FastQCFastQC Report
Wed 28 Jun 2023
SRR1536297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1536297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49732175
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG6016471.2097741552626644No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG3975000.7992813505542438No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG3813940.766895877769271No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3624760.7288561177949687No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA3149830.6333585852619557No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG3138180.6310160374043565No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC2902830.5836925491394656No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA2783240.5596457424192688No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA2767720.5565250263033942No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG2551680.5130843362471076No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA2531760.509078881026217No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA2128840.42806090825506826No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT2126310.427552183269684No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG1920100.3860880808048311No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1814580.36487042844999235No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA1809980.363945473931112No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG1716770.34520308029962493No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT1608960.32352496145603926No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1520610.3057598023814563No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC1467560.2950926638539336No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT1428300.2871983781123588No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1411140.2837478956027964No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1336880.2688159124349579No Hit
CCCCCCGCCTCCCCTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1168740.23500681399918666No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1148420.2309209279505672No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1101930.2215728549977957No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1096230.22042671570266129No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1090790.2193328564455506No Hit
CCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAG1086250.21841996655082951No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCG1069820.215116270301872No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA1057270.21259275308188313No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1045100.21014564514823653No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1030390.20718780145851254No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCTGTAGGCACCATCAATAGATC1024030.20590895129762574No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT996120.20029689029285366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC987180.1984992612931166No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA894800.17992376162916662No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGAT894110.17978501845133457No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCCTGTAGGCACCATCAATAGAT869110.17475809171828902No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG856830.17228886530701704No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG846700.17025195459478698No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA841430.16919227843946097No Hit
ATGCTTAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG831390.16717346466346988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC817800.16444082729138632No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGAA812860.1634475065689365No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA793440.15954258988270673No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG765720.1539687335211058No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG757270.1522696322853364No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG752370.15128435464565945No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT718680.14451006818020729No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAG666260.13396960820635737No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATAGATCGGA660020.1327148872937892No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC657380.13218404383077959No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGA634830.12764975591757247No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG625520.12577772840218632No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG624300.1255324143776137No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCG622900.12525090648056314No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT602810.12121126815788773No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATTG587940.11822125213707221No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCGG578820.1163874292648572No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA577150.11605163055908978No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT574880.11559518561172921No Hit
TCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATCG564310.11346980098899757No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAG557580.11211655231246172No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA541880.10895964232410908No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG533600.1072947241901244No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA531870.10694686086019764No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA525890.10574441998565315Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC525890.10574441998565315No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT515200.10359490611460287No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT514580.10347023833162333No Hit
CCCCCCGCCTCCCCTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA507820.10211095734300782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)