FastQCFastQC Report
Sat 16 Sept 2023
SRR15359384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69767966
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1143141016.384897905723665No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA936586413.424304214343872No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGA24411933.4990170130515197No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG19103082.7380875629941683No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA18935422.7140564768650415No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG18676412.6769319891022767No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA17633032.5273819792883168No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG13611221.9509268766700179No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG5609370.8040036597885053No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG5089200.7294465199114447No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCG4646020.6659245304643108No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA4485620.6429340365175616No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG3700380.5303838154031895No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAG3696340.529804753086825No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG3241590.46462440943168676No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT3127780.44831176531647776No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA2997440.42962983900089624No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC2863720.4104634496582572No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC2683690.38465934351590525No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT2632580.3773336318848682No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC2059960.295258715153026No Hit
ACTATGCCGATTAGGGATCGGACGGTGCTGTAGGCACCATCAATAGATCG1998460.28644378137668514No Hit
TTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC1948960.2793488346786547No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG1912820.2741688069278098No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1900130.2723499205924966No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1691080.24238631236576397No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAATAGATCGGAAGA1665760.238757139630529No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAATAG1533970.21986738154298494No Hit
TGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC1480450.2121962391737205No Hit
ATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATCGG1477230.2117347093077072No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAATAGATC1474350.21132191240891268No Hit
CTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATCG1438520.2061863176575909No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG1419190.20341570513894586No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1345290.19282345138168427No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1295300.18565827187795614No Hit
GATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATA1249940.17915672072194277No Hit
AATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC1200520.1720732406044344No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAATAGATCGGAAG1187280.17017552152803192No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1119450.16045329456788235No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1085280.15555563136239345No Hit
GTCTAGTGGTATGATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1080990.15494073598189748No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA1079320.15470137111349927No Hit
TAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1064000.152505520943523No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA1056720.15146206211601468No Hit
GTTTAGTGGTAAAATCCATCGCTGTAGGCACCATCAATAGATCGGAAGAG1028720.1474487589332904No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAATAG952920.13658417388862965No Hit
AATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG888230.12731201021397126No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGA881640.1263674506434658No Hit
ATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATAG879380.12604351974371736No Hit
TCGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAA873550.12520789268817153No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAATA863280.12373587041365086No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA853820.12237994726691616Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGA853500.12233408094482788No Hit
GCTCAGTTGGTAGAGCTTTCGCCTGTAGGCACCATCAATAGATCGGAAGA849300.12173208546741923No Hit
GTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGAT809770.1160661613669517No Hit
TTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATCG793620.11375134542405896No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT793310.11370691242453593No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCCTGTAGGCACCATCAATAGAT773120.11081303416527867No Hit
TGTTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA705880.10117537323647934No Hit
GTGTAGCGGTAACATCGTTGCTGTAGGCACCATCAATAGATCGGAAGAGC705640.10114097349491313No Hit
GTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCGG703240.10079697607925105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)