FastQCFastQC Report
Wed 28 Jun 2023
SRR1536296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1536296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79737969
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG10977551.3767029857507407No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6585060.8258374376202132No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG5735360.7192759073158737No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA4832180.6060074090926494No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG4498310.5641365156917905No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA4474900.5612006495926678No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG4418980.5541876793977534No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC4350010.5455380986691547No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA4331480.5432142371220918No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA3299940.4138480126073941No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG2959540.37115818688584856No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT2949020.3698388655973919No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG2898020.3634429163852919No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT2803010.3515276392354563No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC2579360.3234795207788651No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA2527210.3169393491825707No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA2504230.3140574097140598No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2175280.27280353729601514No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA2059650.25830229009218936No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG2050050.25709834671108817No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT2033740.25505289707090484No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1946750.24414341428736414No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCG1920130.24080497962018574No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCTGTAGGCACCATCAATAGATC1909160.2394292234857399No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1871710.2347325901917567No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1868450.23432375108525774No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1853600.2324614011676169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1800140.22575694146410977No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCCTGTAGGCACCATCAATAGAT1591530.1995950009712437No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1581280.19830954059038047No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1573980.19739404197766813No Hit
ATGCTTAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1542490.19344485686611856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1456960.18271847380512035No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1429160.17923205443068158No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1422580.1784068515715518No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1414090.17734211414389048No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGAT1385240.17372401346214375No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA1336100.16756132828013215No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1330090.16680760955925528No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA1295810.16250852840257318No Hit
CCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAG1279270.16043423428555098No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG1278450.1603313974550819No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCG1142090.14323038501269075No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC1131590.14191357193961135No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT1094560.13726961116855133No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATTG1066690.133774413040292No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1062070.13319501528813707No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCGG1057330.13260056824371838No Hit
CCCCCCGCCTCCCCTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1051180.13182929201520044No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAG1043130.13081973532583957No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAG1009210.12656580204594878No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG999700.12537314563404542No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT988800.12400616825342015No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG966900.1212596724152831No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGAA954870.11975098086584073No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA950160.11916029614448796No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA940530.11795259044032085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA939360.11780585984074914No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT914630.114704451526725No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGA908580.11394571637509353No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT881320.11052701881584168No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG872750.10945224852667115No Hit
ATCCGAGTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG855730.10731775724059386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA831050.10422261946501296No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA828730.10393166648124685No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGTACCATCAATAGATCG817480.10252079533151892No Hit
TTTGGCAATTTTTGACAGTCTCTACGGAGACTGCTGTAGGCACCATCAAT797700.10004017032337505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)