FastQCFastQC Report
Sat 16 Sept 2023
SRR15359374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101934587
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1712527516.800259366332646No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1643728116.125322605172276No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGA33193973.256399125843321No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA31371563.0776168249938562No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA26623662.6118377268747848No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG24465522.4001195982674655No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG22509162.208196517242965No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG21994492.1577062945278818No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG8066040.7912956963272927No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG6755420.6627210840614874No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG5880370.5768768161095311No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCG5761340.565199719698673No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA5001800.4906872286636135No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT4490030.44048150212253273No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA4434260.4350103463900825No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT4056400.39794147593887835No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAG3954070.3879026850817574No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC3887610.38138281759065745No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG3704520.3634212987982185No Hit
TGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC3638080.35690339334969784No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC3144140.30844682776808624No Hit
ACTATGCCGATTAGGGATCGGACGGTGCTGTAGGCACCATCAATAGATCG3117940.3058765519891693No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC3034570.2976977774972493No Hit
TTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC2705140.26537999315188276No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAATAGATCGGAAGA2422810.23768281908082878No Hit
GTCTAGTGGTATGATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2353810.23091377218215442No Hit
CTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATCG2259950.221705906357378No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG2150510.2109696093633067No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAATAGATCGGAAG2148460.2107684999989258No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA2109790.20697489067179917No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAATAG2000720.1962748914654454No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1979440.19418727816104261No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAATAGATC1977610.1940077512650343No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1760970.1727549060457762No Hit
GTGTAGCGGTAACATCGTTGCTGTAGGCACCATCAATAGATCGGAAGAGC1683890.1651931939450542No Hit
ATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATCGG1666680.16350485630554426No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG1664390.16328020243021144No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1650570.16192443100789725Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATA1602610.15721945290267375No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1575250.15453537865415592No Hit
GTTTAGTGGTAAAATCCATCGCTGTAGGCACCATCAATAGATCGGAAGAG1517530.14887292377022138No Hit
GCTCAGTTGGTAGAGCTTTCGCCTGTAGGCACCATCAATAGATCGGAAGA1504240.14756914647625932No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1433280.14060781940481104No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1420530.13935701726049077No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA1363330.13374557548361873No Hit
AATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC1358590.1332805713923185No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGA1264970.12409625007849398No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAATA1225930.12026634296364981No Hit
CTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCA1215170.11921076405597249No Hit
CAATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1213130.11901063571288124No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1205040.11821698948954393No Hit
TAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1182970.11605187550325778No Hit
ACCAGCGGAGGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1179710.11573206256282767No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA1168540.11463626178227415No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCCTGTAGGCACCATCAATAGAT1099360.10784955650038588No Hit
ATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATAG1096310.10755034500703868No Hit
CCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACGAT1068740.10484566931143793TruSeq Adapter, Index 7 (97% over 37bp)
ACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1044320.10245001532208102No Hit
AATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG1035670.10160143190652256No Hit
TCTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC1021830.10024369844162904No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)