FastQCFastQC Report
Sat 16 Sept 2023
SRR15446778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16966114
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA9519145.6106778487990825No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC4026932.3735134633658594No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC2688131.5844111385789346No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC2358391.3900590317853576No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1723231.0156892733362515No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1321950.7791707635584671TruSeq Adapter, Index 6 (96% over 27bp)
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1304210.7687146272858947No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1218680.7183023761363385No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1129070.6654853315261232No Hit
ATCTCCGGGGGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC1091800.6435180147911301RNA PCR Primer, Index 6 (96% over 26bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1024090.6036090527271006Illumina PCR Primer Index 6 (96% over 29bp)
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA979420.577280100793853No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT940560.5543756219013971No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA910430.5366166937225578No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT765820.4513820902063961No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA729920.4302222653932421No Hit
TGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATCT723310.4263262642229092No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC721230.4251002910861026RNA PCR Primer, Index 6 (96% over 26bp)
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC718270.4233556370068007No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT709270.4180509455494641No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC701880.41369520445282876No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC634390.37391591262442303No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC633500.3733913375803086No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA629310.3709217090018374No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA624890.3683165160861232No Hit
AAAACGGCGGCCGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT592980.34950843781905505Illumina PCR Primer Index 6 (100% over 23bp)
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA590700.34816458264986316No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC577370.3403077451913856No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT538310.31728538426654446No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT532560.31389627583546825No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA522080.3077192573384807No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC510090.3006522294969844No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA500060.29474044557286366No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA499440.29437501127246934No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT475260.2801230735570915No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCA449220.2647748329405307No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT445270.2624466628009219No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT436020.25699461880310365No Hit
TCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATA430630.2538176980303209No Hit
AAAACGGCGGCCGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG421260.2482949248130715Illumina PCR Primer Index 6 (96% over 25bp)
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT400760.23621201649358242Illumina PCR Primer Index 6 (100% over 23bp)
ATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT380760.2244238132550565TruSeq Adapter, Index 6 (95% over 21bp)
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT378820.2232803575409195No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT377920.22274988839518584No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC358740.21144500148943945No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA348550.2054389119394105No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT347310.20470804333862191No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC337490.19892003554850568No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC326060.19218307739768814No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC318000.18743243149256217No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC317960.18740885508608512No Hit
AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCCTGTAGGCACCATCAA307860.18145581245062953No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT307000.18094891971137292RNA PCR Primer, Index 6 (96% over 28bp)
TTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATAT302250.178149221442223No Hit
TTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATC293970.17326890530147326No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC290710.17134742817359355No Hit
TGATCGAAGCCCCGGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTT282910.16675002891056845TruSeq Adapter, Index 6 (95% over 22bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT271770.1601839997067095No Hit
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT266980.15736072503108256No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC264950.15616422240237215No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT261860.15434294500201992No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTCTGTAGGCACCATCAATATCTC260080.1532937949137911No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA259040.15268080834538775No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA251040.1479655270499774No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCCTGTAGGCACCATCAATA249550.1470873059087072No Hit
AGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT245670.1448003944804332TruSeq Adapter, Index 6 (95% over 22bp)
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA243640.1436038918517228No Hit
GAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATCTCGTAT230850.13606533588068548No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT230180.13567043107219484No Hit
TAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC228550.13470969250825499No Hit
CACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC228290.13455644586615415RNA PCR Primer, Index 6 (96% over 26bp)
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC227130.13387273007831962No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA225810.13309470866457696No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT224810.13250529850265064No Hit
ACCGAGCGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATAT222370.13106713770755046No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA218900.12902188444566623No Hit
CTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC217820.12838532147078582No Hit
AAAAACGGCGGCCGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC217340.12810240459306121RNA PCR Primer, Index 6 (95% over 23bp)
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAA212740.12539111784820023No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT212270.12511409507209487No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA210970.12434786186159069No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA210740.12421229752434765No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG210310.12395885115471936No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT208390.12282718364382086Illumina PCR Primer Index 6 (95% over 24bp)
AGCGAAGCTCTTGATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT206910.12195485660416994Illumina PCR Primer Index 6 (95% over 22bp)
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC201050.11850091305528185No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA197890.11663837694359475No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC196570.11586035552985204No Hit
TGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCA193290.11392709019873379No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA193240.11389761969063747No Hit
AAAATGCCCTGCTTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT191180.1126834347570693Illumina PCR Primer Index 6 (95% over 22bp)
CGAAGCCCCGGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC190770.11244177659067951TruSeq Adapter, Index 6 (96% over 26bp)
GATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG187490.11050851125956128Illumina PCR Primer Index 6 (96% over 25bp)
GAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC187330.11041420563365305No Hit
AAGGCTGAGGCGTGATGACCTGTAGGCACCATCAATATCTCGTATGCCGT187180.11032579410936412No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA186000.10963029011829108No Hit
AGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT184100.10851041081063112RNA PCR Primer, Index 6 (95% over 24bp)
TGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAATATCTC179400.10574018304957754No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA178520.10522150210708239No Hit
CAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC174350.10276366173184973No Hit
ATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATATCTC171860.10129603042865326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)