FastQCFastQC Report
Sat 16 Sept 2023
SRR15359342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120365429
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA110917849.21509115378968No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA57173104.749960223213261No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG55695264.627180782947236No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT30965542.5726273945320295No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA19148921.5908986624390296No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG16954121.4085539461667187No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG16709781.3882540974452058No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG13180651.0950527995874961No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT12647211.0507344264107594No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA10841000.9006738969874812No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAATAGATCGGAAGA9139320.7592977548395562No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT8955210.7440018346131595No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG8358880.6944585392538252No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA8109400.6737316576174044No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA7657670.6362017785023638No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT7615350.6326858187827338No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGA7053130.5859763936038478No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAATAG6325040.5254864334841527No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG5763500.4788335029321417No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCG5637730.46838448937028254No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG5410750.449526915240754No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT4710110.3913175102794674No Hit
ATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATCGG4595890.38182807457114615No Hit
TGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA4157900.3454397192403144No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC4009180.3330840120214252No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT3809680.3165094854603144No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA3606490.2996283924680732No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAATA3533190.29353860401228665No Hit
ATTAAATCAGTTATAGTTTATTTGATACTGTAGGCACCATCAATAGATCG3455870.2871148326152686No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG3061410.25434296420777097No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA2941130.2443500616775935No Hit
GATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATA2743090.22789683240359654No Hit
TCGCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAATAGA2711210.22524823136716438No Hit
GCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAATAGATC2677030.22240854556336104No Hit
GTCTAGTGGTATGATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2559610.21265325278739294No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA2556390.2123857341130733No Hit
ACCCCGGTCGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2528240.21004702272111705No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCTGTAGGCACCATCA2377870.19755423295172236No Hit
TTATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATC2322830.19298149138819587No Hit
AGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAATAGATCG2297380.19086709689706668No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG2285520.18988176414010038No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGA2155290.17906221229020836No Hit
CTGGAGATAGGGTTTAATGGCCGGCTGTAGGCACCATCAATAGATCGGAA2062580.1713598345584761No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGCTGTAGGCACCATCAATA1960940.1629155494473417No Hit
GCCTGGGTCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1942610.1613926869317269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAATAGATCGGAAG1914440.15905231393309785No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1887670.15682825340156434No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT1880300.15621595134264007No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGGCTGTAGGCACCATCAAT1869550.15532283775601383No Hit
TGATCCTTTAGTCCCTCGAAATTTGAGGCTAGAGGTCTGTAGGCACCATC1844140.15321176647823023No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGATCG1803800.1498603058192066No Hit
ACCAGCGGAGGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1738330.14442103637581852No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCTGTAGGCACCATC1707110.14182726835958853No Hit
TGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC1671840.13889702499211798No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1561090.12969587804152635No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC1497060.12437624427857935No Hit
GTCTAGTGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1469560.1220915351034889No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCCTGTAGGCACCAT1452630.12068498505496957No Hit
TGGTGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGAT1445310.12007683701272731No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGA1388530.11535953566866779No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAATAG1385490.11510697145440324No Hit
GTGTAGCGGTAACATCGTTGACCTGTAGGCACCATCAATAGATCGGAAGA1349290.11209946337664778No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT1341360.11144063633088533No Hit
GTTTAGTGGTAAAATCCATCGCTGTAGGCACCATCAATAGATCGGAAGAG1320950.10974496672130002No Hit
TGTACCAACACTAGTGGGCCATTTTTGGCTGTAGGCACCATCAATAGATC1314990.10924980793280768No Hit
TGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGAT1279280.10628300921853566No Hit
GTGTAGCGGTAACATCGTTGCTGTAGGCACCATCAATAGATCGGAAGAGC1276000.10601050572419761No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAATAGATC1252460.10405479467032017No Hit
ACGACCAGCGAATCCCTGGTGTTGTATGTCTGTAGGCACCATCAATAGAT1212250.1007141344546697No Hit
CGCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAATAGAT1207810.10034525777330963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)