FastQCFastQC Report
Sat 23 Sept 2023
SRR15404517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15404517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137362199
Total Bases20.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA71965825.239128415525729RNA PCR Primer, Index 32 (100% over 40bp)
GGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA53689223.908587689397722RNA PCR Primer, Index 7 (100% over 35bp)
TTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC52893413.8506525365104265RNA PCR Primer, Index 30 (100% over 36bp)
GGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA51011903.713678171386875RNA PCR Primer, Index 32 (100% over 39bp)
AGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC43414913.1606155344091427RNA PCR Primer, Index 32 (100% over 38bp)
GACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA31774312.3131771499959752RNA PCR Primer, Index 32 (100% over 39bp)
TAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT20029351.4581413333372741RNA PCR Primer, Index 32 (100% over 37bp)
TGGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT16072391.1700737260328804RNA PCR Primer, Index 32 (100% over 41bp)
AGCCGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT15499961.1284006890425509RNA PCR Primer, Index 32 (100% over 41bp)
CTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT12328380.8975089282022924RNA PCR Primer, Index 32 (100% over 37bp)
CGGGGAGAGTTCTCTTTTCTTTGTGATGGAATTCTCGGGTGCCAAGGAAC11630050.8466703419621289RNA PCR Primer, Index 1 (100% over 24bp)
CGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT11456810.8340584297139856RNA PCR Primer, Index 32 (100% over 41bp)
TTAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA11436300.8325652969489808RNA PCR Primer, Index 32 (100% over 39bp)
GACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC10313890.7508535881840389RNA PCR Primer, Index 32 (100% over 38bp)
AAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA9476470.6898892176296624RNA PCR Primer, Index 32 (100% over 40bp)
GGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA8303270.6044799850648868RNA PCR Primer, Index 32 (100% over 40bp)
CCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT8255170.6009782938900098RNA PCR Primer, Index 32 (100% over 43bp)
GGGTCGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAG8249000.6005291164565588RNA PCR Primer, Index 1 (100% over 29bp)
TTAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT7976280.5806750370966324RNA PCR Primer, Index 32 (100% over 37bp)
GACTGAACGCCTCTAAGTCATGGAATTCTCGGGTGCCAAGGAACTCCAGT7683190.5593380170042269RNA PCR Primer, Index 1 (100% over 30bp)
TGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT6585620.47943466601026097RNA PCR Primer, Index 32 (100% over 37bp)
CGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6008760.43743912399072765RNA PCR Primer, Index 1 (100% over 33bp)
AGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC5872630.42752882836419936RNA PCR Primer, Index 32 (100% over 44bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC5313900.3868531545567351RNA PCR Primer, Index 32 (100% over 44bp)
GACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC5274250.38396662534501214RNA PCR Primer, Index 32 (100% over 42bp)
AGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA5072560.36928354648719625RNA PCR Primer, Index 32 (100% over 39bp)
TTTGGTGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4970670.36186593081550766RNA PCR Primer, Index 1 (100% over 32bp)
TCTCGTCTGATCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT4829530.3515909060250266RNA PCR Primer, Index 32 (100% over 37bp)
ACCGATTGGATGGGACGCTCATCAGACCTGGAATTCTCGGGTGCCAAGGA4731630.34446376328031847RNA PCR Primer, Index 1 (100% over 22bp)
CGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC4591280.3342462506733748RNA PCR Primer, Index 32 (100% over 44bp)
TAGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA4468370.32529837411819534RNA PCR Primer, Index 32 (100% over 40bp)
AGACCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT4286780.31207857993013055RNA PCR Primer, Index 32 (100% over 41bp)
CAGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA4231550.30805782309876967RNA PCR Primer, Index 32 (100% over 40bp)
CCGGATCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT3974500.2893445233793906RNA PCR Primer, Index 32 (100% over 41bp)
CGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT3911230.28473845268012926RNA PCR Primer, Index 32 (100% over 41bp)
GAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC3833340.27906804258426293RNA PCR Primer, Index 32 (100% over 38bp)
TTTGGTGTATGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA3754580.2733342962862731RNA PCR Primer, Index 7 (100% over 35bp)
TCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC3723930.27110296916548343RNA PCR Primer, Index 30 (100% over 36bp)
TTAGACCGTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC3637470.2648086610785839RNA PCR Primer, Index 32 (100% over 38bp)
GCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC3564370.25948696409555877RNA PCR Primer, Index 30 (100% over 36bp)
CGGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT3550220.25845684080814696RNA PCR Primer, Index 32 (100% over 43bp)
AGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3543090.2579377751516631RNA PCR Primer, Index 1 (100% over 30bp)
TGTATGTGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT3510460.2555623035708682RNA PCR Primer, Index 32 (100% over 37bp)
GACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA3463190.2521210365888216RNA PCR Primer, Index 32 (100% over 40bp)
ACCGATTGGATGGGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACT3279140.2387221538292351RNA PCR Primer, Index 1 (100% over 25bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC3185960.23193862818110533RNA PCR Primer, Index 32 (100% over 42bp)
AGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2993670.21793987150715313RNA PCR Primer, Index 32 (100% over 41bp)
CGCTGGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA2972510.21639941859113657RNA PCR Primer, Index 32 (100% over 39bp)
CGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCG2938460.2139205706804388RNA PCR Primer, Index 32 (100% over 45bp)
CGTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC2914710.2121915651626981RNA PCR Primer, Index 32 (100% over 42bp)
CTTCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA2852900.20769178280263262RNA PCR Primer, Index 32 (100% over 40bp)
TAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT2749560.2001686067940715RNA PCR Primer, Index 32 (100% over 43bp)
CGACCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2732950.19895939493513787RNA PCR Primer, Index 32 (100% over 41bp)
CCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2730700.19879559441240455RNA PCR Primer, Index 1 (100% over 33bp)
CGCGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA2678770.19501507834771922RNA PCR Primer, Index 32 (100% over 40bp)
ACTCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2646860.19269202293419896RNA PCR Primer, Index 32 (100% over 41bp)
TAGACCGTCGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC2593700.18882196258375278RNA PCR Primer, Index 32 (100% over 38bp)
TTTTGATCCTTCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC2556110.1860854018506212RNA PCR Primer, Index 30 (100% over 36bp)
CGTGTGCCTACCCTACGCCGGCATGGAATTCTCGGGTGCCAAGGAACTCC2507740.1825640546130162RNA PCR Primer, Index 1 (100% over 27bp)
CGGGTGGTAAGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACT2470690.17986680600534066RNA PCR Primer, Index 1 (100% over 25bp)
CGGGTGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2442610.17782257548162866RNA PCR Primer, Index 32 (100% over 41bp)
TCGGATAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA2343270.17059060040237126RNA PCR Primer, Index 32 (100% over 40bp)
TCGGCTACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2308840.16808408840338965RNA PCR Primer, Index 32 (100% over 41bp)
CGCTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC2226320.16207661323185427RNA PCR Primer, Index 32 (100% over 42bp)
AGCAGAACTGGCGCTGCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG2225750.1620351170994285RNA PCR Primer, Index 1 (100% over 29bp)
CGGTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC2207000.16067011274331738RNA PCR Primer, Index 32 (100% over 42bp)
CGGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC2176680.15846280969919532RNA PCR Primer, Index 32 (100% over 44bp)
CATTCGTGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC2061140.15005147085625792RNA PCR Primer, Index 32 (100% over 38bp)
ACAGCCTCTGGCATGTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT2029690.14776190354960755RNA PCR Primer, Index 1 (100% over 30bp)
AAGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT2021260.1471481975911RNA PCR Primer, Index 32 (100% over 43bp)
ACAGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT2000880.14566452885629766RNA PCR Primer, Index 32 (100% over 41bp)
CTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1999860.1455902726193252RNA PCR Primer, Index 32 (100% over 41bp)
CGGGTTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1993270.14511051908829736RNA PCR Primer, Index 32 (100% over 41bp)
GGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1985230.1445252052203969RNA PCR Primer, Index 1 (100% over 32bp)
ACTACCGATTGGATGGGACTGAACGCCTTGGAATTCTCGGGTGCCAAGGA1967510.14323518510358152RNA PCR Primer, Index 1 (100% over 22bp)
CTGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC1957010.1424707826641593RNA PCR Primer, Index 30 (100% over 36bp)
TGAAAATGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT1952530.14214463762333915RNA PCR Primer, Index 32 (100% over 37bp)
TGGTGGTGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1948080.1418206765894888RNA PCR Primer, Index 32 (100% over 40bp)
CCTAGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1920380.13980411015406063RNA PCR Primer, Index 32 (100% over 41bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA1918050.13963448561274125No Hit
TCGGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1912780.13925082838838362RNA PCR Primer, Index 32 (100% over 42bp)
TCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1878800.13677707649394866RNA PCR Primer, Index 32 (100% over 40bp)
CGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1859310.1353581999659164RNA PCR Primer, Index 32 (100% over 42bp)
ATTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1774830.12920803633902223RNA PCR Primer, Index 32 (100% over 42bp)
CAATGTAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1770880.1289204754213348RNA PCR Primer, Index 32 (100% over 41bp)
AGGGTCGGGCCTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAACTCCA1769410.1288134590798157RNA PCR Primer, Index 1 (100% over 28bp)
GGCCTGGTTAGTACTTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1747320.127205301947736RNA PCR Primer, Index 1 (100% over 31bp)
ACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA1739040.12660251602407735RNA PCR Primer, Index 32 (100% over 39bp)
GCCGGATCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1699930.1237552989378104RNA PCR Primer, Index 32 (100% over 41bp)
CGCTAGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTC1693460.12328428143466166RNA PCR Primer, Index 1 (100% over 26bp)
TGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC1690250.12305059268889543RNA PCR Primer, Index 32 (100% over 44bp)
ACCGATTGGATGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT1682700.1225009509348347RNA PCR Primer, Index 32 (100% over 37bp)
CTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC1677470.1221202057197701RNA PCR Primer, Index 32 (100% over 38bp)
GCTAGAGGTGAAATTCTTGGACCTGGAATTCTCGGGTGCCAAGGAACTCC1661730.12097433006296006RNA PCR Primer, Index 1 (100% over 27bp)
TATCATTGTGAAGCAGAATTCACCATGGAATTCTCGGGTGCCAAGGAACT1646730.11988232657807116RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGAATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1644190.11969741398796332RNA PCR Primer, Index 32 (100% over 42bp)
AGCCGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1639030.11932176478916155RNA PCR Primer, Index 32 (100% over 42bp)
CCGGCCGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1619640.11791016828436184RNA PCR Primer, Index 32 (100% over 40bp)
CCCTGAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1616170.11765755147819087RNA PCR Primer, Index 32 (100% over 41bp)
TCCCGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1614190.11751340701818555RNA PCR Primer, Index 32 (100% over 41bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC1606090.11692372513634555RNA PCR Primer, Index 32 (100% over 44bp)
GGGGATCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1598510.11637189937531503RNA PCR Primer, Index 32 (100% over 42bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT1583910.11530901598335652RNA PCR Primer, Index 32 (100% over 43bp)
GGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCG1573140.11452495748120631RNA PCR Primer, Index 32 (100% over 45bp)
TGACTGAACGCCTCTAAGTCAGATGGAATTCTCGGGTGCCAAGGAACTCC1557090.11335651375237521RNA PCR Primer, Index 1 (100% over 27bp)
CACGGACCAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTC1539570.112081053682025RNA PCR Primer, Index 32 (100% over 38bp)
CAGCCTCTGGCATGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1534230.11169230044140455RNA PCR Primer, Index 7 (100% over 35bp)
TGGGATTAGACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1527110.11117396278724397RNA PCR Primer, Index 1 (100% over 31bp)
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTC1490140.10848253819815451RNA PCR Primer, Index 32 (100% over 44bp)
CTGAACGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1489770.1084556021121939RNA PCR Primer, Index 32 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1484440.10806757687389673No Hit
AGGCGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAT1483410.10799259263460101RNA PCR Primer, Index 32 (100% over 41bp)
TCTGGCATGTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACT1433460.10435622102992104RNA PCR Primer, Index 32 (100% over 37bp)
CGTCATCTAATTAGTGACGCGCATGGAATTCTCGGGTGCCAAGGAACTCC1430570.10414582835849913RNA PCR Primer, Index 1 (100% over 27bp)
CTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1419450.10333628977503484RNA PCR Primer, Index 32 (100% over 40bp)
AGGGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT1410400.10267744767248521RNA PCR Primer, Index 32 (100% over 43bp)
CGGACGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATC1402660.10211397387428255RNA PCR Primer, Index 32 (100% over 42bp)
TGACTGAACGCCTCTAAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1401510.10203025360710774RNA PCR Primer, Index 1 (100% over 31bp)
ACGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCT1383130.10069218533695723RNA PCR Primer, Index 32 (100% over 43bp)
CGACGCTCATCAGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1380570.10050581674220285RNA PCR Primer, Index 2 (100% over 34bp)
ATGGATTTTTGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1376280.10019350374552465RNA PCR Primer, Index 7 (100% over 35bp)
ACCGTCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA1374150.10003843925067042RNA PCR Primer, Index 32 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)