FastQCFastQC Report
Sat 16 Sept 2023
SRR15446784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15446784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16965497
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA7672554.5224434038095085No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCAC7406334.365524923908802No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC2033571.1986504138369773No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACC2013061.1865611717711542No Hit
ACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT1474150.8689105895335691TruSeq Adapter, Index 6 (96% over 27bp)
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1274030.7509535382311523No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATC1264270.7452006858390297No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1240050.7309246525462826No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1173500.6916979797290936No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG1166230.6874128120148794No Hit
AGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA1059990.62479159909079No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA1057380.6232531826211752No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC1045980.6165336624090647No Hit
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCAC877720.517355901804704No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGC854680.5037753977970701No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT846110.4987239690060362No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCA837430.49360770273927135No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA763690.45014301673567236Illumina PCR Primer Index 6 (96% over 29bp)
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT760480.4482509413075255No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCTGTAGGCACCATCAATA746260.439869223990314No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT727650.42889990195984234No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT691250.4074445918088931No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT665390.39220189069615824No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT657530.3875689583393873No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCTGTAGGCACCATCA650500.3834252542085858No Hit
AAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCA568980.33537479037602025No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT558830.32939205966085167No Hit
ACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGCTGTAGGCACCATCA552450.32563148606846004No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTGTAGGCACCATCAATAT499530.294438765925926No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT496570.2926940483971675No Hit
GGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA492730.2904306310625619No Hit
TATTCATCTCCGGGGGTAGAGCACTGTTTCGGCCTGTAGGCACCATCAAT480470.2832041996765553No Hit
GGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTAGGCACCATCAATA474150.27947899198001686No Hit
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA458290.27013060684281753No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCCTGTAGGCACCATCAATAT456550.26910499586307435No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC441590.26028709916367315No Hit
GTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAAT429110.25293099282620485No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACCTGTAGGCACCATCA429080.25291330987827826No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA417180.24589907386739096No Hit
GAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCAT392200.23117507256050324No Hit
CGGTGATTGGCGCAGCCTGGTAGCGCACTTCGCTGTAGGCACCATCAATA388890.22922405397260096No Hit
ATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCACTGTAGGCACC362240.21351570189779878No Hit
TAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA346040.20396691001743125No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATCA340300.20058357264747384No Hit
CAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC337820.19912178228554106No Hit
AGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC337370.19885653806664197No Hit
TACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC331380.1953258427972962RNA PCR Primer, Index 6 (96% over 26bp)
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC328460.1936047025324398No Hit
TGCACTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT322660.1901859992666292Illumina PCR Primer Index 6 (100% over 23bp)
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCTGTAGGCACCATCAATA320380.1888420952242071No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCCTGTAGGCACCATCA315040.18569453049327111No Hit
GAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCAC303030.17861545700665296No Hit
ACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCAT298040.17567419333486073No Hit
TTAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCA284410.16764024066020583No Hit
CTGTTTCGGCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT283450.1670743863265544RNA PCR Primer, Index 6 (96% over 28bp)
TGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATCT281360.1658424742876675No Hit
CGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTGTAGGCACCATCAATA280020.16505263594694572No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCCTGCCTGTAGGCACCATCAATAT279700.1648640178357286No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC276220.16281279587624223No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGACCTGTAGGCACCATCAATATC274840.16199938027161834No Hit
CGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCTGTAGGCACCATC274480.16178718489649904No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA272980.16090303750016874No Hit
TTGAAGCAACAAATGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGC264180.15571603944169746No Hit
TTTGCTCGTGGAGCTGAAATCAGTCGAAGATACCAGCTGGCCTGTAGGCA261960.1544075012951286No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCCTGTAGGCACCATCAATA244930.1443694811887916No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC240250.14161094131224095No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT233330.13753207465717038No Hit
GATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG232100.1368070737921795Illumina PCR Primer Index 6 (96% over 25bp)
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT230110.13563410491304792No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA225810.13309954904356766No Hit
TGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCCTGTAGGCACCATCAA225750.13306418314771445No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC225750.13306418314771445No Hit
GAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC223320.13163186436565932No Hit
CGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCTGTAGGCACCATCAA223130.13151987236212415No Hit
TAAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC222150.13094222939652164No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGCTGTAGGCACCATCAATATCTC219990.12966905714580598No Hit
AAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTACTGTAGGCACCAT218670.1288910074370353No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA217670.12830157583948174No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTGTAGGCACCATCAATATCTCG211790.12483571804586685No Hit
CAGGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGC209160.12328551294430101No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC207880.12253104049943246No Hit
ACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGCTGTAGGCACCATCAAT201710.11889424754252705No Hit
TGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT201370.11869384079935884Illumina PCR Primer Index 6 (95% over 24bp)
TGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCCTGTAGGCACCATCA198750.11714953001376854No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT198220.11683713126706516No Hit
TAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGCTGTAGGCACCATC194200.11446761624489987No Hit
TCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATA193790.11422594928990291No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC193400.11399607096685702No Hit
TCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGCTGTAGGCACCATCAAT189580.11174444226420246No Hit
GGGTGATTAGCTCAGCTGGGAGAGCACCTCCCCTGTAGGCACCATCAATA187630.11059505064897303No Hit
AGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCAT183980.10844362531790258No Hit
TAGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCTGTAGGCACCA181840.10718224169913797No Hit
CGCAGGTTCGAATCCTGCACGACCCCTGTAGGCACCATCAATATCTCGTA181030.10670480210511958No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCCTGTAGGCACCATCAATATC177390.10455927109002466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)