FastQCFastQC Report
Sat 16 Sept 2023
SRR15359407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77715534
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT790958410.177610051550312No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT65586958.439361685399987No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT49028816.3087528935978225No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT47320066.0888805061803986No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT29508623.7970040841513097No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT11113321.4299998247454622No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT9804371.2615714639495368No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT7460550.9599818229390279No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT7118510.9159700298784539No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT5055290.6504864265617734No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT4710750.6061529474918104No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT4285390.5514200031103177No Hit
GTTTAGTGGTAAAATCCATCGTCTGTAGGCACCATCAAT4130990.5315526751704492No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT3743000.4816282932573043No Hit
TGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA3127150.4023841617044026No Hit
TGCTTTGATACTTATGGTACCAACATTTACTGTAGGCACCATCAAT2973080.38255929631777347No Hit
GTCTAGTGGTATGATTCTCGCTTCTGTAGGCACCATCAAT2958340.38066263560641556No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAAT2797880.360015540779788No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA2743060.35296160996590464No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA2480950.31923476199751777No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT2405300.3095005433534047No Hit
AGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT2360500.30373593006515276No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT2250870.2896293551814236No Hit
GTGTAGTTGGTTATCACGTCAGTCTGTAGGCACCATCAAT2206010.28385702142894625No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAAT1984040.2552951640273102No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT1835030.23612139112368447No Hit
GCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1705780.21949022443826996No Hit
TGATCCTTTAGTCCCTCGAAATTTGAGGCTAGAGGTCTGTAGGCACCATC1672190.2151680512161185No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT1606480.20671285614533638No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1528640.19669684055699854No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT1519780.1955567853397237No Hit
GTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT1504240.1935571851053613No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1475240.18982562739644818No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAAT1398400.17993828621186597No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1396440.17968608438050493No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT1371710.17650396637562835No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAAT1357840.17471925239553782No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCTGTAGGCACCATC1348320.17349427207178425No Hit
TGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT1320400.16990168271892722No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCCTGTAGGCACCAT1169590.1504962958885414No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGCTGTAGGCACCATCAAT1165080.14991597432760354No Hit
TTTCTGGAGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1102970.14192400711034167No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAAT1066290.13720422998058535No Hit
TTGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT1026290.13205725383036035No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT986930.12699262929853894No Hit
TGCTTTGATACTTATGGTACCAACATTTCTGTAGGCACCATCAAT934010.12018317985179128No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGTCTGTAGGCACCATCAAT928440.11946646342287244No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT895190.11518803949799791No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAAT882900.11360663107584129No Hit
TTTCTGGCGAGCATGCCTGTTCGAGAGTCATTCTGTAGGCACCATCAAT877900.11296325905706317No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT871770.11217448496204119No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT862370.1109649455667383No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAAT841240.10824605541538196No Hit
GGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT838360.10787547313256575No Hit
TTGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT829320.1067122565226149No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT825700.10624645518101954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)