FastQCFastQC Report
Sat 16 Sept 2023
SRR15359389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66537775
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT964410614.494181688522046No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGA38218115.743821460816807No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA29083924.371038857250637No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG15993532.4036767084562114No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG15314922.3016880260874366No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA14747632.2164296897514233No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGATC8867741.3327376817153866No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA7831941.1770667113530622No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG7686831.1552580470266702No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG7524521.1308643849302145No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA7306041.0980289016276843No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG7300381.0971782570126518No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA6718081.0096640592505535No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCG6508610.9781826939659464No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT6125210.9205612901844104No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA5549650.8340600508508137No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC4536220.6817510804952526No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG3923440.5896560262196925No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG3232960.48588339480843773No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAATAGATC2860700.429936227954722No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAATAGATCGGAAGA2788750.41912282158518227No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC2756450.41426843623791143No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT2744950.4125400947056015No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT2706670.40678697176152945No Hit
ATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATCGG2650120.39828804013960495No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAG2562180.38507148758731413No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT2491600.37446397929597136No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC2284140.3432846980530984No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAATAGATCGGAAG2275820.34203428052711415No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG2267980.3408560024737827No Hit
ATTAAATCAGTTATAGTTTATTTGATACTGTAGGCACCATCAATAGATCG2218940.3334857530177407No Hit
AACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAATAGAT2215960.3330378871250203No Hit
TTATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGATC2152030.32342981111105684No Hit
AACTATGCCGATTAGGGATCGGACGGTGCTGTAGGCACCATCAATAGATC1915300.2878515249420348No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG1788770.26883525936958363No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAATA1703960.25608911629521725No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1574410.2366189732073247No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAATAG1550360.23300448504627636No Hit
GTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATCGG1523630.2289872181629157No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1465190.22020423736742023No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA1445590.21725854223409183No Hit
ACTATGCCGATTAGGGATCGGACGGTGCTGTAGGCACCATCAATAGATCG1419090.21327584218137743No Hit
TAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC1407790.21157755876267278No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT1350920.2030305341589796No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGA1266390.19032647244366077No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAATAG1263370.18987259492821934No Hit
GTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGAT1225210.1841375068523106No Hit
CAGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAATAGATC1218400.183114028084047No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCACTGTAGGCACCATCA1187540.17847606115473502No Hit
TCGCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAATAGA1117700.1679797678837322No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT1103220.16580356045870184No Hit
ACCGGCCGGCGGCTTTCAGCATCCGGGTGCTGTAGGCACCATCAATAGAT1089230.1637009954120047No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAATAGAT1056050.1587143543648702No Hit
GCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAATAGATC1037580.15593848757341225No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1030560.154883447785863No Hit
ACCGGCCGGCGGCTTTCAGCATCCGGGTGCCTGTAGGCACCATCAATAGA1024290.15394112592433395No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA998860.1501192367794084No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCTGTAGGCACCATCA976840.14680983847145473No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGA958660.14407755594472463No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCACTGTAGGCACCATCAA954560.1434613646158141No Hit
TTTAGTTGGTGGAGTGATTTGTCTGCTTCTGTAGGCACCATCAATAGATC918010.13796824435442873No Hit
AGTATCCTTTCATTCTTATGTGGGGGCTGTAGGCACCATCAATAGATCGG916180.13769321261493941No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCACCTGTAGGCACCATC889130.13362785274981015No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCTGTAGGCACCATC887790.13342646338865405No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGGCTGTAGGCACCATCAAT865860.13013059123182283No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT841770.12651009144805336No Hit
TCGACCAGCGAATCCCTGGTGTTGTATGTCTGTAGGCACCATCAATAGAT836660.12574210664543561No Hit
TCGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAA822460.12360798057945281No Hit
GTGTAGCGGTAACATCGTTGACCTGTAGGCACCATCAATAGATCGGAAGA821540.12346971325686798No Hit
ATCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCA812640.12213212720142808No Hit
AGGGGTGTATTTATTAGATTAAAAACCCTGTAGGCACCATCAATAGATCG785260.11801717144884992No Hit
TATATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAATAGAT777870.1169065241511307No Hit
GTTTAGTGGTAAAATCCATCGTTCTGTAGGCACCATCAATAGATCGGAAG748330.11246694077161432No Hit
CGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCGG746250.11215433639011824No Hit
GCTCAGTTGGTAGAGCTTTCGCCTGTAGGCACCATCAATAGATCGGAAGA720010.10821071188509084No Hit
TAGTTGGTGGAGTGATTTGTCTGCTTCTGTAGGCACCATCAATAGATCGG718670.10800932252393472No Hit
CCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG717710.10786504357862883No Hit
AGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAATAGATCG713060.10716619243730348No Hit
AGGGGTGTATTTATTAGATTAAAAACCACTGTAGGCACCATCAATAGATC678600.10198717946309446No Hit
TACAGTATAAAAATTATATATGTATTCTGTAGGCACCATCAATAGATCGG670490.10076832295639582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)