FastQCFastQC Report
Sat 23 Sept 2023
SRR15404514_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15404514_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131897004
Total Bases19.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA60740354.605134927856284RNA PCR Primer, Index 29 (100% over 39bp)
GGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA53765164.07629880660519RNA PCR Primer, Index 7 (100% over 35bp)
AGCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA50172293.8038991393618007RNA PCR Primer, Index 29 (100% over 40bp)
TTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA36122702.738705118730369RNA PCR Primer, Index 28 (100% over 36bp)
AGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT29870382.2646746396150137RNA PCR Primer, Index 29 (100% over 38bp)
GACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA27660032.097093122752053RNA PCR Primer, Index 29 (100% over 39bp)
TGGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT16560071.2555304137158414RNA PCR Primer, Index 29 (100% over 41bp)
AGCCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT14005201.061828515831944RNA PCR Primer, Index 29 (100% over 41bp)
CTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC13841201.0493945715400783RNA PCR Primer, Index 29 (100% over 37bp)
TAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC12483130.9464301402934066RNA PCR Primer, Index 29 (100% over 37bp)
GACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT11857690.8990113225013056RNA PCR Primer, Index 29 (100% over 38bp)
GGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA11221670.8507903636689124RNA PCR Primer, Index 29 (100% over 40bp)
CGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT10394740.7880952322465187RNA PCR Primer, Index 29 (100% over 41bp)
AAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA10243840.7766544871633324RNA PCR Primer, Index 29 (100% over 40bp)
TTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA9262470.7022502194212084RNA PCR Primer, Index 29 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC6983100.5294358316129758No Hit
AGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC6977600.5290188395787974RNA PCR Primer, Index 29 (100% over 44bp)
TCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA6617690.501731639029496RNA PCR Primer, Index 29 (100% over 40bp)
TCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA6468210.4903985537078613RNA PCR Primer, Index 28 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA6227380.47213960978219033No Hit
TTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA5892180.4467258407173525RNA PCR Primer, Index 7 (100% over 35bp)
TGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC5849070.44345738133672846RNA PCR Primer, Index 29 (100% over 37bp)
TTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC5405230.4098068823458643RNA PCR Primer, Index 29 (100% over 37bp)
CTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT5363500.4066430500574524RNA PCR Primer, Index 29 (100% over 41bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC5339980.4048598404858385RNA PCR Primer, Index 29 (100% over 44bp)
GACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC5233410.3967800511981303RNA PCR Primer, Index 29 (100% over 42bp)
CCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT5185540.39315070416610826RNA PCR Primer, Index 29 (100% over 43bp)
ACTCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT4997380.3788850275931969RNA PCR Primer, Index 29 (100% over 41bp)
CGGGGAGAGTTCTCTTTTCTTTGTGATGGAATTCTCGGGTGCCAAGGAAC4781490.36251695300069137RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA4714550.3574417808610725No Hit
TGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC4459630.33811457916056986RNA PCR Primer, Index 29 (100% over 37bp)
AGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT4452650.3375853783608307RNA PCR Primer, Index 29 (100% over 41bp)
TCTCGTCTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC4343400.32930240022737745RNA PCR Primer, Index 29 (100% over 37bp)
GAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT4308680.32667004324070925RNA PCR Primer, Index 29 (100% over 38bp)
GCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA4258080.32283371652626774RNA PCR Primer, Index 28 (100% over 36bp)
CGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT4134560.3134688336059551RNA PCR Primer, Index 29 (100% over 41bp)
CAGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA3960580.3002782383138892RNA PCR Primer, Index 29 (100% over 40bp)
CGGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT3939790.2987020084246948RNA PCR Primer, Index 29 (100% over 43bp)
TAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT3902650.2958861749429881RNA PCR Primer, Index 29 (100% over 43bp)
AGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA3856670.2924001215372565RNA PCR Primer, Index 29 (100% over 39bp)
TAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA3782110.28674722588846674RNA PCR Primer, Index 29 (100% over 40bp)
CGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3755950.2847638601404472RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC3547960.26899473774248883RNA PCR Primer, Index 29 (100% over 44bp)
CCGGATCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT3499380.26531156082969104RNA PCR Primer, Index 29 (100% over 41bp)
CGCTGGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA3476440.2635723249634995RNA PCR Primer, Index 29 (100% over 39bp)
TCTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC3185880.24154301488152072RNA PCR Primer, Index 29 (100% over 37bp)
ACAGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT3054040.23154733673859643RNA PCR Primer, Index 29 (100% over 41bp)
CATTCGTGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT3044510.23082480326846547RNA PCR Primer, Index 29 (100% over 38bp)
CGGGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT3022240.22913636461371023RNA PCR Primer, Index 29 (100% over 41bp)
TTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3005640.22787780683782627RNA PCR Primer, Index 1 (100% over 32bp)
ACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2778230.21063632347555067RNA PCR Primer, Index 29 (100% over 40bp)
AAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT2755450.2089092182867171RNA PCR Primer, Index 29 (100% over 43bp)
AGCCGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2664610.20202202621675924RNA PCR Primer, Index 29 (100% over 42bp)
CGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCG2617880.19847911026091236RNA PCR Primer, Index 29 (100% over 45bp)
GACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2613590.1981538564742532RNA PCR Primer, Index 29 (100% over 40bp)
CTTCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2585970.19605979829534262RNA PCR Primer, Index 29 (100% over 40bp)
ATTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2556840.1938512568488667RNA PCR Primer, Index 29 (100% over 42bp)
CGCGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2529950.19181254488540164RNA PCR Primer, Index 29 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2528980.19173900265391927No Hit
TTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT2419130.1834105344803738RNA PCR Primer, Index 29 (100% over 38bp)
TGGTGGTGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2415610.1831436595784996RNA PCR Primer, Index 29 (100% over 40bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2398840.18187221295792283No Hit
TGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC2378120.18030129024007246RNA PCR Primer, Index 29 (100% over 37bp)
CTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT2361260.17902302011348187RNA PCR Primer, Index 29 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2356180.17863787110736798No Hit
CGTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2336490.17714503962500922RNA PCR Primer, Index 29 (100% over 42bp)
CCTAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT2335150.17704344520213666RNA PCR Primer, Index 29 (100% over 41bp)
TCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2324580.17624206232917922RNA PCR Primer, Index 29 (100% over 42bp)
CGCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2318290.17576517507554607RNA PCR Primer, Index 29 (100% over 42bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2293850.1739122141091241RNA PCR Primer, Index 29 (100% over 42bp)
TCGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2277730.1726900483653139RNA PCR Primer, Index 29 (100% over 40bp)
ACCGATTGGATGGGACGCTCATCAGACCTGGAATTCTCGGGTGCCAAGGA2273320.17235569657063626RNA PCR Primer, Index 1 (100% over 22bp)
TCGGCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT2257030.17112064198213328RNA PCR Primer, Index 29 (100% over 41bp)
TTTTGATCCTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA2256100.17105013241999037RNA PCR Primer, Index 28 (100% over 36bp)
CGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC2128590.16138274073306472RNA PCR Primer, Index 29 (100% over 44bp)
AGGGGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC2125420.16114240168791097RNA PCR Primer, Index 29 (100% over 42bp)
CTGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA2124010.16103550009369433RNA PCR Primer, Index 28 (100% over 36bp)
CAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT2122530.16092329132813357RNA PCR Primer, Index 29 (100% over 41bp)
TTGGATTGTTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT2113490.16023790805741123RNA PCR Primer, Index 29 (100% over 38bp)
ACAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA2101190.15930536223552128RNA PCR Primer, Index 29 (100% over 40bp)
CAGCCTCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA2092310.15863210964215685RNA PCR Primer, Index 7 (100% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC1931930.14647262192551394No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTAT1931550.1464438115667889RNA PCR Primer, Index 29 (100% over 48bp)
ACCGATTGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC1927660.1461488844735245RNA PCR Primer, Index 29 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1915960.14526182869172677No Hit
CGGGTTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1904900.14442329561936068RNA PCR Primer, Index 29 (100% over 41bp)
GACTGAACGCCTCTAAGTCATGGAATTCTCGGGTGCCAAGGAACTCCAGT1901460.14416248605616547RNA PCR Primer, Index 1 (100% over 30bp)
CGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1897720.14387893147292413RNA PCR Primer, Index 29 (100% over 42bp)
CGGTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1892420.1434771027854431RNA PCR Primer, Index 29 (100% over 42bp)
CGACCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1884530.14287890875823078RNA PCR Primer, Index 29 (100% over 41bp)
TGCGGGTCATAAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1827800.13857782546751404RNA PCR Primer, Index 7 (100% over 35bp)
GGGTCGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1766040.13389538400735776RNA PCR Primer, Index 1 (100% over 29bp)
AGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1741570.13204014853893117RNA PCR Primer, Index 29 (100% over 41bp)
TGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC1718910.13032214135811607RNA PCR Primer, Index 29 (100% over 44bp)
TGGGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1656940.12562377838392752RNA PCR Primer, Index 29 (100% over 41bp)
TAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT1656620.12559951702921168RNA PCR Primer, Index 29 (100% over 38bp)
TCCCGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1644440.12467606921534019RNA PCR Primer, Index 29 (100% over 41bp)
GGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCG1635430.12399296044662243RNA PCR Primer, Index 29 (100% over 45bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGC1628760.12348726283426421RNA PCR Primer, Index 29 (100% over 50bp)
AGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1578460.11967368113986879RNA PCR Primer, Index 29 (100% over 41bp)
CCGGTAGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT1576840.11955085803161988RNA PCR Primer, Index 29 (100% over 38bp)
CCCTGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1551040.11759478630765563RNA PCR Primer, Index 29 (100% over 41bp)
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC1551000.11759175363831614RNA PCR Primer, Index 29 (100% over 44bp)
AGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1502310.11390023688483478RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCT1496100.1134294149698806RNA PCR Primer, Index 29 (100% over 43bp)
CTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA1490260.11298664524631659RNA PCR Primer, Index 29 (100% over 40bp)
CTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA1482550.11240209823113192RNA PCR Primer, Index 29 (100% over 40bp)
ACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA1462460.11087894005537838RNA PCR Primer, Index 29 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1456670.1104399611684887No Hit
GGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1449180.10987209383467118RNA PCR Primer, Index 29 (100% over 42bp)
CTCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA1446780.1096901336743024RNA PCR Primer, Index 29 (100% over 39bp)
GGCATGTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT1424190.1079774336648314RNA PCR Primer, Index 29 (100% over 38bp)
ACTACCGATTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAC1422920.10788114641330292RNA PCR Primer, Index 29 (100% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1418870.10757408864268063No Hit
CAGGCGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA1411320.10700167230485386RNA PCR Primer, Index 29 (100% over 39bp)
CCGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA1409910.10689477071063722RNA PCR Primer, Index 29 (100% over 40bp)
GGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1391440.10549443564313257RNA PCR Primer, Index 1 (100% over 32bp)
AGTGGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1387360.10518510337050567RNA PCR Primer, Index 29 (100% over 42bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTC1382410.10480981053974509RNA PCR Primer, Index 29 (100% over 44bp)
GGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATC1375370.1042760607359967RNA PCR Primer, Index 29 (100% over 42bp)
TAGTTGGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA1368460.10375216710760161RNA PCR Primer, Index 29 (100% over 39bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1353520.10261946510930606No Hit
GCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT1352430.10253682486980524RNA PCR Primer, Index 29 (100% over 41bp)
GCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGAATTCTCGGGTGCCA1344730.10195303602195543No Hit
CACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACT1324990.10045641370292231RNA PCR Primer, Index 29 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)