FastQCFastQC Report
Wed 28 Jun 2023
SRR1536301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1536301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124212409
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG16242951.3076753064180568No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG16026971.2902873496318714No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATC13230961.065188261504533No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA10958880.8822693391285891No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA9485650.7636636368593415No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCG9260230.7455156915924559No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG9248990.7446107900539953No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA7714990.6211126619402414No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT7662530.6168892513790631No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG6731100.5419023794957556No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAAT6361140.512117915690694No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA5338730.42980649381013136No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5183410.41730210707047793No Hit
CCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGA5151180.4147073582640201No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC4947670.39832332693909833No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA4721710.38013190775488465No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCAA4523570.36418020038561527No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG4384810.3530090137773594No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4200280.3381530101392688No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG4079590.3284365896164207No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCGG4049070.32597950821483546No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGAT3837320.3089320971143873No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG3743030.3013410681053613No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG3702200.2980539569118251No Hit
CCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA3523830.283693877960293No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGAT3517140.28315528442894944No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAG3418410.27520680321078067No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3175220.2556282440347808No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA3021510.24325347397456884No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC2961030.23838439523381277No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC2915240.23469796805889176No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA2905810.23393878465073487No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCG2824360.22738146878706783No Hit
CCCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAG2761160.22229341031458458No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATCG2730070.2197904397780418No Hit
CCCCCCGCCTCCCCTCCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2711680.21830991137125438No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC2665890.2146234841963334No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA2640200.21255525283307244No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGA2563460.20637712613721226No Hit
TGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG2555560.20574111882815188No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2485660.200113661751782No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGAT2468950.19876838553223777No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGA2443140.19669049329845945No Hit
TCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATAGATCG2386390.19212170661628503No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA2274090.18308074195710994No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATAGATCG2258130.1817958461782993No Hit
CCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2155400.17352533594288475No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTCTGTAGGCACCATCAATAGA2120810.17074059001625191No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT2076440.16716848314245317No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGATC2050170.16505355757169157No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAG2007900.16165051593194688No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATCA1876640.15108313373102683No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1802850.14514250343538543No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAG1784770.14368693227743454No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1784090.14363218734450275No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATTAATAGATCGG1772670.14271279450026608No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACTATCAATAGATCG1700070.1368679678372553No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1684670.13562815612085907No Hit
TCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATCGG1667110.13421444873515012No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG1656130.13333047908281048No Hit
CCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATAGATC1636970.13178796009020322No Hit
TGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG1611690.12975273670120996No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1603360.12908211127279562No Hit
CCGACGTACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGG1524930.12276792731714913No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1520920.12244509322736025Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG1519790.12235412002998831No Hit
ACCGCCCGTCCCCGCCCCTCTGTAGGCACCATCAATAGATCGGAAGAGCA1513310.12183243302205016No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG1433170.11538058166153109No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATTG1411720.11365370105655064No Hit
ACCGCCCGTCCCCGCCCCTTCTGTAGGCACCATCAATAGATCGGAAGAGC1405500.11315294593473345No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1374980.11069586453314821No Hit
ACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1349220.10862199766208543No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAG1326490.10679206777158633No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1322290.10645393730347827No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1318090.1061158068353702No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATAGAT1312650.10567784737191596No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCCTGTAGGCACCATCAATAGAT1303580.10494764657531115No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC1300630.10471015017509241No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCACCATCAATAGA1288210.10371025007654427No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAG1279590.10301627754437964No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1257220.10121533026543265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)