FastQCFastQC Report
Tue 5 Sept 2023
SRR15324737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21467123
Total Bases657.8 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC20269179.441959222947574Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC13511116.293861548191623Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC7029973.274761131242412No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC6753153.145810456296356No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC5914752.7552597523198616No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG5161972.4045932936611956No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4559912.1241365226257845No Hit
GAGTTCTACAGTCCGACGATC4161331.9384665565106232Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC3822011.7804015936369304No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC3605001.679312127666106No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2803011.3057222432647355No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC2727961.270761806321229No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2568011.1962525206568202No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2499061.164133638215051No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2297701.0703343899413071No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2212711.030743616645789No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1998340.9308839381970281No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG1866140.8693013963724902No Hit
ACAGAGTTCTACAGTCCGACGATC1507490.7022319665285376Illumina DpnII expression Sequencing Primer (95% over 24bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1251820.5831335666171942No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1230840.5733604824456449No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC1219790.5682130763400387No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG1043310.4860036438045285No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC1009540.4702726117514676No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC984900.45879459487887597No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG844510.39339691676430044No Hit
AAGAGTTCTACAGTCCGACGATC834910.3889249621386154Illumina DpnII expression Sequencing Primer (95% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC788750.3674223136467798No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC720150.33546647121740536No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC706140.3289402124355462No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG703880.32788743978408286No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC698770.32550705560311927No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC692120.32240929536761864No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC679730.31663767892884387No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGC667810.31108500193528493No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG665760.3101300532912585No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG658400.3067015547448999No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT631300.2940775994994765No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG609250.2838060787186061No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA602180.28051267046823186No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC587210.27353921622380417No Hit
CAGAGTTCTACAGTCCGACGAT583280.27170850979891437Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTC576840.26870857357085065No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG549370.2559122617408956No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC544360.2535784604206162No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT524100.2441407728459934No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG517690.24115481147613493No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC512360.23867194500166605No Hit
AAGTTCTACAGTCCGACGATC497450.23172644047364893Illumina DpnII expression Sequencing Primer (95% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGTTAT480990.22405890160502642No Hit
CCAGAGTTCTACAGTCCGACGATC480810.22397505245579483Illumina DpnII expression Sequencing Primer (95% over 24bp)
AGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC476140.2217996328618418No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC469270.21859939033283593No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC468930.21844100860650958No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG466210.21717395479589882No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC461980.21520349978895637No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT448880.20910114503932364No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC447690.2085468089971814No Hit
CAGAGTTCTACAGTCCGACGATCA433720.202039183359596Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT420220.19575049716722637No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT419520.1954244171424368No Hit
GTTCTACAGTCCGACGATCA414700.19317912325745745No Hit
ACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTC412240.19203318488462567No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCC407840.18998353901452No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC403750.18807830001253545No Hit
TCAGAGTTCTACAGTCCGACGATC383050.17843564785090205Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC368740.17176964048699028No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCT362400.16881628711961078No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC356930.16626820464018396No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG345850.16110682367637247No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG345450.16092049223363558No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA334550.15584296041905568No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG334410.15577774441409778No Hit
CAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC331640.1544873991731449No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG327170.15240514530056032No Hit
AGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTC322600.1502763085672915No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTTA312840.14572982136451168No Hit
AGAGTTCTACAGTCCGACGAT305360.14224542338533208Illumina DpnII expression Sequencing Primer (100% over 21bp)
CAGAGTTCTACAGTCGACGATC300240.13986038091830003No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG296200.13797843334665758No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG295570.137684961324347No Hit
TTCAGAGTTCTACAGTCCGACGATC280870.13083728080376678Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCGTT255820.11916827420236982No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC251530.11716986947901682No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC250140.11652236771550618No Hit
AGAGTTCTACAGTCCGACGATCA244140.11372739607445302Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGC228090.10625084693463582No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG227770.10610178178044631No Hit
CAGTTCTACAGTCCGACGATC227650.10604588234762524Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCC217370.10125716426928752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)