FastQCFastQC Report
Sat 16 Sept 2023
SRR15359386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89786458
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA2820495831.413376391348457No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT59286576.603063682498757No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA28477803.171725517894915No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG18648372.0769691126472547No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGA18101952.016111382854639No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG16261381.8111172176989094No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG15847491.7650200657208241No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA9526781.0610486494522369No Hit
ATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC5851870.6517541876972137No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG5483850.610765823950868No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT4479270.4988803545407705No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG4475140.49842037426178454No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA4111640.4579354271888084No Hit
ATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3912380.43574277091986413No Hit
ATCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCA3671310.408893510422251No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT3465430.38596354920248666No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA3214550.3580216963230691No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAG3033300.3378349104716883No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCG2757870.3071587922535044No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT2500070.27844622181220247No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA2445630.2723829466577243No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG2429920.27063323959165425No Hit
ATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATAG2021590.22515533467196133No Hit
GATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAATA1848920.20592414949702104No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAATAG1842000.2051534319351366No Hit
TGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC1779340.19817465123749511No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC1599200.17811149204705234No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC1507770.16792844194833925No Hit
GTCTAGTGGTATGATTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1449280.16141409654449226No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG1428140.15905962121815742No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAATAGATC1427730.15901395731636947No Hit
ATCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAA1317340.14671923019838917No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCCTGTAGGCACCATCAATAGAT1274710.14197129816614437No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC1233030.13732917273560338No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA1176980.13108658323507982No Hit
ATAGATGTGCATAGTTAAACCGATCATTACTGTAGGCACCATCAATAGAT1144600.12748024874753386No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1120650.12481280863089622No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1118370.1245588727868071No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAATAGATCGGAAG1093450.12178339856106139No Hit
ACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1065450.118664888195055No Hit
ATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1062440.11832964833070929Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCCGTAGGCACCATCAATA1060300.11809130503845024No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGGCTGTAGGCACCATCAAT1059700.11802447981632153No Hit
ACTATGCCGATTAGGGATCGGACGGTGCTGTAGGCACCATCAATAGATCG1054750.11747317173375967No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1033210.11507414625933902No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1033010.11505187118529613No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1018270.11341019822833417No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGGACTGTAGGCACCATCAA1005290.11196454592294976No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCCTGTAGGCACCATCAATAGATCGG995940.11092318621144404No Hit
AATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC938560.10453246746853517No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGAGGCACCATCAATAG930720.10365928456605338No Hit
ATTAAGCGGGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT930290.10361139315686113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAATA899150.10014316412838113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)