FastQCFastQC Report
Wed 28 Jun 2023
SRR1536298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1536298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181384849
Total Bases9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG19490401.074532967193969No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG14083310.7764325453665647No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG13305030.7335248822243141No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG12872560.7096822072498459No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA11013560.6071929414567586No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC10957850.6041215713667463No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG10369400.5716795011914143No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA9840190.5425034149351691No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA9699090.5347243749118208No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG8516950.4695513460443435No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT7747490.42712994181779756No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA7539930.41568686919379905No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG7244450.39939664420372845No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA7186610.39620784423951527No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA6433420.3546834278313951No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA6338360.3494426372954667No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT5740590.3164867425062608No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG5720760.31539348691687036No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA5428870.29930118363965447No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT5213620.28743415057781374No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA4986790.2749286959463742No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC4915140.2709785313987278No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC4326020.23849952318784903No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA4157190.22919168954403682No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4097780.2259163332875724No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4018060.22152125837147513No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA3785750.20871368368810117No Hit
CCCCCCGCCTCCCCTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC3774400.20808794233966038No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3772950.20800800181496967No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCTGTAGGCACCATCAATAGATC3744340.20643069256572802No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA3682350.20301309730671055No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3673980.20255164751935814No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG3632680.20027472085058215No Hit
CCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAG3605570.1987801086958481No Hit
ATCCGAGTCACGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3502240.19308338151220117Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCG3457220.19060136604904634No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGAT3377020.1861798280627066No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA3370420.18581596084687316No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG3183850.1755300962320177No Hit
TAAGTGGGAGGCCCCCGGCGCCCGGCCCCCTGTAGGCACCATCAATAGAT3164540.17446550896872318No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG3015120.1662277757278393No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG3011630.1660353671546183No Hit
ACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGATCGGA2975580.16404788031661896No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG2961230.1632567447791629No Hit
ATGCTTAGATGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG2933240.16171361699565107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC2892960.15949292435114026No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGAA2851690.15721765162425447No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2718180.14985705889911458No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA2645950.14587491814159187No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2624950.14471715881848546No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT2578590.14216126728423717No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAG2511460.13846029664804033No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC2376840.1310385080729648No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG2345940.1293349479261082No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGA2329370.1284214206887809No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG2279770.1256869034303962No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATAGATCGGA2262040.12470942377331637No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATA2185010.12046265231336936No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAAT2114520.11657644018547547No Hit
TCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATCG2076250.11446656164760485No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT2065890.11389540038153903No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2055180.11330494312675476No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCG2024390.1116074474334954No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCCTGTAGGCACCATCAATAG1993630.10991160568212618No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1957490.10791915701845638No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCGG1924790.10611636035819066No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1921320.10592505441289642No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATTG1896070.1045329866553518No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1895550.10450431832925583No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1885940.10397450561044379No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAGAT1820510.10036725834802222No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1819980.10033803870796287Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1815360.10008333165687947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)