FastQCFastQC Report
Sat 16 Sept 2023
SRR15446779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18076411
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC3760682.080435104070161No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA3694522.0438349183363886No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC3429191.8970524624606067No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT3136051.7348853154533828No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC2538061.4040729655903486No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC2210701.2229750695533532No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG2127961.1772027090997212No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT1895361.0485267235846762No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA1865121.0317977390533994No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA1683410.9312744659324242No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1574530.8710412703052614Illumina PCR Primer Index 6 (96% over 29bp)
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC1534040.8486419123796201No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC1454020.8043742754023462No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC1375170.7607538908027706No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC1374010.7601121704966766No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA1372950.7595257709066252No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCAA1203030.6655248102070704No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC1190340.6585046113412668No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA1183660.6548091875096224No Hit
ACCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT1172310.6485302862387893Illumina PCR Primer Index 6 (95% over 21bp)
AGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCT1135630.6282386475943704No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1126740.6233206359381849No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT1121180.6202448041262174No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1115470.6170859912401859No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1007930.5575940932079936No Hit
TCCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC957660.5297843692533878No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC728700.40312205780229277No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC714810.39543801034397813No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC704080.3895020975126091No Hit
TAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC690670.3820835894912989No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA678450.3753233979908954No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA657300.3636230665478894No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT655460.3626051653727059No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT628670.3477847455448983No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA609090.33695294934376074No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC600230.332051533902388No Hit
AAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATA571560.31619108461297984No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC565440.3128054567911739No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA555870.3075112642658988No Hit
AACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCA541690.29966678673106073No Hit
TTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTA541500.2995616773705798No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC534680.29578880453647577No Hit
ATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC517950.2865336487425518No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCA502990.2782576696225816No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT498220.2756188714673505No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCA493660.27309624681580874No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA490810.2715196064085951No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT477740.2642891888218297No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT477160.2639683286687827No Hit
ACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA468770.25932692059281015No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA458560.25367867548486256No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC454960.2516871297073296No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC443550.24537503600687108No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT437050.24177918946410326No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC435980.24118725780244762No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA427410.23644627243759836No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT423650.23436621351439732No Hit
ACCTGCGGTTGGATCACTGTAGGCACCATCAATATCTCGTATGCCGTCTT415320.22975799786805026TruSeq Adapter, Index 6 (95% over 22bp)
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT410700.22720218078688298No Hit
TTGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATC404560.22380548882186846No Hit
AAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC402790.2228263121479148TruSeq Adapter, Index 6 (95% over 23bp)
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT382090.21137492392710033No Hit
ATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACC379040.2096876420878016No Hit
AAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC359180.1987009478817449No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC353420.1955144746376922No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT351090.19422550195390004No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA344300.19046922533460872No Hit
TCCTGCGGTTGGATCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT341290.188804071781727Illumina PCR Primer Index 6 (95% over 21bp)
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT325330.17997488550133098No Hit
CAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC312200.17271127548494No Hit
ACCCCGTGAACCTTTACTATAGCTTGACACTGAACACTGTAGGCACCATC308360.17058695998890488No Hit
GAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC307060.16986779068035132Illumina PCR Primer Index 6 (100% over 22bp)
CAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT302260.1672123963103074No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG292140.16161393984679812No Hit
GGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTT284810.1575589313608769TruSeq Adapter, Index 6 (95% over 22bp)
CCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTCT278010.15379712266998133Illumina PCR Primer Index 6 (95% over 21bp)
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT276760.15310561371944906No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA274800.15202132768501447No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA266860.14762886283123347No Hit
TTCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATA266550.14745736861150147No Hit
TTATCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCA261010.14439260094274245No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA256240.14175380278751132No Hit
AACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCCTGTAGGCACCA247610.1369796249930365No Hit
AAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC247390.13685791941774283No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA245720.13593406345983172No Hit
AAAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAA243530.13472253977849916No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA243130.13450125691432885No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC241850.1337931517489838No Hit
TGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCACC239330.13239906970471074No Hit
TGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTAT235030.13002027891487974No Hit
TAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC227450.12582696863885204No Hit
AAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCAC227410.125804840352435No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC227030.1255946216314732No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT226940.12554483298703487No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT226830.12548398019938803No Hit
CCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGT225630.12482013160687705No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCATGCCTGTAGGCAC224570.12423373201682568No Hit
GCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATG222580.12313284976757831No Hit
ACGATGTCGACTTGGAGGTTGTGCCTGTAGGCACCATCAATATCTCGTAT215010.11894507156315487No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC209960.1161513754030045No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCATCAA209090.11567008517343405No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA204670.11322490952435194No Hit
CTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA201460.11144911453938507No Hit
TCTGCCGTGGGCGCTGGAGAACTGAGGGGGGCTGCTCCCTGTAGGCACCA201060.11122783167521473No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCAT200340.11082952251970815No Hit
GAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA197490.10925288211249458No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT195840.10834009029779196No Hit
ACCAGGGCTACACACGTGCTACAATGGCGCATACAAAGCTGTAGGCACCA194820.10777581899415764No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC193750.107183887332502No Hit
TCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCTCGTATGCCG189440.10479956447106674No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGCTGTAGGCACCATCAAT188670.10437359495753887No Hit
GAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC188500.10427954974026646No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC187390.10366548979219381No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCATCAAT184750.10220502288866966No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCTGTAGGCAC184660.10215523424423134No Hit
TCCCCATGCGAGAGTAGGGAACTGCCAGGCACTGTAGGCACCATCAATAT184300.10195607966647804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)