FastQCFastQC Report
Tue 5 Sept 2023
SRR15324738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15324738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8635322
Total Bases256.6 Mbp
Sequences flagged as poor quality0
Sequence length20-44
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGTTCTACAGTCCGACGATC96123311.131408880873233Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAGTTCTACAGTCCGACGATC5300146.137744487119299Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTTCTACAGTCCGACGATC2336842.7061411259475907No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1975112.2872453395484267No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC1781712.0632814850447962No Hit
GAGTTCTACAGTCCGACGATC1577681.8270077247843217Illumina DpnII expression Sequencing Primer (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1488041.7232015204528564No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1288451.49206943296382No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1266711.4668937649343012No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1081801.252761622554434No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG885811.0257984589341314No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG885161.0250457365689434No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGC864091.0006459515927721No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC789760.914569254047504No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC741520.8587056742064743No Hit
ACAGAGTTCTACAGTCCGACGATC674430.7810131457749925Illumina DpnII expression Sequencing Primer (95% over 24bp)
CCGGCCGAGGTGGGATCCCGAGGCCTCTCC645630.7476617548251241No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCC643680.7454035877295601No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC564070.6532124685101494No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG539150.6243542510632494No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA531970.6160395640139418No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG524540.6074353683626389No Hit
CAGAGTTCTACAGTCCGACGAT521470.6038802027301355Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG514660.5959939884117813No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC472410.5470670346745611No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC443050.5130671444562229No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG404490.4684133376844546No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCG404370.46827437355549684No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG387130.448309860361895No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG335500.3885205438778079No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCG332160.3846527089551496No Hit
AAGAGTTCTACAGTCCGACGATC327460.3792099472376363Illumina DpnII expression Sequencing Primer (95% over 23bp)
CCAGAGTTCTACAGTCCGACGATC300300.34775773271685756Illumina DpnII expression Sequencing Primer (95% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG275490.3190268990548355No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT269210.3117544429727114No Hit
CAGAGTTCTACAGTCCGACGATCA252910.2928784821226122Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG237880.27547322497064963No Hit
CAGAGTTCTACAGTCCGACGA221540.25655094274423124Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT217870.25230095646693895No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCTC212360.2459201868789606No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTC212160.24568857999736432No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG205590.23808029393692556No Hit
TCAGAGTTCTACAGTCCGACGATC202670.23469883346561946Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC201100.23288071944508842No Hit
GTTCTACAGTCCGACGATCA198020.2293139734685053No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG197070.22821384078092283No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGG193770.22439232723458374No Hit
AGAGTTCTACAGTCCGACGAT191870.2221920618594188Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGTTCTACAGTCCGACGATC189980.22000337682833368Illumina DpnII expression Sequencing Primer (95% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGA186460.21592709571223867No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCC180650.20919891580186586No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGC180300.20879360375907233No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT172160.1993672036781026No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC160870.18629299521199094No Hit
TCTTGCGACCCGGGTTCGGTTCCCGGGCGGCG151020.17488635629337274No Hit
TTCAGAGTTCTACAGTCCGACGATC150950.17480529388481403Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCC149190.1727671533267665No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG146220.16932779113506133No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT140280.16244906675165094No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTTA139170.16116364855879142No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT136280.15781692911972478No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC134330.15555876202416077No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC124910.14465007790097464No Hit
AGAGTTCTACAGTCCGACGATCA123280.1427624818159647Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCGGCCGAGGTGGGATCCCGAGGCCT120600.13965894960257416No Hit
GATCCAGAGTTCTACAGTCCGACGATC119760.13868620069986967Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCGGCCGAGGTGGGATCCCGAGGCCTCT119510.13839669209787428No Hit
CGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT119160.13799138005508074No Hit
GCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGC119020.13782925523796333No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG118890.13767871076492574No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC117010.13550160607792042No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC116280.1346562409600939No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC115710.1339961613475444No Hit
AGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTG113570.13151796771446392No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC113150.13103159326311167No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC109830.12718691902861295No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTC108250.1253572246640021No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGC107380.12434973472905816No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC106340.12314537894475734No Hit
GCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCG104700.12124620251566763No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCG104570.12109565804263003No Hit
GTTCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC100650.11655616316334239Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC99300.11499281671256728Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCCCC97700.11313996165979681No Hit
ATCCAGAGTTCTACAGTCCGACGATC97060.11239881963868864Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGAGTTCTACAGTCCGACGATCC94110.10898261813514307Illumina DpnII expression Sequencing Primer (100% over 23bp)
CAGTTCTACAGTCCGACGATC93720.10853098471603029Illumina DpnII expression Sequencing Primer (95% over 21bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC93320.10806777095283765No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGG93130.10784774441532116No Hit
CCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGT92670.10731504858764965No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGC92020.10656232622246165No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG91590.1060643714270296No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCT90950.1053232294059214No Hit
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATC89310.10342405297683167Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTC88900.10294925886955925No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG87450.101270108977986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)