FastQCFastQC Report
Wed 28 Jun 2023
SRR1536299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1536299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80976587
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG13534911.671459677597921No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG11256081.3900412967516154No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA8105321.0009461129795456No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC7689350.9495769437652393No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG7637720.9432010267362836No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA5735030.7082331094048209No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG5451830.6732600374970114No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG5111690.6312553034619748No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT4630360.5718146653921089No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA4481010.5533710626751903No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT4275880.5280390491142829No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA3112490.3843691263500646No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2878230.3554397766850806No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC2811830.347239875644549No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2724350.33643675300861964No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC2660010.32849124648832134No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA2525460.3118753325575453No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA2521030.311328260846558No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC2420950.2989691328926965No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG2378630.2937429309042131No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCG2370380.2927241178984242No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGAT2351720.2904197481180579No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2315280.2859196819446095No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2266420.2798858391006279No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG2248530.2776765585341353No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA2224570.2747176785803531No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2047830.25289161668421517No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2034690.25126892542408585No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT1980160.2445348801870348No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1951040.24093877900781369No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG1795320.22170852915794045No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1787070.22068971615215147No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG1758880.21720846298449203No Hit
CCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1736440.21443729160874614No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1647030.20339582847570498No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA1500680.1853227032154368No Hit
CCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAG1452460.1793678955622074No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCGG1447410.178744258510179No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCG1413680.17457885697257158No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT1402780.17323278888007468No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC1397920.17263261540030084No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1370030.16918840997830645No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1342170.16574790933087857No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1326080.16376091523837624No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT1308110.1615417552730396No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG1272910.15719481978167343No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1271140.15697623808224964No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGA1231940.15213533264868276No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA1225890.15138820311110418No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1212070.1496815369608008No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAG1190640.14703509299546053No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATTG1183030.1460953151804237No Hit
CCCCCCGCCTCCCCTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1179630.1456754407295531No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1171050.1446158752035326Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1163630.1436995609607503No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG1146890.14163229675264036No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1091810.13483033064853672No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG1038980.12830622263692096No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1002350.12378269289121804No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG972140.12005198490274727No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG949690.11727957860214583No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT946700.1169103360703508No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA946180.11684611997786472No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC935690.11555068380444337No Hit
CCGACGTACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGG910060.1123855713997924No Hit
TCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCGG884740.10925874166566195No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGTACCATCAATAGATCG881400.10884627676392437No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG868000.10719147745755203No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCATCATCAATAGATCG864660.10677901255581443No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCGG859990.10620230264829511No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGTACCATCAATAGATCGG844350.10427088017429038No Hit
ACCGCCCGTCCCCGCCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCA828320.10229129563092108No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT822240.101540461318776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)