FastQCFastQC Report
Tue 26 Sept 2023
SRR15404516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15404516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176664886
Total Bases26.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT89896105.088509778904225RNA PCR Primer, Index 31 (100% over 39bp)
GGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA82652324.678480363098301RNA PCR Primer, Index 7 (100% over 35bp)
TTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC73722654.17302224959407RNA PCR Primer, Index 30 (100% over 36bp)
AGCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA61250313.4670336243275868RNA PCR Primer, Index 31 (100% over 40bp)
AGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA45990542.6032643521474887RNA PCR Primer, Index 31 (100% over 38bp)
GACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT43617722.468952432346969RNA PCR Primer, Index 31 (100% over 39bp)
TAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG23033231.3037808769763108RNA PCR Primer, Index 31 (100% over 37bp)
GACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA21858551.237288886032508RNA PCR Primer, Index 31 (100% over 38bp)
CTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG21221191.2012115412680253RNA PCR Primer, Index 31 (100% over 37bp)
TGGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT18871331.0681992572083623RNA PCR Primer, Index 31 (100% over 41bp)
TTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT18662181.0563604586369246RNA PCR Primer, Index 31 (100% over 39bp)
AGCCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT17328540.9808706411527642RNA PCR Primer, Index 31 (100% over 41bp)
GGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA16603710.9398421144086323RNA PCR Primer, Index 31 (100% over 40bp)
CGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT14672130.8305062954049625RNA PCR Primer, Index 31 (100% over 41bp)
AAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA13730500.777205946856921RNA PCR Primer, Index 31 (100% over 40bp)
TCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA12527280.7090984679321051RNA PCR Primer, Index 31 (100% over 40bp)
TCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC11218430.6350118721385302RNA PCR Primer, Index 30 (100% over 36bp)
CTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT10374910.5872649757915107RNA PCR Primer, Index 31 (100% over 41bp)
TGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG10266840.5811477443231136RNA PCR Primer, Index 31 (100% over 37bp)
TTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG10031640.5678344025875068RNA PCR Primer, Index 31 (100% over 37bp)
TTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA9156780.5183135260959555RNA PCR Primer, Index 7 (100% over 35bp)
AGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC8655740.48995248552108994RNA PCR Primer, Index 31 (100% over 44bp)
GACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC8312360.4705156858392335RNA PCR Primer, Index 31 (100% over 42bp)
TAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT7372250.4173013758942454RNA PCR Primer, Index 31 (100% over 43bp)
AGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT6979560.3950734160041288RNA PCR Primer, Index 31 (100% over 41bp)
GCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC6961110.394029065855226RNA PCR Primer, Index 30 (100% over 36bp)
TCTCGTCTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG6773010.38338178872738754RNA PCR Primer, Index 31 (100% over 37bp)
TAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA6638560.37577133466126367RNA PCR Primer, Index 31 (100% over 40bp)
TGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG6182320.3499461687027042RNA PCR Primer, Index 31 (100% over 37bp)
GAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA6115760.346178583558478RNA PCR Primer, Index 31 (100% over 38bp)
AGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT5734660.32460666801664256RNA PCR Primer, Index 31 (100% over 39bp)
CGGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT5616810.3179358460628107RNA PCR Primer, Index 31 (100% over 43bp)
ACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA5542280.31371712429599624RNA PCR Primer, Index 31 (100% over 40bp)
CGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT5125700.2901368866249969RNA PCR Primer, Index 31 (100% over 41bp)
TCTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG5009740.2835730468815405RNA PCR Primer, Index 31 (100% over 37bp)
CCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT4949460.2801609370183501RNA PCR Primer, Index 31 (100% over 43bp)
CAGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA4915250.27822450240621105RNA PCR Primer, Index 31 (100% over 40bp)
ATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA4905170.2776539306175422RNA PCR Primer, Index 7 (100% over 35bp)
TTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4738370.2682123260193313RNA PCR Primer, Index 1 (100% over 32bp)
TTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA4575200.25897619519025417RNA PCR Primer, Index 31 (100% over 38bp)
CGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4572260.25880977841855907RNA PCR Primer, Index 1 (100% over 33bp)
ACAGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT4498360.2546267173885364RNA PCR Primer, Index 31 (100% over 41bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC4254690.24083393685828433RNA PCR Primer, Index 31 (100% over 44bp)
CGGGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT4210760.23834730802135745RNA PCR Primer, Index 31 (100% over 41bp)
CTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA4074770.2306496832652981RNA PCR Primer, Index 31 (100% over 38bp)
CGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC4042590.22882815547171043RNA PCR Primer, Index 31 (100% over 44bp)
CCGGATCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT4034720.22838267928353342RNA PCR Primer, Index 31 (100% over 41bp)
CGGGTTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT3962470.22429301542129884RNA PCR Primer, Index 31 (100% over 41bp)
TCGGCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT3858570.21841182406785692RNA PCR Primer, Index 31 (100% over 41bp)
CCTAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT3815140.21595349740298705RNA PCR Primer, Index 31 (100% over 41bp)
CGGGGAGAGTTCTCTTTTCTTTGTGATGGAATTCTCGGGTGCCAAGGAAC3671070.2077985095464868RNA PCR Primer, Index 1 (100% over 24bp)
TGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG3652440.20674397061564345RNA PCR Primer, Index 31 (100% over 37bp)
CGCTGGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT3570060.2020809047475343RNA PCR Primer, Index 31 (100% over 39bp)
CGCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC3559240.20146844574422104RNA PCR Primer, Index 31 (100% over 42bp)
GACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA3548100.2008378733507914RNA PCR Primer, Index 31 (100% over 40bp)
AGCCGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC3502990.19828445138781003RNA PCR Primer, Index 31 (100% over 42bp)
TTTTGATCCTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC3482430.19712066607282674RNA PCR Primer, Index 30 (100% over 36bp)
AAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT3404720.1927219424917298RNA PCR Primer, Index 31 (100% over 43bp)
CAGCCTCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA3335340.1887947330971023RNA PCR Primer, Index 7 (100% over 35bp)
CTGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC3312720.1875143428332442RNA PCR Primer, Index 30 (100% over 36bp)
GGGTCGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG3291370.18630584008641082RNA PCR Primer, Index 1 (100% over 29bp)
CTTCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA3245010.183681662693287RNA PCR Primer, Index 31 (100% over 40bp)
CATTCGTGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA3238580.18331769676063414RNA PCR Primer, Index 31 (100% over 38bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC3161120.1789331242655657No Hit
CTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA3118370.17651328855469334RNA PCR Primer, Index 31 (100% over 40bp)
CGTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC3097910.1753551636741214RNA PCR Primer, Index 31 (100% over 42bp)
ACAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA3062930.1733751437170146RNA PCR Primer, Index 31 (100% over 40bp)
GACTGAACGCCTCTAAGTCATGGAATTCTCGGGTGCCAAGGAACTCCAGT3010420.17040284960758983RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2884110.16325315490255374RNA PCR Primer, Index 31 (100% over 41bp)
CAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2831370.16026784179398276RNA PCR Primer, Index 31 (100% over 41bp)
CCCTGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2826680.16000236742008822RNA PCR Primer, Index 31 (100% over 41bp)
TCCCGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2815880.15939104050365732RNA PCR Primer, Index 31 (100% over 41bp)
TCGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA2804860.1587672606315213RNA PCR Primer, Index 31 (100% over 40bp)
ATTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2802560.15863707064005916RNA PCR Primer, Index 31 (100% over 42bp)
AGGGGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2795960.15826348196890694RNA PCR Primer, Index 31 (100% over 42bp)
CGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG2791080.15798725276963074RNA PCR Primer, Index 31 (100% over 45bp)
CTCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT2744180.15533250903068538RNA PCR Primer, Index 31 (100% over 39bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTAT2742800.1552543950358081RNA PCR Primer, Index 31 (100% over 48bp)
TAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA2728560.15444834917562508RNA PCR Primer, Index 31 (100% over 38bp)
TTGGATTGTTCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA2570560.14550486280561717RNA PCR Primer, Index 31 (100% over 38bp)
ACTCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2549400.14430711488416548RNA PCR Primer, Index 31 (100% over 41bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC2547090.1441763588492622RNA PCR Primer, Index 31 (100% over 50bp)
CGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC2535500.14352031450098124RNA PCR Primer, Index 31 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2499700.141493878981701No Hit
TCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2459260.13920479930573187RNA PCR Primer, Index 31 (100% over 42bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2451660.13877460629046567RNA PCR Primer, Index 31 (100% over 42bp)
ACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT2436710.13792837134596175RNA PCR Primer, Index 31 (100% over 39bp)
CCGGTAGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA2229970.12622598924383874RNA PCR Primer, Index 31 (100% over 38bp)
CCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2218510.1255773034602926RNA PCR Primer, Index 1 (100% over 33bp)
CACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA2217980.1255473031579122RNA PCR Primer, Index 31 (100% over 38bp)
AGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC2212740.12525069639475497RNA PCR Primer, Index 31 (100% over 44bp)
GCTGCGTGCTTGATGCTTGTCCCTTTTGATCGTGGAATTCTCGGGTGCCA2209770.1250825814927365No Hit
CGACTCTTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT2202750.1246852189970564RNA PCR Primer, Index 31 (100% over 39bp)
CCGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA2196570.12433540415043202RNA PCR Primer, Index 31 (100% over 40bp)
CGACCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2196100.12430880010869845RNA PCR Primer, Index 31 (100% over 41bp)
GGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2114440.11968648936835133RNA PCR Primer, Index 31 (100% over 42bp)
AGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT2082290.11786665970508706RNA PCR Primer, Index 31 (100% over 41bp)
CGCGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA2074820.11744382525455568RNA PCR Primer, Index 31 (100% over 40bp)
TGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC2061940.11671476130236769RNA PCR Primer, Index 31 (100% over 44bp)
CTTGGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2060400.11662759061243216RNA PCR Primer, Index 31 (100% over 42bp)
GGCATGTTGGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA2041590.11556286289964833RNA PCR Primer, Index 31 (100% over 38bp)
CGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2029710.11489040329157431RNA PCR Primer, Index 31 (100% over 42bp)
GGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG2017950.11422473620479397RNA PCR Primer, Index 31 (100% over 45bp)
TGGTGGTGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA2002220.11333434987188117RNA PCR Primer, Index 31 (100% over 40bp)
CTAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC2000040.11321095240171271RNA PCR Primer, Index 31 (100% over 42bp)
AGTGGAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC1993490.11284019394776618RNA PCR Primer, Index 31 (100% over 42bp)
TGCGGGTCATAAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1974820.11178339084315828RNA PCR Primer, Index 7 (100% over 35bp)
AGCCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT1951300.1104520566695976RNA PCR Primer, Index 31 (100% over 43bp)
CTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA1929530.10921978009823638RNA PCR Primer, Index 31 (100% over 40bp)
TGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT1910400.10813693899533607RNA PCR Primer, Index 31 (100% over 39bp)
TGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA1889520.10695504029023628RNA PCR Primer, Index 31 (100% over 38bp)
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC1884210.10665447122299108RNA PCR Primer, Index 31 (100% over 44bp)
GGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1863660.10549125195144893RNA PCR Primer, Index 1 (100% over 32bp)
CAGGCGGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT1861430.10536502426407475RNA PCR Primer, Index 31 (100% over 39bp)
GCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT1814020.10268141230963125RNA PCR Primer, Index 31 (100% over 41bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC1809830.10244424010779368RNA PCR Primer, Index 31 (100% over 44bp)
ACTGGCGCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG1787780.10119611432008056RNA PCR Primer, Index 31 (100% over 37bp)
TAGGGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT1782790.10091365864295183RNA PCR Primer, Index 31 (100% over 41bp)
CCTAGGGCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT1769850.10018119843011701RNA PCR Primer, Index 31 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)