FastQCFastQC Report
Sat 23 Sept 2023
SRR15404515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15404515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150154465
Total Bases22.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG90321516.015239706658074RNA PCR Primer, Index 30 (100% over 39bp)
GGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA70389554.687809316892441RNA PCR Primer, Index 7 (100% over 35bp)
AGCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA40763652.7147810756077084RNA PCR Primer, Index 30 (100% over 40bp)
TTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC28303371.8849502743724604RNA PCR Primer, Index 30 (100% over 36bp)
GACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG27698011.844634456924075RNA PCR Primer, Index 30 (100% over 39bp)
AGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG25618561.7061470666223613RNA PCR Primer, Index 30 (100% over 38bp)
GGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA19614151.3062648519975746RNA PCR Primer, Index 30 (100% over 40bp)
AGCCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT17897831.191961224729481RNA PCR Primer, Index 30 (100% over 41bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC17596851.17191653275179No Hit
CTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC15426211.027356062971554RNA PCR Primer, Index 30 (100% over 37bp)
TTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG13487860.8982656626294796RNA PCR Primer, Index 30 (100% over 39bp)
AAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA12912450.8599444578621088RNA PCR Primer, Index 30 (100% over 40bp)
GACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG12601610.839243108754708RNA PCR Primer, Index 30 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA12047280.8023257916439581No Hit
TAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC11497070.7656828586482594RNA PCR Primer, Index 30 (100% over 37bp)
TCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC11425380.7609084418501973RNA PCR Primer, Index 30 (100% over 36bp)
TTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA9297920.619223677431104RNA PCR Primer, Index 7 (100% over 35bp)
TGGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT8728580.5813067230468305RNA PCR Primer, Index 30 (100% over 41bp)
GACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC8621130.5741507586870627RNA PCR Primer, Index 30 (100% over 42bp)
CGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT8078490.5380119732037272RNA PCR Primer, Index 30 (100% over 41bp)
AGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC8047480.5359467665513643RNA PCR Primer, Index 30 (100% over 44bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA7906810.5265784137687813No Hit
TCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA7773700.5177135425177001RNA PCR Primer, Index 30 (100% over 40bp)
ACTCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT7236010.48190441756094293RNA PCR Primer, Index 30 (100% over 41bp)
ACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA7080940.47157705233740466RNA PCR Primer, Index 30 (100% over 40bp)
GCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC6714370.44716419188733414RNA PCR Primer, Index 30 (100% over 36bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC6490540.4322575422582338RNA PCR Primer, Index 30 (100% over 44bp)
AGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT6192110.4123826754002953RNA PCR Primer, Index 30 (100% over 41bp)
CTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT6155620.4099525112356799RNA PCR Primer, Index 30 (100% over 41bp)
TTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC5752530.3831074886784086RNA PCR Primer, Index 30 (100% over 37bp)
TCTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC5750740.3829882781041509RNA PCR Primer, Index 30 (100% over 37bp)
CCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT5663370.3771696033148265RNA PCR Primer, Index 30 (100% over 43bp)
TAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA5635340.3753028589592724RNA PCR Primer, Index 30 (100% over 40bp)
TGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC5596090.37268888407680717RNA PCR Primer, Index 30 (100% over 37bp)
TGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC5401590.3597355563152917RNA PCR Primer, Index 30 (100% over 37bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC5363340.357188179519004No Hit
GAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG5362290.35711825152851767RNA PCR Primer, Index 30 (100% over 38bp)
ATTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC4948120.3295353221764002RNA PCR Primer, Index 30 (100% over 42bp)
CGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT4875520.32470030111991677RNA PCR Primer, Index 30 (100% over 41bp)
TAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT4663660.31059083058236064RNA PCR Primer, Index 30 (100% over 43bp)
AGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG4255960.2834387908478113RNA PCR Primer, Index 30 (100% over 39bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA4192640.2792218000310547No Hit
TCTCGTCTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC4143250.2759325205547501RNA PCR Primer, Index 30 (100% over 37bp)
CAGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA4114490.2740171595962864RNA PCR Primer, Index 30 (100% over 40bp)
CGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC4023050.2679274305962197RNA PCR Primer, Index 30 (100% over 44bp)
CTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG3919450.26102786886823515RNA PCR Primer, Index 30 (100% over 38bp)
AGCCGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC3914710.260712193939754RNA PCR Primer, Index 30 (100% over 42bp)
TGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC3578900.23834789062050205RNA PCR Primer, Index 30 (100% over 37bp)
CGCTGGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG3536040.2354934966469362RNA PCR Primer, Index 30 (100% over 39bp)
GACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA3492330.23258249430011957RNA PCR Primer, Index 30 (100% over 40bp)
CCGGATCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT3440000.22909741645045317RNA PCR Primer, Index 30 (100% over 41bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC3350310.22312423410119706No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTAT3259080.21704849069922763RNA PCR Primer, Index 30 (100% over 48bp)
CGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG3203370.21333831131828146RNA PCR Primer, Index 30 (100% over 45bp)
TCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC3173090.2113217212688281RNA PCR Primer, Index 30 (100% over 42bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG3168640.21102535978533837No Hit
CGGGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT3137050.2089215262429925RNA PCR Primer, Index 30 (100% over 41bp)
CCTAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT3084840.20544444016366747RNA PCR Primer, Index 30 (100% over 41bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3022940.20132201862928284No Hit
CGCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC2958560.19703443384117814RNA PCR Primer, Index 30 (100% over 42bp)
CGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2902660.1933116008238583RNA PCR Primer, Index 1 (100% over 33bp)
CGGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT2898380.19302656101501878RNA PCR Primer, Index 30 (100% over 43bp)
CGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC2880150.19181247790400371RNA PCR Primer, Index 30 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA2818800.18772668531701672No Hit
CCGGTAGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG2772210.18462388048200898RNA PCR Primer, Index 30 (100% over 38bp)
TTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG2761560.18391461086421906RNA PCR Primer, Index 30 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTATGC2737520.18231359287251298RNA PCR Primer, Index 30 (100% over 50bp)
AAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT2721610.18125401732142962RNA PCR Primer, Index 30 (100% over 43bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC2719140.18108952004857132RNA PCR Primer, Index 30 (100% over 42bp)
CGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC2709820.18046882588539742RNA PCR Primer, Index 30 (100% over 42bp)
AGGGGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC2679290.17843558631439965RNA PCR Primer, Index 30 (100% over 42bp)
GCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGAATTCTCGGGTGCCA2671430.17791212535704484No Hit
CTGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC2649340.17644097363338482RNA PCR Primer, Index 30 (100% over 36bp)
AGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT2623750.17473672860810366RNA PCR Primer, Index 30 (100% over 41bp)
ACAGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT2613710.174068083822882RNA PCR Primer, Index 30 (100% over 41bp)
CGCGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA2613190.17403345281806973RNA PCR Primer, Index 30 (100% over 40bp)
ACCGATTGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC2570560.17119437640432472RNA PCR Primer, Index 30 (100% over 37bp)
ACTACCGATTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACC2553890.1700841863077465RNA PCR Primer, Index 30 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG2538560.16906323764664608No Hit
TTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2513640.16740361333910384RNA PCR Primer, Index 1 (100% over 32bp)
ACCGATTGGATGGGACGCTCATCAGACCTGGAATTCTCGGGTGCCAAGGA2458130.1637067535753932RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA2408500.16040149055840597No Hit
ACTGGCGCTGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA2400340.1598580501751979RNA PCR Primer, Index 7 (100% over 35bp)
GGCATGTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG2379400.15846348625064197RNA PCR Primer, Index 30 (100% over 38bp)
NGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG2314070.15411263328066868RNA PCR Primer, Index 30 (100% over 39bp)
GGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG2288060.152380417059193RNA PCR Primer, Index 30 (100% over 45bp)
CTTCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA2228450.14841050514215479RNA PCR Primer, Index 30 (100% over 40bp)
TTGGATTGTTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG2164910.14417886274643915RNA PCR Primer, Index 30 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA2126330.14160950858171284No Hit
TGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGG2065600.13756500680815587RNA PCR Primer, Index 30 (100% over 39bp)
CTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA2034560.1354978022132076RNA PCR Primer, Index 30 (100% over 40bp)
AGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1987130.13233905498581078RNA PCR Primer, Index 1 (100% over 30bp)
AGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT1933890.12879337287772297RNA PCR Primer, Index 30 (100% over 41bp)
TGCGGGTCATAAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1930700.12858092498281684RNA PCR Primer, Index 7 (100% over 35bp)
CGGGGAGAGTTCTCTTTTCTTTGTGATGGAATTCTCGGGTGCCAAGGAAC1926230.12828323153760363RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1908880.12712775474242474No Hit
CTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCGTAT1886560.12564128545894387RNA PCR Primer, Index 30 (100% over 48bp)
AGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTCG1885090.12554338627226302RNA PCR Primer, Index 30 (100% over 45bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT1873540.12477417837691339RNA PCR Primer, Index 30 (100% over 43bp)
CCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1856320.12362735933293759RNA PCR Primer, Index 1 (100% over 33bp)
TAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG1848750.12312321182057423RNA PCR Primer, Index 30 (100% over 38bp)
AGTGGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC1831840.12199703818331342RNA PCR Primer, Index 30 (100% over 42bp)
TCGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA1826690.12165405803949952RNA PCR Primer, Index 30 (100% over 40bp)
NGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1796340.11963280612401368RNA PCR Primer, Index 7 (100% over 35bp)
CAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT1794120.11948495837269973RNA PCR Primer, Index 30 (100% over 41bp)
TGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC1729490.11518072406305067RNA PCR Primer, Index 30 (100% over 44bp)
CGACGCTCATCAGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1715850.11427232616759017RNA PCR Primer, Index 2 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1698550.11312017927672013No Hit
AGCCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCT1684720.11219912774488591RNA PCR Primer, Index 30 (100% over 43bp)
TCCCGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT1654710.11020051917870041RNA PCR Primer, Index 30 (100% over 41bp)
TGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG1639990.10922019535016825RNA PCR Primer, Index 30 (100% over 38bp)
TCGGCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGAT1611600.10732947568359023RNA PCR Primer, Index 30 (100% over 41bp)
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATCTC1599970.10655493994134639RNA PCR Primer, Index 30 (100% over 44bp)
TTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGA1588070.10576242271583466RNA PCR Primer, Index 30 (100% over 40bp)
CGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCGGATC1585360.10558194190229374RNA PCR Primer, Index 30 (100% over 42bp)
TTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGTGGAATTCTCGGGTG1574990.10489131974863351No Hit
CATTCGTGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACCG1564000.10415940678154326RNA PCR Primer, Index 30 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)