FastQCFastQC Report
Sat 16 Sept 2023
SRR15410428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15410428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23746716
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGATACTGTAGGCACCATC312890.13176137702577484No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGATCTGTAGGCACCATC312410.1315592438129129No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTGGAACTGTAGGCACCATC265940.11199022214271649No Hit
ACAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA254970.10737063600710095Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)