FastQCFastQC Report
Sat 16 Sept 2023
SRR15410427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15410427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18687423
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGATACTGTAGGCACCATC295860.15832038478499685No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGATCTGTAGGCACCATC273680.14645143955910883No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGAACTGTAGGCACCATC245480.13136107637741168No Hit
ACAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA211720.11329545010031614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTACAACTGTAGGCACCATC189590.10145326083751621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)