FastQCFastQC Report
Sat 16 Sept 2023
SRR15359409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15359409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76093154
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT65458838.602459821812616No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT53925997.08683858734519No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT53434797.022286131023035No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT46646176.130140170034219No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT20882522.744336238185107No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT17078342.2443990165002226No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT15641072.055516058645696No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT8760571.151295424027239No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAAT8538411.1220996306711113No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT6891820.9057082848740899No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT5614480.7378429865057242No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT4418890.5807210987732221No Hit
TGCTTTGATACTTATGGTACCAACATTTACTGTAGGCACCATCAAT3914520.5144378691412896No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAAT3602580.4734433796764424No Hit
CGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT3168850.41644350817683284No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT3088090.4058302012294036No Hit
TCTTCCGTAGTATAGTGGTCAGTATGTGAGCCTGTAGGCACCATCAAT2884780.37911163466821207No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA2807430.36894646264761216No Hit
TAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT2488050.32697422425150097No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT2240930.29449824093242344No Hit
TTGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAAT2160880.28397824067063904No Hit
TCGGGTATGGTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAAT2088650.2744859281296186No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCCTGTAGGCACCATCAA2082040.27361725602805215No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGACTGTAGGCACCATCAAT2052490.26973385805508865No Hit
TGTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAAT2013230.264574392592532No Hit
GTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT1987050.26113387283171363No Hit
TGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT1955360.25696924062314463No Hit
TGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGACTGTAGGCACCATCAA1950830.2563739176851573No Hit
TTGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT1900620.249775426577797No Hit
TGTTACGATCTGCTGAGGGTAAGCCCTCCTGTAGGCACCATCAAT1788610.23505531128332519No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCCTGTAGGCACCATCAAT1750230.23001149354382128No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCCTGTAGGCACCATCAAT1711920.22497687505501482No Hit
TGATCCTTTAGTCCCTCGAAATTTGAGGCTAGAGGTCTGTAGGCACCATC1480570.19457335150018884No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT1438750.1890774562978425No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGAACTGTAGGCACCATCAAT1431680.1881483319774076No Hit
TCGATGGGCCCCGCGTTCGATTCGCGGCTGGCGCTGTAGGCACCATCAAT1325010.1741299880932784No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1200320.15774349424391057No Hit
GCATAAGCATGGCAGGAAAGCTAAGTTAGTCACTGTAGGCACCATCAAT1192850.1567618027766335No Hit
TGCTTTGATACTTATGGTACCAACATTTCTGTAGGCACCATCAAT1185910.15584976277892226No Hit
TCGCGTATTGTAATTTTAACACGCGCTGGACTGTAGGCACCATCAAT1182320.15537797263601402No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAAT1149700.15109112181103704No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAAT1095520.14397090177126842No Hit
AGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT1033980.1358834462296043No Hit
CGCTCGAGACCCGGGTTCAATTCCCGGCGGGAGAGCCTGTAGGCACCATC1016330.13356392087519464No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAAT1012640.13307898894557585No Hit
TAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAAT998810.13126147984350867No Hit
TCAACAGCCCCGGGTTCGACTCCCGGTATCCGCTGTAGGCACCATCAAT939580.12347759957485796No Hit
TTTCTGGAGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT922020.12116990182848775No Hit
TCGAGAGGACCCAGGTTCGATTCCTGGCAATCCCCTGTAGGCACCATCAA918810.12074805047507953No Hit
TTATATATCAAATCATCTTTATGCTATTCTGTAGGCACCATCAAT917750.12060874753594786No Hit
TTTCTGGCGAGCATGCCTGTTCGAGAGTCATTCTGTAGGCACCATCAAT911880.11983732465603936No Hit
GCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT894990.11761767688062977No Hit
CTAGAGGTTATCAACATATGAACTCTTAGCTGTAGGCACCATCAAT889770.11693167561433976No Hit
CTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAAT864760.11364491475803461No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCCTGTAGGCACCATCAAT825220.10844865229268852No Hit
GTTACGATCTGCTGAGGGTAAGCCCTCCTGTAGGCACCATCAAT821830.10800314572320133No Hit
CAGTATCCTTTCATTCTTATGTGGGGGACTGTAGGCACCATCAAT805540.10586234866805494No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGTCTGTAGGCACCATCAAT783370.10294881455432904No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCCGTAGGCACCATCAAT772290.10149270458680158No Hit
CGAGAGGACCCAGGTTCGATTCCTGGCACTGTAGGCACCATCAAT770130.101208841993854No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT767100.10081064585652476No Hit
GTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAAT765370.10058329294643246No Hit
AGGGGTGTATTTATTAGATTAAAAACCACTGTAGGCACCATCAAT761580.10008521923010315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)