FastQCFastQC Report
Sat 16 Sept 2023
SRR15359397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89926470
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT65558167.290196090205699No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT52793695.87076196808348No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT51018035.673305090258742No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT44384814.93567800448522No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT11741481.305675625875229No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT11013401.2247117005704773No Hit
AGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAAT7836380.871420839714936No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA5496050.6111715493780641No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT5008950.5570050731447592No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT4958310.5513738057326169No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT4290680.47713203909816543No Hit
TTTTGACCCGTTCGGCACCTTACGATAAATCCTGTAGGCACCATCAAT4242920.4718210333397942No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT3665610.40762302801388733No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA3475020.38642904586380405No Hit
AATTCTCACGCCAGGTCGTACTCATAACCGCCTGTAGGCACCATCAAT3398750.3779476721370248No Hit
AAAATCAGTTATAGTTTATTTGATAGTACCTGTAGGCACCATCAAT3385460.3764697980472268No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT3183820.35404703420472305No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT3107030.34550783545712405No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGCTGTAGGCACCATCAAT2986040.33205350993984306No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT2750360.30584543127290553No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT2619340.2912757500655813No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCCTGTAGGCACCATCAAT2520070.2802367311871577No Hit
TAGCCTTCATCCATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAAT2498000.2777825038612102No Hit
TTCATCCATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAAT2486570.27651146542280597No Hit
GCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT2311890.25708670650588195No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAAT2230110.24799260996233924No Hit
TTTTTGACCCGTTCGGCACCTTACGATAAATCCTGTAGGCACCATCAAT2171700.24149730329679348No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT2046410.22756480933811812No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAAT1993280.22165664903781948No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1686330.18752320645967754No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT1679290.18674034463934813No Hit
ATTAAATCAGTTATAGTTTATTTGATAGTACCTGTAGGCACCATCAAT1516580.16864667322091037No Hit
AGGTGAGAGCTTCGGCGCATCATCGACCGATCCTGCTGTAGGCACCATCA1448670.16109494790577236No Hit
TGCTTTGATACTTATGGTACCAACATTTACTGTAGGCACCATCAAT1353410.15050184889943974No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1312630.145967032843611No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT1284440.14283224950339982No Hit
TTTCTGGAGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1267890.14099185701384698No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT1213830.13498027888785138No Hit
TATTCTCACGCCAGGTCGTACTCATAACCGCCTGTAGGCACCATCAAT1192890.13265170978022378No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1190000.1323303361068215No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCATGCCCTCTGTAGGCACCA1128390.1254791831593078No Hit
AAAATCAGTTATAGTTTATTTGATAGTACCCTGTAGGCACCATCAAT1119370.12447614145201073No Hit
AAATCAGTTATAGTTTATTTGATAGTACCTGTAGGCACCATCAAT1091030.12132467781733232No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAAT1090730.12129131722839782No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAAT1074780.11951764591671395No Hit
TTTGACCCGTTCGGCACCTTACGATAAATCCTGTAGGCACCATCAAT1058130.11766613323084961No Hit
TAGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAAT1052180.11700448155031548No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT1038810.11551771130346826No Hit
TTTCTGGCGAGCATGCCTGTTCGAGAGTCATTCTGTAGGCACCATCAAT997880.1109662149531723No Hit
ATTCTGGAGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT993640.1104947186295648No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT967660.10760569162783772No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT965100.10732101460226338No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAAT955630.10626793201156455No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCATGCCCCTGTAGGCACCAT950550.10570302603894047No Hit
TGATCCTTTAGTCCCTCGAAATTTGAGGCTAGAGGTCTGTAGGCACCATC947560.1053705321692267No Hit
AATTCTCACGCCAGGTCGTACTCATAACCGCAGCCTGTAGGCACCATCAA910640.10126495569102179No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT910080.1012026825916774No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)