765050.3945868898847531No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC755100.38945501673351685No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC746120.3848234367437579No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG704970.3635996598419115No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC677010.34917883840386466No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC665750.3433713116015612No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC654070.3373471630179994No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC610280.31476176349110135No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG601680.31032617463676654No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT597520.30818058747001853No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG583340.30086702352182454No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC573790.2959414568289295No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC571380.294698460417424No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT544720.28094813496898596No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT543900.2805252067293866No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG537910.27743576751572774No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCT535830.27636297393235376No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC518210.2672751744424258No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG515100.26567114172882333No Hit
AACGATGCCGACCGGCGATGCGGCGGCG510790.2634481896401974No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCT455090.2347200153161915No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGG445920.22999043975872047No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT423190.21826707526348427No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG420320.21678682642488648No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA410890.21192315166949374No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCC408560.21072141654965654No Hit
TAAGTGGGAGGCCCCCGGCGCCCCCCCGG399480.2060382599453123No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCC397500.20501704297652357No Hit
CGCGCGTCCGCTGGGGGCGGGGAGCGGT395990.2042382360962857No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATT392360.2023660049868397No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC384910.1985235472002357No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA377050.19446962529383197No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGA346750.17884191107448943No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC345160.17802184290258333No Hit
TACAGTCCGACGATCGAGTT335930.17326132137636116No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT335100.17283323547530327No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC326720.16851111517305603No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT324200.16721138448550676No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG311580.1607024157248433No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT309160.15945426165187931No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCT297840.1536157888808246No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGG292070.15063981821925343No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT288790.14894810526085595No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC282720.1458174047555289No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG273830.14123224371889673No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG273030.1408196308022144No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA270850.1396952606042551No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC265810.13709579922915655No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATT252770.13037020868723487No Hit
TTGCGACCCGGGTTCGTTTCCCGGGCGGCG241490.12455236656201427No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC224890.11599064854085632No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG223070.11505195415540406No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA222110.11455681865538529No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG222080.1145413456710097No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGC214910.11084330240524448No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATT213690.11021406770730395No Hit
GTGTTATCGCAAGGCATTTACGTATGAGTGTCAGC210470.10855330071765765No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC209620.10811489949368268No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG208370.10747019181136658No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG207240.10688737606655281No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCC205930.10621172241548553No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC205620.10605183491027112No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTC204120.10527818569149179No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG202740.1045664284102148No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC202630.104509694134171No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC201930.10414865783207397No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG200950.10364320700913814No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGT200630.10347816184246522No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGG199050.10266325133201765No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC198070.10215780050908181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)