FastQCFastQC Report
Sat 16 Sept 2023
SRR15410424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15410424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15891365
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA523730.3295689199763519Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA354210.22289463491650968Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA321790.2024936183896097Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA261900.16480648452791816Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA233630.14701694914187674Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA220360.13866650221676993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA214370.13489715955803672Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA209830.13204026211719383Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA194810.12258858820497799Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA183140.11524497738237087Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA180320.11347042875171516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA172570.10859356638023229Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA171290.10778809749823254Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA167580.10545349628556137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA167490.10539686175479576Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA166200.1045851001471554Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA162130.10202396081142182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)