FastQCFastQC Report
Sat 16 Sept 2023
SRR15446780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17572260
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6086573.4637377320845473Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT3364391.9146029025293276No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT2723321.549783579346083No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT2328751.325242171467984No Hit
TGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAG1614830.918965460333503No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1263760.719178978685724No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1157720.6588338665601351No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC994780.56610817276776No Hit
TTGCTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTA967940.5508340987442708No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG817360.4651422184738901No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC815120.46386748204271966No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCCTGTAGGCACCATCAATA738570.4203045026649958No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC664390.3780902399577516No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA647880.36869474956550835No Hit
GATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT632360.35986264714954136No Hit
TATTCATCTCCGGGGGTAGAGCCTGTAGGCACCATCAATATCTCGTATGC594220.33815798309380807No Hit
CATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT575130.32729426949066315No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC566450.3223546658198775No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG526110.29939802848353025No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA516410.2938779644735509No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA464720.26446228316676396No Hit
AAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA409490.23303206303571652No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA409330.23294101043349005No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT400050.22765995950435516No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCTGTAGGCACCATCAATA391890.22301627679080552No Hit
TACTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT389620.22172446799671755No Hit
CTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC326310.18569609145323368No Hit
CAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT325430.18519530214098812No Hit
GAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT324530.18468313125346428No Hit
TGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCA315950.17980043545907015No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA308120.17534454873761257No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC297780.16946027431872734No Hit
TTAACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGC297760.16944889274344907No Hit
AAGGCTGAGGCGTGATGACGAGGCTGTAGGCACCATCAATATCTCGTATG281920.16043468512302914No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATATCTCGT277020.15764619917984368No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA270260.1537992267357756No Hit
AATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT266600.15171639845984525No Hit
ATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAATA264680.15062376723312768No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC262410.1493319584390397No Hit
AACTGGAGGAAGGTGGGGCTGTAGGCACCATCAATATCTCGTATGCCGTC258580.14715238677324374No Hit
AAGGCTGAGGCGTGATGACGAGCTGTAGGCACCATCAATATCTCGTATGC257000.1462532423262574No Hit
GGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTATGC254080.14459153233562444No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCAAGGGGGTCCTGTAGGC250800.14272495398998192No Hit
ACGAAGGGGTCGGAGGTTCGAATCCTCTATCACCGACCACTGTAGGCACC231070.13149702997793114No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA230320.13107022090499457No Hit
ACGGTCCTAAGGTAGCGCTGTAGGCACCATCAATATCTCGTATGCCGTCT229220.13044423426468765Illumina PCR Primer Index 6 (95% over 21bp)
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATATCTCG224620.1278264719506768No Hit
AAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACC218500.12434370991551456No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT210310.11968295483904745No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT200530.11411736452795486No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCACTGTAGGCACC197750.11253532556427005No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT196270.11169308899367526No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT195440.11122075361962548No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT191630.10905256352910782No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA190440.10837535980004849No Hit
AAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAAT185480.10555272913102812No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC185130.10535355156365772No Hit
ACGGGGGCCCGCACAAGCGGTGCTGTAGGCACCATCAATATCTCGTATGC185040.10530233447490532No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC184090.10476170964918571No Hit
ACTTGTGGCTGGGGGTGCTGTAGGCACCATCAATATCTCGTATGCCGTCT183270.10429506506277508Illumina PCR Primer Index 6 (95% over 21bp)
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGCTGTAGGCACC181650.10337315746523214No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA181110.10306585493271782No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC179040.10188786189141294No Hit
ACGGGGCTAAACCATGCACCGAAGCTGCGGCAGCGACGCCTGTAGGCACC176400.10038549395467629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)