FastQCFastQC Report
Sat 16 Sept 2023
SRR15410426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15410426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15022941
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGATACTGTAGGCACCATC296790.19755785501653772No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGATCTGTAGGCACCATC281170.18716042351494291No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGAACTGTAGGCACCATC266800.17759505279292517No Hit
ACAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA239450.15938956293577936Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTACAACTGTAGGCACCATC207580.13817534129968292No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGCAACTGTAGGCACCATC180810.12035592764426087No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGACACTGTAGGCACCATC176430.11744038667262288No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGTAACTGTAGGCACCATC163560.1088734888860976No Hit
TCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA154620.10292259019056256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)