FastQCFastQC Report
Sat 16 Sept 2023
SRR15359394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78395557
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT52999056.760466029981775No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT46537675.936263709434451No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT39280105.010500786415741No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT29711923.790000497094497No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT10400181.3266287526983194No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT9496711.211383701247253No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT8834121.1268648808758384No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT8126371.0365855299682354No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT6875070.8769718926800915No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT6563520.837231120125851No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAAT5958450.7600494502513708No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT5601560.7145251866760766No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT4855480.6193565280746713No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAAT4620500.5893828906656023No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT3517420.44867593708148534No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA3339980.42604200133433584No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT2972400.3791541400745453No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT2956500.37712596391144976No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCTGTAGGCACCATC2722240.34724416844184164No Hit
TCGCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAAT2504650.31948876898725265No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA2456690.31337107535316067No Hit
TTATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAAT2302800.29374113637587906No Hit
AGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAAT2158330.27531279610654463No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAAT2149610.27420048817307335No Hit
TGATCCTTTAGTCCCTCGAAATTTGAGGCTAGAGGTCTGTAGGCACCATC2083880.2658160844497858No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1976270.25208954124785415No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT1941810.24769388397865455No Hit
GCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1931330.246357073526501No Hit
AGTTGAACCTTGGGCTCGGCCCGTCTGTAGGCACCATCAAT1829240.23333465186043645No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT1816380.2316942527750648No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT1802720.22995180709029212No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAAT1799540.229546171857673No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1771450.22596306063620417No Hit
GCTCAGTTGGTAGAGCTTTCGCTTCTGTAGGCACCATCAAT1752970.22360578419004026No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAAT1701790.21707735299335904No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA1619320.20655762417760487No Hit
GCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAAT1599110.2039796719602362No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCCTGTAGGCACCAT1537720.19614887103869932No Hit
TGCTTTGATACTTATGGTACCAACATTTACTGTAGGCACCATCAAT1521260.194049262256023No Hit
AGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1467180.18715091213651303No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT1456780.18582430634429958No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGGCTGTAGGCACCATCAAT1455070.18560618173807986No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1422350.18143247582257754No Hit
TGCTTTGATACTTATGGTACCAACATTTCTGTAGGCACCATCAAT1256410.16026545994181787No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGAACTGTAGGCACCATCAAT1233490.15734182486897824No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAAT1219820.15559810360171303No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCACCTGTAGGCACCATCA1208840.15419751402493384No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT1202790.15342578661696352No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT1192920.15216678669685324No Hit
TCGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAA1162280.14825840193979362No Hit
GCTCAGTTGGTAGAGCTTTCGCCTGTAGGCACCATCAAT1133550.14459365343880393No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAAT1133400.14457451970141624No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1127450.14381554811837105No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT1093780.13952066186607998No Hit
TGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA1054990.1345726773776223No Hit
TGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT1015750.12956729167700154No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCTGTAGGCACCATCA1015290.12950861488234594No Hit
GTTTAGTGGTAAAATCCATCGTTCTGTAGGCACCATCAAT1011210.12898817722540068No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1008930.12869734441710773No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1004990.12819476491505763No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT999510.12749574570916053No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN977480.12468563747815453No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT971930.12397768919480984No Hit
TTGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT894070.11404600390810414No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT886260.11304977398145152No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCTCTGTAGGCACCATCAAT878810.1120994650245294No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCACTGTAGGCACCATCAA861630.10990801430239217No Hit
CAGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT847480.10810306507548637No Hit
ACTATGCCGATTAGGGATCGGACGGTGTCTGTAGGCACCATCAAT838950.10701499320937283No Hit
TAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT836120.10665400336399165No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTTCTGTAGGCACCATCAAT834630.10646394157260723No Hit
GTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT819390.10451995385401752No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT791700.10098786593224919No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)